Cardiolipin test reuslts

[Guest guest]

Hi,

I have just had my cardiolipin test results back. I asked to have this

test done as I have had a problem with my ATP profile (autumn 2005

showing poor conversion of ADP to ATP and possible blockage of active

site) and the translocator test in March2006 (with nickel glutathione

conjugates and few cristae and an abnormal mitochondrial lining).

Since then I have been doing things to try and remove the nickel and

have not repeated the translocator test.

Summary of results: essential normal findings although around 20% of

the CL-synthase sites are assoicated with nickel rather than the

normal co-factor (manganese).

My doc said that she has only seen 10 results so far and

mine is the first " essentially normal " result. The nickel levels are

probably not that significant and confirms my past nickel problem. The

malondialdehyde levels are manageable indicating good antoxidant

status.

Bit more about test: The structure of mitochondrial membranes is quite

different from cell membranes and this probably reflects the fact that

from an evolutionary point of view, mitochondria are bacteria, not

human cells! What makes them different is the presence of

cardiolipin. The function of mitochondrial membranes is to hold the

bundle of enzymes responsible for oxidative phosphorylation called

cristae in the right configuration so that they can work properly.

Test results (normal ranges are in brackets after result) :

- Percentage of membrane lipids that fluorescence-stain as CL - 23

(19-28%)

- CDP-diacylglycerol pmol/10 6mt(CL-synthesis precursor) - 202 (105-

23-

pmol)

- CL-synthase (units/10 6mt) - 97 (85-130 units)

- CL-synthase manganese sites (no per 10 nM of mt membrane) - 14 (11-

24)

- No of above occupied by metals other than manganese - 3 (metal if

identified - Nickel) (normal range up to 3)

- CL-binding zenobiotics (identified if possible) - not present

(normal range - not present)

- cytochrome-c oxidase molecules (no bound to each CL molecule) - 2

(2)

- gating action of cytochrome-c-CL complex (on pH change) - normal

(normal)

- Malondialdehyde (MDA) concentration pmol/10 6mt - 20 (up to 32)

- Phosphatidylethanolamine and phophatidyl choline (the major

phospholipids in membranes with cardiolipin in the mitochondria

only) - semi quantitative assessment: PTE - normal (normal). PTC -

normal(normal)

Phew thats the lot,

Carol