Re: PCR usefulness

[Guest guest]

Hi a

I have microscope images that show there are long thin filaments in my

blood. These have been there since I started looking 6 months ago. My

healthy partner has none or very few (bit of uncertainty here). As I

said earlier, I have no orthodox diagnosis but I do have my suspicions.

Pictures here:

http://ivytree.users.btopenworld.com/

Sue

> Sue,

> If you have treated an infection with samento or an antibiotic chances

are

> there won't be much of the bacteria in your blood. Thus a PCR may come

up

> negative when you are still infected. Lots of people think that the

PCR is

> the most accurate. Well, it is when the infection is still in the

blood

> stream. A positive test is REALLY positive. But a negative test may

just

> mean it wasn't in THAT sample of blood. Not sure what you are testing

for,

> but if it is borrelia you could get the urine antigen test while on an

> antibiotic for a week. This is from Igenex, if you are interested.

Nothing

> is perfect.

> a Carnes

[Guest guest]

Re: PCR usefulness

> Hi a

>

> I have microscope images that show there are long thin filaments in my

> blood. These have been there since I started looking 6 months ago. My

> healthy partner has none or very few (bit of uncertainty here). As I

> said earlier, I have no orthodox diagnosis but I do have my suspicions.

> Pictures here:

> http://ivytree.users.btopenworld.com/

>

> Sue

Sue... re: slide 14 (plus Samento) - did you do a slide containing only

Samento to compare for present plant matter?

Kezzi. . .

[Guest guest]

No, good point, I didn't.

Sue

>

> Sue... re: slide 14 (plus Samento) - did you do a slide containing

only

> Samento to compare for present plant matter?

>

> Kezzi. . .

[Guest guest]

> I have microscope images that show there are long thin filaments in my blood.

> These have been there since I started looking 6 months ago. My healthy

> partner has none or very few (bit of uncertainty here). As I said earlier, I

> have no orthodox diagnosis but I do have my suspicions. Pictures here:

> http://ivytree.users.btopenworld.com/

Hi,

This is absolutely fascinating! Can you tell us more about what you are

doing and why?

Unfortunately, I was unable to open your graphs and photos on your website.

Sue B.,

Upstate New York

[Guest guest]

> > I have microscope images that show there are long thin filaments

in my blood.

>> Hi,

>

> This is absolutely fascinating! Can you tell us more about what

you are

> doing and why?

>

> Unfortunately, I was unable to open your graphs and photos on your

website.

>

> Sue B.,

> Upstate New York

Hi Sue,

I could be that those filaments in the blood is Fibrin build-up or

the Amyloid formation that was mentioned by Alan Cocchetto in his CFS

article.

Al

[Guest guest]

Hi Sue

> This is absolutely fascinating! Can you tell us more about what you

are

> doing and why?

>

> Unfortunately, I was unable to open your graphs and photos on your

website.

A few people have said they're having problems while others seem to be

ok. I think there must be a problem with the server, I know that I was

having difficulty seeing the images yesterday. But I can assure you

they are there!

[Guest guest]

Hi Sue cont.

(Finger slipped & it flew off before I'd finished!)

I have a diagnosis of ME (CFS). I've been told I might have

borreliosis but I haven't had any Lyme tests. When I was

told about the possibility of borrelia we (partner & me) decided to have

a look at my blood to see what was there. The pictures are of filaments

& clusters of filaments that we see in my blood and not in my partner's.

Quite simply, I don't know what they are and I have no way of telling.

It seems to me that the only thing I can do is check them regularly and

see if I can find anything that affects them. I'm quite sure I don't

want them - I was absolutely horrified the first time I saw a cluster of

filament-type things wriggling in *my* blood.

I've been checking for the past 6 months. They haven't gone away

but the numbers did seem to decrease around December/January before

increasing again.

Sue

[Guest guest]

Sue

1) Do you feel any response to the rife machine when you subject

yourself to it?

2) Is there any reason to believe that spirochetes could survive for

80 hours on a slide outside the human body (and possibly multiply) ?

(I really don't know--but have heard spirochetes are difficult to

culture).

3) Is your microscope actually powerful enough to see a spirochete?

4) Do your symptoms and the number of filaments correspond?

Thanks

Kell

> Hi Sue cont.

> (Finger slipped & it flew off before I'd finished!)

>

> I have a diagnosis of ME (CFS). I've been told I might have

> borreliosis but I haven't had any Lyme tests. When I was

> told about the possibility of borrelia we (partner & me) decided to

have

> a look at my blood to see what was there. The pictures are of

filaments

> & clusters of filaments that we see in my blood and not in my

partner's.

> Quite simply, I don't know what they are and I have no way of

telling.

> It seems to me that the only thing I can do is check them regularly

and

> see if I can find anything that affects them. I'm quite sure I don't

> want them - I was absolutely horrified the first time I saw a

cluster of

> filament-type things wriggling in *my* blood.

>

> I've been checking for the past 6 months. They haven't gone away

> but the numbers did seem to decrease around December/January before

> increasing again.

> Sue

[Guest guest]

Kell,

To reply to your questions:

> 1) Do you feel any response to the rife machine when you subject

> yourself to it?

I haven't rifed myself yet. The coil is low current about 1A, which I

believe is much lower than most rife machines (10A+?) so it may not have

any effect on me at all.

> 2) Is there any reason to believe that spirochetes could survive for

> 80 hours on a slide outside the human body (and possibly multiply) ?

> (I really don't know--but have heard spirochetes are difficult to

> culture).

It's an interesting point that spirochetes are difficult to culture.

Firstly, I don't know that they're spirochetes. Secondly, if they are,

they may be emerging from cysts rather than multiplying. The blood is

sealed between the slide and a coverslip so it dries very slowly. I've

seen two-week-old slides where there was still Brownian motion in the

debris, so there was still fluid present. Without the data to back it

up, I would say that the number of filaments decreases after a while. I

shall have to put checking that on my ToDo list!

> 3) Is your microscope actually powerful enough to see a spirochete?

I'm not a biologist so I come at this knowing very little about what I

should & shouldn't see in blood. I'm a physicist & my partner is an

electronics engineer.

There are things that puzzle me. A borrelia spirochete has a width of

~0.5um according to the literature. This is around the wavelength of

light, so we should be right at the limit of resolution if we're seeing

borrelia. The filaments that I'm seeing don't spiral like the

literature suggests spirochetes should. I can't see any internal detail

but then I wouldn't expect to. But its a good microscope.

> 4) Do your symptoms and the number of filaments correspond?

Very roughly, probably. I can't be any more definite than that because

formal counting only started in the past month or so, when I've been

feeling pretty lousy. I was also feeling pretty poor in October when

there were many. I felt brighter in December & early January when I

think I saw fewer. I wish I could be more positive.

Reading through, what I've said is all very equivocal and that's what I

feel about it - I don't have enough data and/or knowledge to take a

position on this.

Sue

>

> Thanks

>

> Kell