Guest guest Posted September 18, 2006 Report Share Posted September 18, 2006 In his book, " The Body Electric " O. Becker writes " Bacteria and yeast cells exposed to these NMR [nuclear magnetic resonance] conditions doubled their rate of DNA synthesis and proliferation, but daughter cells were half-size. " p. 297 So if EMR can excellerate the proliferation of bacteria and yeast, then it would also be very possible that it might have the same effect on mold - both inside and outside the body. Since EMR has the effect of knocking out the Natural Killer Cell defense system which attacks yeast, mold, and virally infected cells and cancer cells, then this would certainly contribute to its proliferation in the body. Suppression of natural killer cell activity on Candida stellatoidea by a 50 Hz magnetic field. Canseven AG, Seyhan N, Mirshahidi S, Imir T. Department of Biophysics, Gazi University Medical Faculty, Ankara, Turkey. canseven@... Exposure to electromagnetic fields (EMF) is ubiquitous for almost all individuals living in industrialized countries. Epidemiological and laboratory studies suggest that exposure to Extremely Low Frequency (ELF) EMF increase cancer risk. The immune system functions as one of the body's main protective mechanisms, and Natural Killer (NK) cells are a subset of lymphocytes that can destroy several types of tumor cells. In this study, we investigated, NK cell activity after exposure to a 50 Hertz (Hz), 2 mT magnetic field generated by a Helmholtz Coil. Nineteen male, 10-12 week old guinea pigs were used, and NK cytotoxic activity of splenocytes was measured in vitro by natural anticandidial colorimetric index. The Mann-Whitney U test was applied for statistical analysis. NK cell cytotoxic activity was decreased in exposed compared to controls. Our data suggests that part of the immune system, the NK cell, can be suppressed by a 50 Hz magnetic field. PMID: 16771296 [PubMed - indexed for MEDLINE] Modulation of natural killer cell function after exposure to 60 Hz magnetic fields: confirmation of the effect in mature B6C3F1 mice. House RV, McCormick DL. Life Sciences Department, IIT Research Institute, Chicago, Illinois 60616, USA. In a previous study, we demonstrated that subchronic exposure to pure, linearly polarized 60 Hz magnetic fields (MFs) at flux densities ranging from 0.002 to 1.0 mT induced a modest but statistically significant and reproducible suppression of NK cell activity in young adult B6C3F(1) mice. NK cell activity in mice declines with age and is known to be suboptimal in older animals. The present study was designed to determine if the same MF exposure regimens will suppress NK cell activity in mature (i.e. more than 1 year old) animals. Extending our previous findings, a modest suppressive effect of MFs on NK cell activity in B6C3F(1) mice was found when subchronic exposure was initiated in animals held in quarantine for 1 year prior to exposure. These data demonstrate that MF exposure suppresses NK cell activity in both young and mature adult B6C3F(1) mice. However, because chronic exposure to the same MF parameters used in the NK function studies does not increase the incidence of neoplasia in B6C3F(1) mice, this statistically significant inhibition of NK cell function appears to be of limited biological significance. PMID: 10790298 [PubMed - indexed for MEDLINE] [The effect of electromagnetic radiation with extremely high frequency and low intensity on cytotoxic activity of human natural killer cells] [Article in Russian] Fedorchuk AG, Skivka LM, Stoliarov ZE, Levchuk IuN, Mostovaia AV. EHF electromagnetic radiation under short-time action suppresses the cytotoxical activity of the natural killer cells from granulocyte fraction and peripheral blood of healthy volunteers; the observed effect is non-linear. Under the long-time irradiation of the natural killer cells from the mononuclear fraction of blood, the suppressing effect gets a practically linear character after the 20-30 minutes action. Under the long-time irradiation of peripheral blood the insignificant stimulation of natural killers was observed. It is assumed that the radiation applied can suppress the cytotoxic activity of the natural killers, breaking the normal metabolic pathway of phosphatidylinositphosphate. PMID: 1335293 [PubMed - indexed for MEDLINE] Anti-candidial activity of natural killer (NK) and lymphokine activated killer (LAK) lymphocytes in vitro. Gulay Z, Imir T. Department of Microbiology, Gazi University, Faculty of Medicine, Besevler, Ankara, Turkey. The natural cytotoxic effects of peripheral blood lymphocytes (PBL) on Candida stellatoidea and several other Candida species were examined by a colony forming inhibition (CFI) assay. Peripheral blood mononuclear cells (PBMC), were incubated with C. stellatoidea yeast cells. After the incubation period the colony-forming ability of the yeast was significantly reduced. In similar experiments, six different Candida species (C. albicans, C. krusei, C. stellatoidea, C. tropicalis, C. pseudotropicalis, C. guillermondii) were used as target cells. There was no statistically significant difference in the anticandidial activities of PBL against the Candida species used. It was demonstrated that a fraction of lymphocytes, natural killer cells (NK), had the major natural anti-candidial activity by using anti-Leu M1 (CD 15) and anti-Leu 11b (CD 16) monoclonal antibodies (mAbs) plus complement (C'). It was observed that inhibition of colony-forming ability of C. stellatoidea was significantly (78-96%) reduced when anti-Leu 11b plus C' were used. In addition, the colony formation inhibition capacity of NK cells was increased by recombinant human interleukin-2 (rhIL-2) while anti-interferon-gamma (IFN-gamma) had no effect. Besides the fact that NK cells are among those responsible for natural immunity against Candida species, this colony- forming inhibition assay performed with C. stellatoidea yeast cells as target and monocyte-depleted PBMC as effector cells, is a simple method to assess NK cell activity. PMID: 8877398 [PubMed - indexed for MEDLINE] [Effect of radio-frequency electromagnetic radiation on physiological features of Saccharomyces cerevisiae strain UCM Y-517] [Article in Russian] Voichuk SI, Podgorskii VS, Gromozova EN. Effect of electromagnetic radiation (40.68 MHz) on growth characteristics of Saccharomyces cerevisiae strain UCM Y-517 has been studied. Reliable increase of the specific growth rate (by 7-15%) and change of duration of growth phases as a result of irradiation of yeast population has been shown. The EMR effect has been found to depend on physiological state of the irradiated cells and composition of the irradiation performance medium: reliable effects were found only for the cells preliminarily grown on the dense nutrition medium in the late phase of delayed growth or on achieving the stationary growth phase. The role of radiation term and power as well as of temperature factor in EMR effect on the cells is discussed. It has been noted that the dependence of specific growth rate of yeast on the initial density of cells population acquire the nonlinear character as affected by EMR of radiofrequency range. PMID: 15456218 [PubMed - indexed for MEDLINE] [Effect of radio-frequency electromagnetic radiation on physiological features of Saccharomyces cerevisiae strain UCM Y-517] [Article in Russian] Voichuk SI, Podgorskii VS, Gromozova EN. Effect of electromagnetic radiation (40.68 MHz) on growth characteristics of Saccharomyces cerevisiae strain UCM Y-517 has been studied. Reliable increase of the specific growth rate (by 7-15%) and change of duration of growth phases as a result of irradiation of yeast population has been shown. The EMR effect has been found to depend on physiological state of the irradiated cells and composition of the irradiation performance medium: reliable effects were found only for the cells preliminarily grown on the dense nutrition medium in the late phase of delayed growth or on achieving the stationary growth phase. The role of radiation term and power as well as of temperature factor in EMR effect on the cells is discussed. It has been noted that the dependence of specific growth rate of yeast on the initial density of cells population acquire the nonlinear character as affected by EMR of radiofrequency range. PMID: 15456218 [PubMed - indexed for MEDLINE] tatic magnetic fields enhancement of Saccharomyces cerevisae ethanolic fermentation. da Motta MA, Muniz JB, Schuler A, Da Motta M. Departments of Biophysics and Chemical Engineering, University Federal de Pernambuco, Recife PE 50960.870, Brazil. motta@... Magnetic effects induced in ethanolic fermentation by Saccharomyces cerevisiae strain DAUFPE-1012 were studied during a 24 h exposure to 220 mT steady magnetic fields (SMF) at 23 +/- 1 degrees C, produced by NdFeB rod magnets. The magnets were attached diametrically opposed (N to S) to a cylindrical tube reactor. The biomass growth in the reactor culture media (yeast extract + glucose 2%) during 24 h was monitored by measurements of optical density, which was correlated to cell dry weight. Ethanol concentration and glucose level were measured every 2 h. The pH of the culture media was maintained between 4 and 5. As a result, biomass (g/L) increased 2.5-fold and ethanol concentration 3.4-fold in magnetized cultures (n = 8) as compared with SMF nonexposed cultures (n = 8). Glucose consumption was higher in magnetized cultures, which correlated to the ethanol yield. PMID: 14763869 [PubMed - indexed for MEDLINE] Preliminary results on the non-thermal effects of 200-350 GHz radiation on the growth rate of S. cerevisiae cells in microcolonies. Hadjiloucas S, Chahal MS, Bowen JW. Department of Cybernetics, The University of Reading, Whiteknights, RG6 6AY, Berkshire, UK. We report preliminary results from studies of biological effects induced by non-thermal levels of non-ionizing electromagnetic radiation. Exponentially growing Saccharomyces cerevisiae yeast cells grown on dry media were exposed to electromagnetic fields in the 200-350 GHz frequency range at low power density to observe possible non-thermal effects on the microcolony growth. Exposure to the electromagnetic field was conducted over 2.5 h. The data from exposure and control experiments were grouped into either large-, medium- or small-sized microcolonies to assist in the accurate assessment of growth. The three groups showed significant differences in growth between exposed and control microcolonies. A statistically significant enhanced growth rate was observed at 341 GHz. Growth rate was assessed every 30 min via time-lapse photography. Possible interaction mechanisms are discussed, taking into account Frohlich's hypothesis. PMID: 12452574 [PubMed - indexed for MEDLINE] Low-level, magnetic-field-induced growth modification of Bacillus subtilis. Ramon C, JT, MR. Department of Physical Science, Institute of Applied Physiology and Medicine, Seattle, Washington 98122. Experimental studies showed an increase in the growth of Bacillus subtilis mutant strain FJ7 above controls by exposing the bacterial culture to 800-Hz or 1-KHz magnetic fields with a 2-s-on/2-s-off period. The magnetic field strength was between 0.8 and 2.5 mT. Light microscopy and scanning electron microscopy demonstrated the morphology of controls to grow in a macrofiber of right-handed helix formation. In contrast, the field- exposed group showed little to no cohesion; the cells appeared to be homogeneously distributed throughout the sample. These results suggest that growth patterns of Bacillus subtilis can be altered as a result of magnetic-field-induced effects. PMID: 3117065 [PubMed - indexed for MEDLINE] Stability improvement of immobilized Candida antarctica lipase B in an organic medium under microwave radiation. Rejasse B, Lamare S, Legoy MD, Besson T. Laboratoire de Biotechnologies et Chimie Bioorganique, CNRS FRE-2766, UFR Sciences Fondamentales et Sciences pour l'Ingenieur, Batiment Marie Curie, Universite de la Rochelle, F-17042, La Rochelle cedex 1, France. The influence of microwave heating on the stability of immobilized Candida antarctica lipase B was studied at 100 degrees in an organic medium. The microwave radiation was carried out before enzymatic reaction (storage conditions) or during the enzymatic catalysis (use conditions). In both cases, enzymatic stability was higher under microwave heating than under conventional thermal heating, in strictly identical operating conditions. Furthermore, the gain of enzymatic stability under microwave heating appears to be higher in a more polar solvent, which interacts strongly with the microwave field. Our results suggest that microwave radiation has an effect, not related to temperature, on the process of enzymatic inactivation. PMID: 15034633 [PubMed - indexed for MEDLINE] Quote Link to comment Share on other sites More sharing options...
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