Vitamin D + polyps

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Tacalcitol is a synthetic derivative of vitamin D. Interesting...

the link:

http://www.ncbi.nlm.nih.gov/pubmed/18551997

[The change of proliferative potential of fibroblasts derived of nasal polyps in

vitro cultured, influenced by derivatives vitamins D]

[Article in Polish]

Rostkowska-Nadolska B, Ku & #347;mierz D, Kapral M, Latocha M, Swiatkowska L,

Fraczek M.

Katedra i Klinika Otolaryngologii Akademii Medycznej we Wroc & #322;awiu.

beatanm@...

INTRODUCTION: Recurrent polyposis in the same patient resulting in the necessity

of repeated surgeries forced to search for new pharmacological therapeutic

methods. At present, locally acting glycocorticosteroids have the greatest value

in the treatment of nasal polyposis. Polyps grow is connected with inflammation

process and proliferation of fibroblasts. OBJECTIVE: An evaluation of calcitriol

and tacalcitol influence on proliferation of fibroblasts extracted from nasal

polyps. MATERIAL: consisted of 9 tissue samples coming from nasal polyps sampled

during polypectomies. The testing was performed on the polyps fibroblasts after

the sixth passage after the primary culture was established. Three days after

the culture was started the cells were poured with nutrient medium without serum

added and after further 24 hours was replaced by nutrient medium with takalcitol

and calcitriol in the defined concentrations. The expression of the genes coding

histone H3 was evaluated with the use of RT-PCR technique. RESULTS: Tacalcitiol

and calcitriol in vitro decrease proliferation of fibroblasts sampled from nasal

polyps. Inhibition is most effective for the concentration of 10-4M. Tacalcitiol

and calcitriol also inhibit level of histone H3 gene expression. CONCLUSION:

Experimental data suggest tacalcitiol to be more effective in the same

concentration. Present studies may indicate the direction of further

investigation in the potential pharmacological treatment on nasal polyps.