Misclassification of recent HIV-1 seroconversion in sub-Saharan Africa

[Guest guest]

Misclassification of recent HIV-1 seroconversion in sub-Saharan Africa using the

sensitive/less sensitive technique

In resource-limited settings where HIV-1 is endemic, there is a need for simple,

inexpensive but effective rapid methods for detecting recent infections and

estimating incidence for the purposes of surveillance and management. We sort to

determine possible reasons for reported misclassifications of recent HIV-1

seroconversion as determined with the S/LS assay in sub-Saharan

Africa.FindingsWe used the modified Determine HIV-1/2 sensitive/less sensitive

method for determining recent HIV-1 seroconversion to determine recent

infections among ELISA repeat HIV-1 positive samples from blood donors.

Furthermore, HIV-1 seropositivity was confirmed using a line immunoassay and the

results used to validate the performance of the modified Determine HIV-1/2 S/LS

assay. The results confirmed reported misclassifications of recent HIV-1

seroconversion in sub-Saharan Africa.

It was noted that, lack of confirmation of HIV-1 seropositivity in suspected

cases of HIV-1 contributed to misclassifications.

Conclusions: It was concluded that, with confirmation of HIV-1 seropositivity,

the modified Determine HIV-1/2 S/LS assay will be a rapid and cost effective

method for determining HIV-1 recent infections and estimating incidencein

resource-limited settings. The need for detailed studies to validate simple

methods for determining recent HIV-1 infections is emphasized.

Author: Kwabena Duedu HayfordKwamena Sagoe

Credits/Source: Virology Journal 2011, 8:176

http://7thspace.com/headlines/379424/misclassification_of_recent_hiv_1_seroconve\

rsion_in_sub_saharan_africa_using_the_sensitiveless_sensitive_technique.html

[Guest guest]

Misclassification of recent HIV-1 seroconversion in sub-Saharan Africa using the

sensitive/less sensitive technique

In resource-limited settings where HIV-1 is endemic, there is a need for simple,

inexpensive but effective rapid methods for detecting recent infections and

estimating incidence for the purposes of surveillance and management. We sort to

determine possible reasons for reported misclassifications of recent HIV-1

seroconversion as determined with the S/LS assay in sub-Saharan

Africa.FindingsWe used the modified Determine HIV-1/2 sensitive/less sensitive

method for determining recent HIV-1 seroconversion to determine recent

infections among ELISA repeat HIV-1 positive samples from blood donors.

Furthermore, HIV-1 seropositivity was confirmed using a line immunoassay and the

results used to validate the performance of the modified Determine HIV-1/2 S/LS

assay. The results confirmed reported misclassifications of recent HIV-1

seroconversion in sub-Saharan Africa.

It was noted that, lack of confirmation of HIV-1 seropositivity in suspected

cases of HIV-1 contributed to misclassifications.

Conclusions: It was concluded that, with confirmation of HIV-1 seropositivity,

the modified Determine HIV-1/2 S/LS assay will be a rapid and cost effective

method for determining HIV-1 recent infections and estimating incidencein

resource-limited settings. The need for detailed studies to validate simple

methods for determining recent HIV-1 infections is emphasized.

Author: Kwabena Duedu HayfordKwamena Sagoe

Credits/Source: Virology Journal 2011, 8:176

http://7thspace.com/headlines/379424/misclassification_of_recent_hiv_1_seroconve\

rsion_in_sub_saharan_africa_using_the_sensitiveless_sensitive_technique.html