Guest guest Posted September 5, 2006 Report Share Posted September 5, 2006 Hi, Jim. jschm111@... wrote: Seeing it at > 2, I'm assuming > that's as high as it gets. Unfortunately, there is no direct way to attack > calpain as far as I know. > > Jim ***Glutathione repletion and fixing methylation blocks will do this. Low glutathione is what disinhibits calpain and in consequence activates your RnaseL enzyme system. ***My RnaseL levels were high abnormal and I had the famous chronic flu-like feeling to go along. Nondenatured whey use to replete glutathione decisively took these symptoms away in my case. Quote Link to comment Share on other sites More sharing options...
Guest guest Posted September 6, 2006 Report Share Posted September 6, 2006 Hi Jim In my daughter`s case it is elastase, not calpain, that seems to be the problem - so no experience with calpain. But I remember you have posted about increased Cadmium. I saw that Cadmium can activate calpain: " Apoptosis Induced by Cadmium in Human Lymphoma U937 Cells through Ca2-calpain and Caspase-Mitochondria- dependent Pathways " http://www.jbc.org/cgi/content/full/275/50/39702 So maybe if it is possible to decrease the Cadmium level, that would help with the Calpain problem? ne > > Got my calpain results from Red Labs: > > >2.0 (normal: 0.18-0.27) > > My elastase was at the upper normal range, RNase L activity and low-weight > RNase L both quite high. Looks like calpain is the culprit in cleaving RNase L. > > > Has anyone else had the calpain test done? Seeing it at > 2, I'm assuming > that's as high as it gets. Unfortunately, there is no direct way to attack > calpain as far as I know. > > Jim > > > Quote Link to comment Share on other sites More sharing options...
Guest guest Posted September 6, 2006 Report Share Posted September 6, 2006 Hi, ne. Good find. Cadmium like mercury and other heavy metals can savage the enzymes for making and using glutathione(GSH). In turn, as GSH goes low this disinibits calpain to action, which action upregulates RnaseL and as far as I know potentially elastase too. So yes, getting rid of cadmium, if present, would help as well as dealing with other things that lower GSH like methylation blocks. The issue of GSH status supercedes the downstream issues of calpain, RnaseL and elastase in CFS. " s_fibaek " <s_fibaek@...> wrote: > > Hi Jim > > In my daughter`s case it is elastase, not calpain, that seems to be > the problem - so no experience with calpain. > > But I remember you have posted about increased Cadmium. I saw that > Cadmium can activate calpain: > > " Apoptosis Induced by Cadmium in Human Lymphoma U937 Cells through > Ca2-calpain and Caspase-Mitochondria- dependent Pathways " > http://www.jbc.org/cgi/content/full/275/50/39702 > > So maybe if it is possible to decrease the Cadmium level, that would > help with the Calpain problem? > > ne Quote Link to comment Share on other sites More sharing options...
Guest guest Posted September 6, 2006 Report Share Posted September 6, 2006 Hi Jim, Just out of interest, when did you have your blood taken? I had mine taken three weeks ago but I still haven't got the RNase-L test results back yet. Everytime I phone Red Labs I seem to get a different story. I didn't have calpain tested, just the elastase and the result was 2276 compared with a normal upper result of <150 Many thanks Mark > > Got my calpain results from Red Labs: > > >2.0 (normal: 0.18-0.27) > > My elastase was at the upper normal range, RNase L activity and low-weight > RNase L both quite high. Looks like calpain is the culprit in cleaving RNase L. > > > Has anyone else had the calpain test done? Seeing it at > 2, I'm assuming > that's as high as it gets. Unfortunately, there is no direct way to attack > calpain as far as I know. > > Jim > > > Quote Link to comment Share on other sites More sharing options...
Guest guest Posted September 6, 2006 Report Share Posted September 6, 2006 Hi , I had trace Cadmium found in the Biolab translocator test. Do you know if cadmium also affects elastase? I haven't had calpain levels checked but my elastase was 15 times higher than the normal upper limit and my RNase-L activity is higher than normal. I have also had a lowish result for glutathione peroxidase. Thanks Mark > > > > Hi Jim > > > > In my daughter`s case it is elastase, not calpain, that seems to be > > the problem - so no experience with calpain. > > > > But I remember you have posted about increased Cadmium. I saw that > > Cadmium can activate calpain: > > > > " Apoptosis Induced by Cadmium in Human Lymphoma U937 Cells through > > Ca2-calpain and Caspase-Mitochondria- dependent Pathways " > > http://www.jbc.org/cgi/content/full/275/50/39702 > > > > So maybe if it is possible to decrease the Cadmium level, that would > > help with the Calpain problem? > > > > ne > Quote Link to comment Share on other sites More sharing options...
Guest guest Posted September 6, 2006 Report Share Posted September 6, 2006 , I tried whey some years back to no avail. Also my gluatthione fell in the normal range (towards the bottom). But maybe the methylation cycle could be involved. Anyway, may try the whey again. Thanks, Jim Quote Link to comment Share on other sites More sharing options...
Guest guest Posted September 6, 2006 Report Share Posted September 6, 2006 Mark, I sent mine to Red Labs in the US a few months ago. The calpain takes longer than normal. The RNase L should only take a week I think. I will say that the US lab is not so hot on customer relations. I've called them many times to track down results. I checked and my elastase was just above normal, but nothing like yours and others. Jim Quote Link to comment Share on other sites More sharing options...
Guest guest Posted September 6, 2006 Report Share Posted September 6, 2006 ne, Thanks for the cadmium/calpain link! I'll be contacting the author and hope he speaks english. I know I have high cell death, so perhaps cadmium and calpain are part of it. I don't know if my cadmium is high enough to be a big factor but will check it out. Maybe try to confirm levels some other way. Jim Quote Link to comment Share on other sites More sharing options...
Guest guest Posted September 6, 2006 Report Share Posted September 6, 2006 Hi Jim There is also another cause of calpain activity that I have seen mentioned; Infection with reovirus. " Reovirus-Induced Apoptosis Is Preceded by Increased Cellular Calpain Activity and Is Blocked by Calpain Inhibitors " http://tinyurl.com/f34bq I don`t know of there are differences between the reoviruses in their ability to induce apoptosis, and I also don`t know if some of them might be able to establish a persistent infection. Two reoviruses are: Colorado tick fever virus, and rotavirus. There was another link at pubmed concerning this in, which they said that (among others) Minocycline might help to reduce the apoptosis: " Mechanisms of reovirus-induced cell death and tissue injury: role of apoptosis and virus-induced perturbation of host-cell signaling and transcription factor activation. " http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search & DB=pubmed ne > > ne, > > Thanks for the cadmium/calpain link! I'll be contacting the author and hope > he speaks english. I know I have high cell death, so perhaps cadmium and > calpain are part of it. I don't know if my cadmium is high enough to be a big > factor but will check it out. Maybe try to confirm levels some other way. > > Jim > > > Quote Link to comment Share on other sites More sharing options...
Guest guest Posted September 6, 2006 Report Share Posted September 6, 2006 Hi, Mark. " hewgim " <mark_hewgill@...> wrote: > > Hi , > > I had trace Cadmium found in the Biolab translocator test. Do you > know if cadmium also affects elastase? I haven't had calpain levels > checked but my elastase was 15 times higher than the normal upper > limit and my RNase-L activity is higher than normal. I have also had > a lowish result for glutathione peroxidase. > > Thanks > > Mark ***Yes, cadmium is a toxic metal like mercury that savages glutathione producing enzymes, so this fact says it does effect elastase. Also, don't bother to check calpain, save your money, as high RnaseL and lowish GPx tell us it got activated. > <davidhall@> wrote: The issue of GSH status supercedes > the downstream issues of calpain, RnaseL and elastase in CFS. Quote Link to comment Share on other sites More sharing options...
Guest guest Posted September 6, 2006 Report Share Posted September 6, 2006 Hi, Jim. jschm111@... wrote: > > , > > I tried whey some years back to no avail. ***Me too. But now I think the problem for some like me was the type of whey we were using. ***Turns out NONdenatured wheys such as RenewPro and The True Whey are far superior for sulfur tolerant PWCs in building glutathione compared to using denatured or undenatured wheys. Also my gluatthione fell in the > normal range (towards the bottom). But maybe the methylation cycle could be involved. ***Could be. Anyway, may try the whey again. > > Thanks, > > Jim *** Quote Link to comment Share on other sites More sharing options...
Guest guest Posted September 7, 2006 Report Share Posted September 7, 2006 What is GPx ? Re: Calpain Result Hi, Mark. " hewgim " <mark_hewgill@...> wrote: > > Hi , > > I had trace Cadmium found in the Biolab translocator test. Do you > know if cadmium also affects elastase? I haven't had calpain levels > checked but my elastase was 15 times higher than the normal upper > limit and my RNase-L activity is higher than normal. I have also had > a lowish result for glutathione peroxidase. > > Thanks > > Mark ***Yes, cadmium is a toxic metal like mercury that savages glutathione producing enzymes, so this fact says it does effect elastase. Also, don't bother to check calpain, save your money, as high RnaseL and lowish GPx tell us it got activated. > <davidhall@> wrote: The issue of GSH status supercedes > the downstream issues of calpain, RnaseL and elastase in CFS. Quote Link to comment Share on other sites More sharing options...
Guest guest Posted September 7, 2006 Report Share Posted September 7, 2006 Hi, . > What is GPx ? ***Glutathione Peroxidase(GPx). From: davidhall2020 > ***Yes, cadmium is a toxic metal like mercury that savages glutathione producing enzymes, so this fact says it does effect elastase. Also, don't bother to check calpain, save your money, as high RnaseL and lowish GPx tell us it got activated. > > > > > > Quote Link to comment Share on other sites More sharing options...
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