Re: Igenex Results - Help intepreting

[Guest guest]

What lab did you send to? According to CDC and Igenex I think these

would indicate a " positive " for Lymes. If you sent to Labcorp or Quest

they interpret differently. Is your doc an LLMD? Look up Lyme bands on

google and you will find information about how to interpret the

results. IgeneX may also have this info. on their web site.

B.

>

> My IGG Results showed nothing except:

>

> **31kDa ++

> **39kDa IND

> **41kDa+++

>

> IGM

>

> **23-25kDaIND

> 28 kDa +

> **31kDa++

> **34kDa+

> **39kDa IND

> **41kDa++

> 45kDa+

> 58kDA++

>

> Doc says this isn't Lyme but will be treating me for

> another " sprirocet " . Does this make sense?

>

> Thanks

>

[Guest guest]

PS:

I may be wrong in my understanding but I think you only need to have 1

or 2 positive for it to be delcared Lymes and you have many more than

that!

> >

> > My IGG Results showed nothing except:

> >

>

>

> > **31kDa ++

> > **39kDa IND

> > **41kDa+++

> >

> > IGM

> >

> > **23-25kDaIND

> > 28 kDa +

> > **31kDa++

> > **34kDa+

> > **39kDa IND

> > **41kDa++

> > 45kDa+

> > 58kDA++

> >

> > Doc says this isn't Lyme but will be treating me for

> > another " sprirocet " . Does this make sense?

> >

> > Thanks

> >

>

[Guest guest]

Oms D, which is band 28 is specific for Lyme. (This protein is

usually called Osp D)

It's much more common in the european species.

Present in the northamericam species only 24% of the time.

No question you have Lyme. 9Band 31 and 34 are also highly specific

for Lyme.

I can't imagine any Lyme Dr. having a question about these results

The following is a discussion I had with someone else oin this

subject on Lyme Net: (here's the link if you want it) but I've copied

most of it below.

http://flash.lymenet.org/ubb/ultimatebb.php?ubb=get_topic;f=1;t=042187

Barb

REFERENCE 1 SIGNIFICANCE of BAND 28 kDa Osp D

J Bacteriol. 1994 Aug;176(15):4572-82

Analysis of the distribution and molecular heterogeneity of the ospD

gene among the Lyme disease spirochetes: evidence for lateral gene

exchange.

Marconi RT, s DS, Landry RK, Garon CF.

Laboratory of Vectors and Pathogens, Rocky Mountain Laboratories,

National Institute of Allergy and Infectious Diseases, Hamilton,

Montana 59840.

Analysis of the ospD gene has revealed that this gene is not

universal among Lyme disease spirochete isolates. The gene was found

to be carried by 90, 50, and 24% of the Borrelia garinii, B. afzelii,

and B. burgdorferi isolates tested. Size variability in the ospD-

encoding plasmid was also observed. Sequence analysis has

demonstrated the presence of various numbers of a 17-bp repeated

sequence in the upstream control (promoter) region of the gene. In

addition, a region within the coding sequence where various

insertions, deletions, and direct repeats occur was identified. ospD

gene sequences from 31 different isolates were determined and

utilized in pairwise sequence comparisons and construction of a gene

tree. These analyses suggest that the ospD gene was the target of

several recombinational events and that the gene was recently

acquired by Lyme disease spirochetes and laterally transferred

between species.

PMID: 7913928 [PubMed -

REFERENCE 2

Infect Immun. 2004 Nov;72(11):6279-86.

Extracellular secretion of the Borrelia burgdorferi Oms28 porin and

Bgp, a glycosaminoglycan binding protein.

Cluss RG, Silverman DA, Stafford TR.

Department of Chemistry and Biochemistry, Middlebury College, VT

05753, USA.

----------------------------------------------------------------------

----------

REFERENCE 3

I meant to add - it might be of interest when choosing the right

antibiotic-

since Osp D is a penicillin binding protein-

You might want to talk to your Doc about using high dose Amoxycillin

since you're expressing antibodies to 28 kDa.

Barb

Microb Pathog. 1995 Oct;19(4):257-72.

Chemiluminescent analysis of Borrelia burgdorferi penicillin-binding

proteins using ampicillin conjugated to digoxigenin.

Norgard MV, Baker SI, Radolf JD.

Department of Microbiology, University of Texas Southwestern Medical

Center, Dallas 75235, USA.

Knowledge of the penicillin-binding proteins (PBPs) of Borrelia

burgdorferi is important for understanding both the targets of beta-

lactams used therapeutically for Lyme borreliosis and the complex

membrane biology of the distinctive spirochetal pathogen which causes

Lyme disease.

In this study, the PBPs of a number of B. burgdorferi strains and

variants were examined using a rapid and sensitive chemiluminescent

assay which employs ampicillin conjugated to digoxigenin (dig-amp).

The minimum inhibitory concentration of dig-amp for B. burgdorferi

high-passage strain B31 (0.012 micrograms/ml) was essentially no

different from that of free ampicillin (0.025 micrograms/ml). Dig-amp

bound specifically to B. burgdorferi B31 PBPs with molecular masses

of 92, 80, 65, 46, 40, 34, 31, 29, 22, 20 and 13 kDa; the 31 kDa and

34 kDa PBPs were proven to be OspA and OspB, respectively. All of the

borrelial PBPs were present in the cytoplasmic membrane fraction of

B. burgdorferi, findings consistent with their activities as PBPs but

inconsistent with OspA and OspB as surface-exposed outer membrane

lipoproteins. Furthermore, among the PBP profiles of other high- and

low-passage variants of B. burgdorferi strains Sh-2-82, HB19, and

N40, which differed somewhat from one another,

OspD (28 kDa) but not OspC (22-25 kDa) also was strongly implicated

as a PBP;

however, OspC possessed a gel mobility easily misconstrued as that of

a 26 kDa PBP often expressed reciprocally with OspB. The

ramifications of classifying OspA, OspB, and OspD as PBPs are

discussed. While the current inability to genetically manipulate B.

burgdorferi hinders determining which of the borrelial PBPs are

essential for spirochetal viability (i.e., are the lethal targets of

beta-lactams), a priori knowledge of the borrelial PBPs will

facilitate the production and purification of recombinant derivatives

whose activities can be assessed further in vitro.

PMID: 8825913 [PubMed - indexed for MEDLINE]

REFERENCE 4 ABSTRACT:

And here's a paper by Alan B before he went over to the Dark Side

showing that OspD's expression is a virulence factor.

(and the meaning of virluence is the capacity of a microorganism to

cause disease).

So, while the conventional Drs. may question your blot as to whether

it's negative or positive for Lyme - by research standards, you are

positive.

Now you have entered the Twilight Zone between Research and

Convention and will have to decide what you beleive.

Infect Immun. 1992 Nov;60(11):4662-72.

Low-passage-associated proteins of Borrelia burgdorferi B31:

characterization and molecular cloning of OspD, a surface-exposed,

plasmid-encoded lipoprotein.

Norris SJ, CJ, Howell JK, Barbour AG.

Department of Pathology and Laboratory Medicine, University of Texas

Medical School, Houston 77225.

Borrelia burgdorferi, the causative agent of Lyme disease, loses its

ability to infect and cause disease in mammalian hosts after repeated

in vitro passage.

To identify proteins preferentially expressed by the low-passage

strain and thus representing potential virulence factors,

the polypeptide profiles of virulent, low-passage

and nonvirulent, high-passage forms of B. burgdorferi B31 were

compared by nonequilibrium pH gradient two-dimensional gel

electrophoresis.

Four low-passage-associated proteins with relative molecular masses (M

®s) of 35,000, 28,000, 24,000, and 20,000 were identified. Of

these, the 28- and 35-kDa polypeptides were not expressed in

detectable quantities in the high-passage B31 strain, whereas the 24-

and 20-kDa proteins were present in reduced quantities. All four of

these proteins were lipoproteins, as determined by labelling with [3H]

palmitate.

The abundant 28-kDa component, called outer surface protein D (OspD),

is surface exposed on the basis of its proteolysis during treatment

of intact organisms with proteinase K. The ospD gene is located on a

38-kb linear plasmid present in seven of nine low-passage strains of

B. burgdorferi examined but absent in most high-passage, nonvirulent

strains tested.

Molecular cloning and sequence analysis of the ospD gene locus

revealed an open reading frame encoding a 28,436-Da polypeptide with

a putative signal peptidase II leader sequence. An unusual feature of

the region upstream of the gene was the presence of seven contiguous,

direct repeats of a 17-bp sequence that includes consensus -35 and -

10 transcription initiation signals; however, only one transcription

initiation site was active as determined by primer extension

analysis. Further study of these and other polypeptides associated

with low-passage strains may lead to identification of B. burgdorferi

gene products required for infection and pathogenesis in mammalian

hosts.

PMID: 1398980 [PubMed - indexed for MEDLINE]

>

> My IGG Results showed nothing except:

>

> **31kDa ++

> **39kDa IND

> **41kDa+++

>

> IGM

>

> **23-25kDaIND

> 28 kDa +

> **31kDa++

> **34kDa+

> **39kDa IND

> **41kDa++

> 45kDa+

> 58kDA++

>

> Doc says this isn't Lyme but will be treating me for

> another " sprirocet " . Does this make sense?

>

> Thanks

>

[Guest guest]

I've read the other replies to you. Your labs look as positive as

mine did from IgeneX or moreso.

Your doctor may be making a " point " of distinguishing your

spirochette from some other strain. So...my question would be what

treatment is he or she prescribing. Barb Peck made an interesting

point that your particular infection may respond to a penicillin

better than most.

Is your doctor willing to work with you to determine what

antibiotics, in fact, work for you? I think the treatment is, at this

point, mostly art and not science.

a Carnes

>

> My IGG Results showed nothing except:

>

> **31kDa ++

> **39kDa IND

> **41kDa+++

>

> IGM

>

> **23-25kDaIND

> 28 kDa +

> **31kDa++

> **34kDa+

> **39kDa IND

> **41kDa++

> 45kDa+

> 58kDA++

>

> Doc says this isn't Lyme but will be treating me for

> another " sprirocet " . Does this make sense?

>

> Thanks

>

[Guest guest]

She will be treating me with Doxycycline and anti virals (IV's) for

now.

>

> I've read the other replies to you. Your labs look as positive as

> mine did from IgeneX or moreso.

>

> Your doctor may be making a " point " of distinguishing your

> spirochette from some other strain. So...my question would be what

> treatment is he or she prescribing. Barb Peck made an interesting

> point that your particular infection may respond to a penicillin

> better than most.

>

> Is your doctor willing to work with you to determine what

> antibiotics, in fact, work for you? I think the treatment is, at

this

> point, mostly art and not science.

>

> a Carnes

>

>

> >

> > My IGG Results showed nothing except:

> >

> > **31kDa ++

> > **39kDa IND

> > **41kDa+++

> >

> > IGM

> >

> > **23-25kDaIND

> > 28 kDa +

> > **31kDa++

> > **34kDa+

> > **39kDa IND

> > **41kDa++

> > 45kDa+

> > 58kDA++

> >

> > Doc says this isn't Lyme but will be treating me for

> > another " sprirocet " . Does this make sense?

> >

> > Thanks

> >

>

[Guest guest]

Beg her to use minocycline rather than doxycycline. Doxy has never

worked for anyone longterm. Then she can add Zithromax for months to

years. Let me just say that the Gulf War vets infected with

mycoplasma did terrible on doxy. It just doesn't work. Minocycline is

what the Roadback people are using - low dose - for a long time. But

Schlifer will know this. You will be okay with her as long as you can

keep the bill straight!!!

a

>

> She will be treating me with Doxycycline and anti virals (IV's) for

> now.

>

>

> >

> > I've read the other replies to you. Your labs look as positive as

> > mine did from IgeneX or moreso.

> >

> > Your doctor may be making a " point " of distinguishing your

> > spirochette from some other strain. So...my question would be

what

> > treatment is he or she prescribing. Barb Peck made an interesting

> > point that your particular infection may respond to a penicillin

> > better than most.

> >

> > Is your doctor willing to work with you to determine what

> > antibiotics, in fact, work for you? I think the treatment is, at

> this

> > point, mostly art and not science.

> >

> > a Carnes

> >

> >

> > >

> > > My IGG Results showed nothing except:

> > >

> > > **31kDa ++

> > > **39kDa IND

> > > **41kDa+++

> > >

> > > IGM

> > >

> > > **23-25kDaIND

> > > 28 kDa +

> > > **31kDa++

> > > **34kDa+

> > > **39kDa IND

> > > **41kDa++

> > > 45kDa+

> > > 58kDA++

> > >

> > > Doc says this isn't Lyme but will be treating me for

> > > another " sprirocet " . Does this make sense?

> > >

> > > Thanks

> > >

> >

>

[Guest guest]

I just wonder why she isn't calling it Lymes as your test is glaring

for it. She told you that you didn't have Lymes, but is treating a

spirochete. But Lymes IS a spirochete.

Ballady

> >

> > I've read the other replies to you. Your labs look as positive as

> > mine did from IgeneX or moreso.

> >

> > Your doctor may be making a " point " of distinguishing your

> > spirochette from some other strain. So...my question would be what

> > treatment is he or she prescribing. Barb Peck made an interesting

> > point that your particular infection may respond to a penicillin

> > better than most.

> >

> > Is your doctor willing to work with you to determine what

> > antibiotics, in fact, work for you? I think the treatment is, at

> this

> > point, mostly art and not science.

> >

> > a Carnes

> >

> >

> > >

> > > My IGG Results showed nothing except:

> > >

> > > **31kDa ++

> > > **39kDa IND

> > > **41kDa+++

> > >

> > > IGM

> > >

> > > **23-25kDaIND

> > > 28 kDa +

> > > **31kDa++

> > > **34kDa+

> > > **39kDa IND

> > > **41kDa++

> > > 45kDa+

> > > 58kDA++

> > >

> > > Doc says this isn't Lyme but will be treating me for

> > > another " sprirocet " . Does this make sense?

> > >

> > > Thanks

> > >

> >

>

[Guest guest]

She thinks it's a different germ - not technically Lyme.

> > >

> > > I've read the other replies to you. Your labs look as positive

as

> > > mine did from IgeneX or moreso.

> > >

> > > Your doctor may be making a " point " of distinguishing your

> > > spirochette from some other strain. So...my question would be

what

> > > treatment is he or she prescribing. Barb Peck made an

interesting

> > > point that your particular infection may respond to a

penicillin

> > > better than most.

> > >

> > > Is your doctor willing to work with you to determine what

> > > antibiotics, in fact, work for you? I think the treatment is,

at

> > this

> > > point, mostly art and not science.

> > >

> > > a Carnes

> > >

> > >

> > > >

> > > > My IGG Results showed nothing except:

> > > >

> > > > **31kDa ++

> > > > **39kDa IND

> > > > **41kDa+++

> > > >

> > > > IGM

> > > >

> > > > **23-25kDaIND

> > > > 28 kDa +

> > > > **31kDa++

> > > > **34kDa+

> > > > **39kDa IND

> > > > **41kDa++

> > > > 45kDa+

> > > > 58kDA++

> > > >

> > > > Doc says this isn't Lyme but will be treating me for

> > > > another " sprirocet " . Does this make sense?

> > > >

> > > > Thanks

> > > >

> > >

> >

>

[Guest guest]

> > >

> > > I've read the other replies to you. Your labs look as positive

as

> > > mine did from IgeneX or moreso.

> > >

> > > Your doctor may be making a " point " of distinguishing your

> > > spirochette from some other strain. So...my question would be

what

> > > treatment is he or she prescribing. Barb Peck made an

interesting

> > > point that your particular infection may respond to a

penicillin

> > > better than most.

> > >

> > > Is your doctor willing to work with you to determine what

> > > antibiotics, in fact, work for you? I think the treatment is,

at

> > this

> > > point, mostly art and not science.

> > >

> > > a Carnes

> > >

> > >

> > > >

> > > > My IGG Results showed nothing except:

> > > >

> > > > **31kDa ++

> > > > **39kDa IND

> > > > **41kDa+++

> > > >

> > > > IGM

> > > >

> > > > **23-25kDaIND

> > > > 28 kDa +

> > > > **31kDa++

> > > > **34kDa+

> > > > **39kDa IND

> > > > **41kDa++

> > > > 45kDa+

> > > > 58kDA++

> > > >

> > > > Doc says this isn't Lyme but will be treating me for

> > > > another " sprirocet " . Does this make sense?

> > > >

> > > > Thanks

> > > >

> > >

> >

>

[Guest guest]

> > >

> > > I've read the other replies to you. Your labs look as positive

as

> > > mine did from IgeneX or moreso.

> > >

> > > Your doctor may be making a " point " of distinguishing your

> > > spirochette from some other strain. So...my question would be

what

> > > treatment is he or she prescribing. Barb Peck made an

interesting

> > > point that your particular infection may respond to a

penicillin

> > > better than most.

> > >

> > > Is your doctor willing to work with you to determine what

> > > antibiotics, in fact, work for you? I think the treatment is,

at

> > this

> > > point, mostly art and not science.

> > >

> > > a Carnes

> > >

> > >

> > > >

> > > > My IGG Results showed nothing except:

> > > >

> > > > **31kDa ++

> > > > **39kDa IND

> > > > **41kDa+++

> > > >

> > > > IGM

> > > >

> > > > **23-25kDaIND

> > > > 28 kDa +

> > > > **31kDa++

> > > > **34kDa+

> > > > **39kDa IND

> > > > **41kDa++

> > > > 45kDa+

> > > > 58kDA++

> > > >

> > > > Doc says this isn't Lyme but will be treating me for

> > > > another " sprirocet " . Does this make sense?

> > > >

> > > > Thanks

> > > >

> > >

> >

>

[Guest guest]

Doxycycline is a poor choice for lyme. Barb's post was brilliant and

she is right. You would do better on a penicillin--either high dose

amoxicillin (at least 4 grams a day) or bicillin shots. Borrelia is

pretty susceptible to penicillin and it gets killed by it. Doxycycline

will inhibit it but one reason I'm still suffering is that was all

they would give me--doxycycline. All it did, for 6 weeks, is inhibit it.

> > > >

> > > > I've read the other replies to you. Your labs look as positive

> as

> > > > mine did from IgeneX or moreso.

> > > >

> > > > Your doctor may be making a " point " of distinguishing your

> > > > spirochette from some other strain. So...my question would be

> what

> > > > treatment is he or she prescribing. Barb Peck made an

> interesting

> > > > point that your particular infection may respond to a

> penicillin

> > > > better than most.

> > > >

> > > > Is your doctor willing to work with you to determine what

> > > > antibiotics, in fact, work for you? I think the treatment is,

> at

> > > this

> > > > point, mostly art and not science.

> > > >

> > > > a Carnes

> > > >

> > > >

> > > > >

> > > > > My IGG Results showed nothing except:

> > > > >

> > > > > **31kDa ++

> > > > > **39kDa IND

> > > > > **41kDa+++

> > > > >

> > > > > IGM

> > > > >

> > > > > **23-25kDaIND

> > > > > 28 kDa +

> > > > > **31kDa++

> > > > > **34kDa+

> > > > > **39kDa IND

> > > > > **41kDa++

> > > > > 45kDa+

> > > > > 58kDA++

> > > > >

> > > > > Doc says this isn't Lyme but will be treating me for

> > > > > another " sprirocet " . Does this make sense?

> > > > >

> > > > > Thanks

> > > > >

> > > >

> > >

> >

>

[Guest guest]

That's the old CDC line. Bb sensu strictu, and they leave all kinds of

folks with borrelia out in the cold--Masters is still fighting

them--they call it STARI and act confused and suggest it is much more

self limiting than lyme. It's all borrelia. Some strains may be more

virulent, some may have a greater affinity for the CNS; okay, maybe

this strain didn't start in Old Lyme CT but big deal.

> > > >

> > > > I've read the other replies to you. Your labs look as positive

> as

> > > > mine did from IgeneX or moreso.

> > > >

> > > > Your doctor may be making a " point " of distinguishing your

> > > > spirochette from some other strain. So...my question would be

> what

> > > > treatment is he or she prescribing. Barb Peck made an

> interesting

> > > > point that your particular infection may respond to a

> penicillin

> > > > better than most.

> > > >

> > > > Is your doctor willing to work with you to determine what

> > > > antibiotics, in fact, work for you? I think the treatment is,

> at

> > > this

> > > > point, mostly art and not science.

> > > >

> > > > a Carnes

> > > >

> > > >

> > > > >

> > > > > My IGG Results showed nothing except:

> > > > >

> > > > > **31kDa ++

> > > > > **39kDa IND

> > > > > **41kDa+++

> > > > >

> > > > > IGM

> > > > >

> > > > > **23-25kDaIND

> > > > > 28 kDa +

> > > > > **31kDa++

> > > > > **34kDa+

> > > > > **39kDa IND

> > > > > **41kDa++

> > > > > 45kDa+

> > > > > 58kDA++

> > > > >

> > > > > Doc says this isn't Lyme but will be treating me for

> > > > > another " sprirocet " . Does this make sense?

> > > > >

> > > > > Thanks

> > > > >

> > > >

> > >

> >

>

[Guest guest]

You might want to check this website for what bands are considered important for

diagnosing the different Borrelias in Europe.

Note that band 58 (IgG) is considered specific for Borrelia afzelii (PKo strain)

Nelly

http://pollux.mpk.med.uni-muenchen.de/alpha1/nrz-borrelia/miq-lyme/frame-miq-tab\

le9.html

Table 9. Interpretation rules for the immunoblot

Whole-cell lysate blot

For use of PKo strain (B. afzelii):

IgG: positive if >=2 bands of the following are present:

p83/100, p58, p43, p39, p30, OspC, p21, Osp17, p14

IgM: positive if >=1 band of the following is/are present:

p41 (distinct), p39, OspC, Osp17

Recombinant antigen blot

IgG: positive if >=2 bands of the following are present:

p83/100, p58, p39, OspC, p41int*, Osp17

IgM: positive if >=2 band of the following is/are present:

p39, OspC, p41int, Osp17

or OspC alone and distinct

* p41int is the central variable region of the flagellin gene with a molecular

weight of 14000 Da.

In the IgM immunoblot, the detectable immune response is restricted to only a

few bands. For this reason the presence of only one band is, under certain

circumstances, regarded as a positive result. In some instances band intensity

must also be taken into account (cf. Table 9).

Regarding the general requirements for technical implementation of the

immunoblot, the reader is referred to DIN 58967, Part 40.

The following special requirements apply to the development and use of

immunoblots for the detection of antibodies against B. burgdorferi:

The strain used in the whole-cell lysate blot must express the immunorelevant

proteins in culture. By means of monoclonal antibodies or adequate patient sera

it must be shown that p83/100, p39, OspC and, if possible, also Osp 17 are

expressed. The proteins closely adjacent on the blot strip (p60 and p58, p41 and

p39, OspA and p30 as well as OspC and p21) must be clearly distinguishable from

one another. For newly developed tests (e.g. those using other strains as test

antigens) the interpretation criteria must be newly established in comprehensive

studies, by means of defined patient and control serum panels, as exemplified in

American [9, 13] and European studies [22, 24].

Re: Igenex Results - Help intepreting

She thinks it's a different germ - not technically Lyme.

> > > > My IGG Results showed nothing except:

> > > >

> > > > **31kDa ++

> > > > **39kDa IND

> > > > **41kDa+++

> > > >

> > > > IGM

> > > >

> > > > **23-25kDaIND

> > > > 28 kDa +

> > > > **31kDa++

> > > > **34kDa+

> > > > **39kDa IND

> > > > **41kDa++

> > > > 45kDa+

> > > > 58kDA++

> > > >

> > > > Doc says this isn't Lyme but will be treating me for

> > > > another " sprirocet " . Does this make sense?

> > > >

[Guest guest]

Interesting information. Does this apply only to European testing?

Is what I'm reading correct - that even if the appropriate bands are

present, and the determination is " positive, " this could still be a

" false positive? " What I don't understand is how the " false " part is

determined.

Ballady

>

> You might want to check this website for what bands are considered

important for diagnosing the different Borrelias in Europe.

> Note that band 58 (IgG) is considered specific for Borrelia afzelii

(PKo strain)

>

> Nelly

>

>

http://pollux.mpk.med.uni-muenchen.de/alpha1/nrz-borrelia/miq-lyme/frame-miq-tab\

le9.html

> Table 9. Interpretation rules for the immunoblot

>

> Whole-cell lysate blot

>

> For use of PKo strain (B. afzelii):

>

> IgG: positive if >=2 bands of the following are present:

> p83/100, p58, p43, p39, p30, OspC, p21, Osp17, p14

>

> IgM: positive if >=1 band of the following is/are present:

> p41 (distinct), p39, OspC, Osp17

>

> Recombinant antigen blot

>

> IgG: positive if >=2 bands of the following are present:

> p83/100, p58, p39, OspC, p41int*, Osp17

>

> IgM: positive if >=2 band of the following is/are present:

> p39, OspC, p41int, Osp17

> or OspC alone and distinct

> * p41int is the central variable region of the flagellin gene with a

molecular weight of 14000 Da.

>

> In the IgM immunoblot, the detectable immune response is restricted

to only a few bands. For this reason the presence of only one band is,

under certain circumstances, regarded as a positive result. In some

instances band intensity must also be taken into account (cf. Table 9).

>

> Regarding the general requirements for technical implementation of

the immunoblot, the reader is referred to DIN 58967, Part 40.

>

> The following special requirements apply to the development and use

of immunoblots for the detection of antibodies against B. burgdorferi:

>

> The strain used in the whole-cell lysate blot must express the

immunorelevant proteins in culture. By means of monoclonal antibodies

or adequate patient sera it must be shown that p83/100, p39, OspC and,

if possible, also Osp 17 are expressed. The proteins closely adjacent

on the blot strip (p60 and p58, p41 and p39, OspA and p30 as well as

OspC and p21) must be clearly distinguishable from one another. For

newly developed tests (e.g. those using other strains as test

antigens) the interpretation criteria must be newly established in

comprehensive studies, by means of defined patient and control serum

panels, as exemplified in American [9, 13] and European studies [22, 24].

>

>

> Re: Igenex Results - Help intepreting

>

>

> She thinks it's a different germ - not technically Lyme.

>

> > > > > My IGG Results showed nothing except:

> > > > >

> > > > > **31kDa ++

> > > > > **39kDa IND

> > > > > **41kDa+++

> > > > >

> > > > > IGM

> > > > >

> > > > > **23-25kDaIND

> > > > > 28 kDa +

> > > > > **31kDa++

> > > > > **34kDa+

> > > > > **39kDa IND

> > > > > **41kDa++

> > > > > 45kDa+

> > > > > 58kDA++

> > > > >

> > > > > Doc says this isn't Lyme but will be treating me for

> > > > > another " sprirocet " . Does this make sense?

> > > > >

>

>

>