various stuff...

[Guest guest]

Melatonin increases activity of complex I and IV in mito's: caught me

attention...

http://www.fasebj.org/cgi/content/full/14/12/1677

Effect of melatonin, NAC, ascorbate, and Trolox on mitochondrial

oxidative complexes

To study any effect of these compounds on the electron transport

chain, we measured the activity of respiratory complexes I, II-III,

and IV in submitochondrial fractions incubated with melatonin (100

nM), NAC (100 ƒÊM), ascorbate (100 ƒÊM), and Trolox (100 ƒÊM). The

results show that only melatonin significantly increased the activity

of complexes I and IV in both brain and liver mitochondria (Fig. 2) .

The activity of complex II-III was not affected by these antioxidants.

http://endo.endojournals.org/cgi/content/abstract/145/1/418

Involvement of Bax Protein in the Prevention of Glucocorticoid-

Induced Thymocytes Apoptosis by Melatonin

On the link between Bcl-2 family proteins and glucocorticoid-induced

apoptosis

Melatonin Relieves the Neural Oxidative Burden that Contributes to

Dementias (ditto the post for high dose melatonin)

http://www.annalsnyas.org/cgi/content/abstract/1035/1/179

A loss of GSH is related to p53--interesting! Will have to do a

search on GSH p53!

http://www.drdebe.com/RECANCOS.htm

http://www.thorne.com/altmedrev/fulltext/glut.html

Are you getting enough L-methionine?

also check out table 2 link in the article--RE: cadmium, mercury

http://www.woodmed.com/Glutathione%202002.htm

Oral L-methionine is a cysteine precursor but can cause nausea and

vomiting, whereas its activated counterpart S-adenosylmethione (SAMe)

is well tolerated. When given i.v. in high doses to cirrhotic

patients, SAMe repleted erythrocyte GSH.

And a bump for ALA:

Concentrations of glutathione, vitamin C and vitamin E, which

decreased during administration of the cataract-inducing chemical,

were restored to levels similar to the controls. a-Lipoic acid also

restored the protein complexes glutathione peroxidase and catalase

Optimal dosage of Vitamin E (a-tocopherol)? I do the mixed

tocopherols

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?

cmd=Retrieve & db=PubMed & list_uids=8937878 & dopt=Abstract

http://www.1whey2health.com/glutathione_heart.htm

And can you believe components of whey lower ACE??

Free download " Glutathione in health and Disease " available here.

Hit the glutathione link on this site: cystine is a cysteine=cysteine

dipeptide and is the prefered form (For the cystine cysteine

controversy)

dang, you can't copy and paste anything of this site... but this was

interesting,

CHECK this out:

Macrophages and Astrocytes prefer cystine for manufacturing GSH while

Lymohocytes and Neurons prefer cysteine.

It seems both would be important...

And another bump for ALA:

http://www.findarticles.com/p/articles/mi_qa3867/is_200309/ai_n9238866

[alpha]-lipoic acid (1,2, dithiolane-3-pentanoic acid) forms a part

of several multienzyme complexes, such as pyruvate dehydrogenase, cc-

ketoglutarate dehydrogenase, and the glycine cleavage system.

Mammalian tissues contain 5-25 nmol/g of lipoic acid8. Four distinct

antioxidant actions of LA have been observed including reactive

oxygen species scavenging activity, capacity to regenerate endogenous

antioxidants such as glutathione, vitamins C and E, metal chelating

activity and repair of oxidized proteins8,9. Beneficial effects of LA

have been documented in metal ion toxicity10 and in carcinogenesis in

experimental animals11.

http://www.patrickholford.com/content.asp?id_Content=928

The problem with glutathione supplementation, however, is that it is

so reactive/protective that it gets used up or oxidised rapidly.

Anthocyans, however, can recycle a spent glutathione molecule (2).

For this reason the combination of the reduced glutathione or n-

acetyl cysteine with anthocyans is particularly powerful, increasing

the antioxidant properties of both several fold. According to

research by Professor Gernot Treusch and colleagues in Germany this

combination is more powerful than any known antioxidant.

The anthocyanidins, sometimes abbreviated to anthocyans, provide more

dramatic colours such as those found in black grapes, bilberries and

cranberries and tend to have a more powerful antioxidant effect.

Included in this group are many substances now concentrated in food

supplements for their antioxidant effect. These include:

Quercitin (from cranberry)

Rutin (from buckwheat)

Astragalin (from astragulus mushrooms)

Hesperidin (from citrus)

Catechin (from grapes)

Pycnogenol (from pine bark)

Silymarin (from milk thistle)

Bilberry

Ginkgo biloba

2. Ohlenschlager G & Truesch G. Reduced glutathione and anthocyans -

redox cycling and redox recycling in biological systems (unpublished -

copy held in Lamberts Library at ION)

Still trying to find the link of a combo that fixes the BBB (blood

brain barrier), but I don't know that they have shown it is

compromised in CFS

Zymitol: compare to Wobenzym: zymitol contains alot of stuff

http://www.911healthshop.com/zysyenctovio.html

Protease is another form of " proteolytic " enzymes which demonstrates

the ability to breakdown protein. Its role is critical as incomplete

protein digestion creates problems for the body including development

of allergies and formation of toxic substances. Undigested proteins

have been linked to many health concerns and foreign invaders of the

intestinal tract.*

Some of these foreign invaders are parasites. Protease is largely

responsible for keeping the small intestines free from parasites. A

lack of protease increases the risk of intestinal infection including

an overgrowth of the yeast Candida.* (I don't think this is an issue

as these proteases are all over

the intestinal villi)...

Curcurmin and glutathione S-transferase: http://www.ift.org/cms/?

pid=1000992

Curry could enhance enzyme's antioxidant potential: curcurmin

increases GST

Curcumin, a phenolic agent and the yellow pigment in curry, has been

found to enhance the antioxidant properties of hemeoxygenase (HO-1),

an enzyme critical to fighting oxidation in the brain. Oxidative

damage by free radicals is believed to factor in age-related

neurodegenerative disorders such as Alzheimer's disease. A team of

U.S. and Italian researchers studied the effects of various levels of

curcumin on rat astrocytes and rat hippocampal nuerons. Results

showed that treatment of astrocytes with curcumin strongly increased

expression of the HO-1 protein at both the cytoplasmatic and nuclear

level. Two other detoxification enzymes, quinone reductase and

gluthathione S-transferase, were also enhanced when treated with

curcumin. Hippocampal neurons pre-incubated with curcumin showed a

stronger cellular resistance to oxidative agents. The research will

be presented this week at the American Physiological Society's annual

conference in Washington, D.C.

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?

cmd=Retrieve & db=PubMed & list_uids=9744529 & dopt=Abstract

Even though the levels of various hepatic GST isoenzymes were

significantly increased upon curcumin feeding, maximum induction was

noticed for the pi class isoenzyme (mGSTP1-1)

Pet with CFS?

http://www.animallovers.com/products/dogs/ImmunoRejuv.htm

but that reminded me about the TMG that I shelved awhile back

SAMe too expensive?

http://lifeextensionvitamins.com/lifeextensionvitamins/tmg1.html

Nice overall antioxidant article showing interactions of C, ALA, GSH

www.vetmedpub.com/cp/pdf/special_reports/hills_8.pdf

http://www.loudzen.com/canary/metabolic_basis/detoxification/p450.html

(Good stuff on MCS and links)

the P450 drug interactions link from the above site: link now dead

so...

medicine.iupui.edu/flockhart/table.htm (dead also)

or google p450 drug interactions

www.uchsc.edu/sm/psych/ppfr/cyp_metabolism.htm

www.atforum.com/SiteRoot/pages/ addiction_resources/P450%20Drug%

20Interactions.PDF

just a thought but if you have multiple drugs affecting the same

detox enzyme this could be trouble...

note that grapefruit juice can increase levels of some drugs

and still trying to change the bond angle of my water