Effect of thimerosal, a preservative in vaccines, on intracellular Ca(2+) concen

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1: Toxicology. 2004 Jan 15;195(1):77-84.

Effect of thimerosal, a preservative in vaccines, on intracellular Ca(2+)

concentration of rat cerebellar neurons.

Ueha-Ishibashi T, Oyama Y, Nakao H, Umebayashi C, Nishizaki Y, Tatsuishi T,

Iwase K, Murao K, Seo H.

Laboratory of Cellular Signaling, Faculty of Integrated Arts and Sciences,

The University of Tokushima, 770-8502, Tokushima, Japan

The effect of thimerosal, an organomercurial preservative in vaccines, on

cerebellar neurons dissociated from 2-week-old rats was compared with those

of methylmercury using a flow cytometer with appropriate fluorescent dyes.

Thimerosal and methylmercury at concentrations ranging from 0.3 to 10microM

increased the intracellular concentration of Ca(2+) ([Ca(2+)]i) in a

concentration-dependent manner. The potency of 10microM thimerosal to

increase the [Ca(2+)]i was less than that of 10microM methylmercury. Their

effects on the [Ca(2+)]i were greatly attenuated, but not completely

suppressed, under external Ca(2+)-free condition, suggesting a possibility

that both agents increase membrane Ca(2+) permeability and release Ca(2+)

from intracellular calcium stores. The effect of 10microM thimerosal was

not affected by simultaneous application of 30microM L-cysteine whereas

that of 10microM methylmercury was significantly suppressed. The potency of

thimerosal was similar to that of methylmercury in the presence of

L-cysteine. Both agents at 1microM or more similarly decreased the cellular

content of glutathione in a concentration-dependent manner, suggesting an

increase in oxidative stress. Results indicate that thimerosal exerts some

cytotoxic actions on cerebellar granule neurons dissociated from 2-week-old

rats and its potency is almost similar to that of methylmercury.

PMID: 14698570 [PubMed - as supplied by publisher]

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Sheri Nakken, R.N., MA, Classical Homeopath

Vaccination Information & Choice Network, Nevada City CA & Wales UK

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