Re: Grape seed extract vs polyps

[Guest guest]

Great article, thank you! I rushed out yesterday to buy some Grape Seed Extract

but accidentally came home with GrapeFRUIT Seed Extract and suffered a pretty

bad reaction. I am assuming there is a big difference between the two?

>

> A while ago, I believe we discussed the potential interest of using VEGF

> inhibitors against polyps, notably in the view of articles such as the

> following one :

>

> Am J Respir Crit Care Med. <http://www.ncbi.nlm.nih.gov/pubmed/19762561>

> 2009 Dec 1;180(11):1056-67. Epub 2009 Sep 17.

> Vascular endothelial growth factor drives autocrine epithelial cell

> proliferation and survival in chronic rhinosinusitis with nasal

> polyposis.

> Lee HS

> <http://www.ncbi.nlm.nih.gov/pubmed?term=%22Lee%20HS%22%5BAuthor%5D> ,

> Myers A

> <http://www.ncbi.nlm.nih.gov/pubmed?term=%22Myers%20A%22%5BAuthor%5D> ,

> Kim J

> <http://www.ncbi.nlm.nih.gov/pubmed?term=%22Kim%20J%22%5BAuthor%5D> .

>

> s Hopkins Asthma and Allergy Center, 5501 Hopkins Bayview Circle,

> Room 3B65A, Baltimore, MD 21224, USA.

> Abstract

> RATIONALE: The pathogenesis of nasal polyps in chronic rhinosinusitis is

> poorly understood.

>

> OBJECTIVES: These studies seek to implicate a functional role for

> vascular endothelial growth factor (VEGF) in perpetuating primary nasal

> epithelial cell overgrowth, a key feature of hyperplastic polyps.

>

> METHODS: Comparison of VEGF and receptor expression was assessed by

> ELISA of nasal lavage, immunohistochemistry of sinus tissue, flow

> cytometry of nasal epithelial cells, and ELISA of supernatants.

> VEGF-dependent cell growth and apoptosis were assessed with blocking

> antibodies to VEGF, their receptors, or small interfering RNA knockdown

> of neuropilin-1 by cell proliferation assays and flow cytometric binding

> of annexin V.

>

> MEASUREMENTS AND MAIN RESULTS: VEGF protein was sevenfold higher in

> nasal lavage from patients with polyposis compared with control subjects

> (P < 0.001). We also report elevated expression of VEGF (P < 0.012),

> receptors VEGFR2 and phospho-VEGFR2 (both P < 0.04), and identification

> of VEGF coreceptor neuropilin-1 in these tissues. Nasal epithelial cells

> from patients with polyps demonstrated faster growth rates (P < 0.005).

> Exposure of cells to blocking antibodies against VEGF resulted in

> inhibition of cell growth (P < 0.05). VEGF receptor blockade required

> blockade of neuropilin-1 (P < 0.05) and resulted in increased apoptosis

> (P < 0.001) and inhibition of autocrine epithelial VEGF production (P <

> 0.05).

>

> CONCLUSIONS: These data demonstrate that VEGF is a novel biomarker for

> chronic rhinosinusitis with hyperplastic sinonasal polyposis that

> functions in an autocrine feed-forward manner to promote nasal

> epithelial cell growth and to inhibit apoptosis. These findings

> implicate a previously unrecognized and novel role of VEGF functioning

> through neuropilin-1 on nonneoplastic primary human airway epithelial

> cells, to amplify cell growth, contributing to exuberant hyperplastic

> polyposis.

>

> And we identified, among others, grape seed extract as a potential VEGF

> inhibitor :

>

> Cancer Prev Res (Phila Pa).

> <http://www.ncbi.nlm.nih.gov/pubmed/19139005> 2008 Dec;1(7):554-61.

> Grape seed extract inhibits angiogenesis via suppression of the vascular

> endothelial growth factor receptor signaling pathway.

> Wen W

> <http://www.ncbi.nlm.nih.gov/pubmed?term=%22Wen%20W%22%5BAuthor%5D> , Lu

> J <http://www.ncbi.nlm.nih.gov/pubmed?term=%22Lu%20J%22%5BAuthor%5D> ,

> Zhang K

> <http://www.ncbi.nlm.nih.gov/pubmed?term=%22Zhang%20K%22%5BAuthor%5D> ,

> Chen S

> <http://www.ncbi.nlm.nih.gov/pubmed?term=%22Chen%20S%22%5BAuthor%5D> .

>

> Department of Molecular Medicine, Beckman Research Institute of the City

> of Hope, 1500 East Duarte Road, Duarte, CA 91010, USA. wwen@...

> Abstract

> Blockade of angiogenesis is an important approach for cancer treatment

> and prevention. Vascular endothelial growth factor (VEGF) is one of the

> most critical factors that induce angiogenesis and has thus become an

> attractive target for antiangiogenesis treatment. However, most current

> anti-VEGF agents often cause some side effects when given chronically.

> Identification of naturally occurring VEGF inhibitors derived from diet

> would be one alternative approach with an advantage of known safety.

> Grape seed extract (GSE), a widely used dietary supplement, is known to

> have antitumor activity. In this study, we have explored the activity of

> GSE on VEGF receptor and angiogenesis. We found that GSE could directly

> inhibit the kinase activity of purified VEGF receptor 2, a novel

> activity of GSE that has not been characterized. GSE could also inhibit

> the VEGF receptor/mitogen-activated protein kinase-mediated signaling

> pathway in endothelial cells. As a result, GSE could inhibit

> VEGF-induced endothelial cell proliferation and migration as well as

> sprout formation from aorta ring. In vivo assay further showed that GSE

> could inhibit tumor growth and tumor angiogenesis of MDA-MB-231 breast

> cancer cells in mice. Consistent with the in vitro data, GSE treatment

> of tumor-bearing mice led to concomitant reduction of blood vessel

> density and phosphorylation of mitogen-activated protein kinase.

> Depletion of polyphenol with polyvinylpyrrolidone abolished the

> antiangiogenic activity of GSE, suggesting a water-soluble fraction of

> polyphenol in GSE is responsible for the antiangiogenic activity. Taken

> together, this study indicates that GSE is a well-tolerated and

> inexpensive natural VEGF inhibitor and could potentially be useful in

> cancer prevention or treatment.

>

>

> Well, a fellow sinusitis sufferer has reported interesting results on

> alt.sinusitis :

> Here's a brief history of my sinus problem: I've had nose polyps

> since around year 2000. I had surgery in 2003 to remove them and been

> using

> Nasonex (Aqueous Mometasone Furoate Monohydrate) to keep them

> from growing back. But they keep (or have grown) back.

>

> I now use NeilMed Sinus Rinse to help clear mucus. But it still

> wasn't enough as things became unbearable two weeks ago. So I recently

> asked a doctor if there are natural things I could take to keep

> polyps from growing or shrink them or at least lower the production of

> mucus.

>

>

> Beginning two weeks ago, I started taking pill per day of

> 50 mg Grape Seed Extract which it says contains Vitis Vinifera seed,

> 95% polyphenols and 80% proanthocyanidins. And after a few days,

> one nostril became unclogged and I can now breath through it.

> But I don't know if it has shrunk a few polyps or simply minimized

> mucus. All I know is I can now breath through one nostril.

>

>

> The pharmacist also suggested I try, Butter Bur or Thymus Extract or

> Tinospora Cordifolia or EPA. Does anyone know if these are better

> than the grape seed extract?

>

>

> Are there any natural remedies things to take to shrink them?

>

>

>

>

> Given that oral Grape Seed Extract yields interesting results, I would

> be willing to bet that topical GSE could be quite efficient against

> nasal polyps because of superior doses delivered locally.

>

> There is however a potential risk in the case of Samter's, because GSE

> is a COX-2 inhibitor (see abstract below). There is no mention of GSE a

> COX-1 inhibitor like aspirin, nor is there any mention of it being a

> leukotriene inhibitor. This means that there may be a negative reaction

> to GSE in the most sensitive of us (ie, those who are sensitive to COX-2

> inhibitors), but the rest, as well as all those who are desensed, can

> probably apply it topically in a dilute and short-term fashion without

> much risk. At any rate, a negative reaction would mean that one is

> sensitive to it and should stop.

>

>

>

>

>

> Pharm Res. <http://www.ncbi.nlm.nih.gov/pubmed/20143255> 2010

> Jun;27(6):1092-102. Epub 2010 Feb 9.

> Dietary grape seed proanthocyanidins inhibit UVB-induced

> cyclooxygenase-2 expression and other inflammatory mediators in

> UVB-exposed skin and skin tumors of SKH-1 hairless mice.

> Sharma SD

> <http://www.ncbi.nlm.nih.gov/pubmed?term=%22Sharma%20SD%22%5BAuthor%5D>

> , Katiyar SK

> <http://www.ncbi.nlm.nih.gov/pubmed?term=%22Katiyar%20SK%22%5BAuthor%5D>

> .

>

> Department of Dermatology, University of Alabama, 1670 University

> Boulevard, Volker Hall 557, Birmingham, Alabama 35294, USA.

> Abstract

> PURPOSE: The purpose of this study was to determine the chemopreventive

> mechanism of dietary grape seed proanthocyanidins (GSPs) against

> ultraviolet (UV) radiation-induced skin tumor development in mice.

>

> METHODS: Six-to-seven-week-old SKH-1 hairless mice were subjected to

> photocarcinogenesis protocol, and exposed to UVB radiation (180

> mJ/cm(2)) three times/week for 24 weeks. Mice were fed a standard AIN76A

> control diet with or without supplementation with grape seed

> proanthocyanidins (GSPs; 0.2% or 0.5%, w/w). At the termination of the

> experiment, mice were sacrificed, and skin and skin tumor samples were

> harvested and subjected to the analysis of biomarkers related to

> inflammation using immunostaining, western blot analysis, ELISA and

> real-time PCR.

>

> RESULTS: Dietary GSPs inhibited UVB-induced infiltration of

> proinflammatory leukocytes and the levels of myeloperoxidase,

> cyclooxygenase-2 (COX-2), prostaglandin (PG) E(2), cyclin D1 and

> proliferating cell nuclear antigen (PCNA) in the skin and skin tumors

> compared to non-GSPs-treated UVB irradiated mouse skin and skin tumors.

> GSPs also significantly inhibited the levels of proinflammatory

> cytokines, tumor necrosis factor-alpha (P < 0.01), IL-1beta (P < 0.001)

> and IL-6 (P < 0.001), in UVB-exposed skin and skin tumors.

>

> CONCLUSION: The results from this study clearly suggest that dietary

> GSPs inhibit photocarcinogenesis in mice through the inhibition of

> UVB-induced inflammation and mediators of inflammation in mouse skin.

>

[Guest guest]

Sorry to hear about the bad reaction to Grapefruit Seed Extract. Please let

us know if you have a similar problem with Grape Seed Extract. I bought some

GSE a couple of months ago and have been hesitant to use it.

Re: Grape seed extract vs polyps

Great article, thank you! I rushed out yesterday to buy some Grape Seed

Extract but accidentally came home with GrapeFRUIT Seed Extract and suffered

a pretty bad reaction. I am assuming there is a big difference between the

two?

> There is however a potential risk in the case of Samter's, because GSE

> is a COX-2 inhibitor (see abstract below). There is no mention of GSE a

> COX-1 inhibitor like aspirin, nor is there any mention of it being a

> leukotriene inhibitor. This means that there may be a negative reaction

> to GSE in the most sensitive of us (ie, those who are sensitive to COX-2

> inhibitors), but the rest, as well as all those who are desensed, can

> probably apply it topically in a dilute and short-term fashion without

> much risk. At any rate, a negative reaction would mean that one is

> sensitive to it and should stop.

>

>

[Guest guest]

Jules,They are altogether very different compounds. Grapefruit seed extract is considered an antibacterial, so it is possible it burned you if you attempted to irrigate with it. I should have added a warning that both are often confusingly abbreviated as GSE - very sorry about that. At any rate, if you want to irrigate with grape seed extract, start with a very dilute solution to see what the effects are, and then increase very progressively the dosage if nothing happens. Alternatively, you can eat it if it is under pill form, as suggested by the testimony.> >> > A while ago, I believe we discussed the potential interest of using VEGF> > inhibitors against polyps, notably in the view of articles such as the> > following one :> > > > Am J Respir Crit Care Med. <http://www.ncbi.nlm.nih.gov/pubmed/19762561>> > 2009 Dec 1;180(11):1056-67. Epub 2009 Sep 17.> > Vascular endothelial growth factor drives autocrine epithelial cell> > proliferation and survival in chronic rhinosinusitis with nasal> > polyposis.> > Lee HS> > <http://www.ncbi.nlm.nih.gov/pubmed?term=%22Lee%20HS%22%5BAuthor%5D> ,> > Myers A> > <http://www.ncbi.nlm.nih.gov/pubmed?term=%22Myers%20A%22%5BAuthor%5D> ,> > Kim J> > <http://www.ncbi.nlm.nih.gov/pubmed?term=%22Kim%20J%22%5BAuthor%5D> .> > > > s Hopkins Asthma and Allergy Center, 5501 Hopkins Bayview Circle,> > Room 3B65A, Baltimore, MD 21224, USA.> > Abstract> > RATIONALE: The pathogenesis of nasal polyps in chronic rhinosinusitis is> > poorly understood.> > > > OBJECTIVES: These studies seek to implicate a functional role for> > vascular endothelial growth factor (VEGF) in perpetuating primary nasal> > epithelial cell overgrowth, a key feature of hyperplastic polyps.> > > > METHODS: Comparison of VEGF and receptor expression was assessed by> > ELISA of nasal lavage, immunohistochemistry of sinus tissue, flow> > cytometry of nasal epithelial cells, and ELISA of supernatants.> > VEGF-dependent cell growth and apoptosis were assessed with blocking> > antibodies to VEGF, their receptors, or small interfering RNA knockdown> > of neuropilin-1 by cell proliferation assays and flow cytometric binding> > of annexin V.> > > > MEASUREMENTS AND MAIN RESULTS: VEGF protein was sevenfold higher in> > nasal lavage from patients with polyposis compared with control subjects> > (P < 0.001). We also report elevated expression of VEGF (P < 0.012),> > receptors VEGFR2 and phospho-VEGFR2 (both P < 0.04), and identification> > of VEGF coreceptor neuropilin-1 in these tissues. Nasal epithelial cells> > from patients with polyps demonstrated faster growth rates (P < 0.005).> > Exposure of cells to blocking antibodies against VEGF resulted in> > inhibition of cell growth (P < 0.05). VEGF receptor blockade required> > blockade of neuropilin-1 (P < 0.05) and resulted in increased apoptosis> > (P < 0.001) and inhibition of autocrine epithelial VEGF production (P <> > 0.05).> > > > CONCLUSIONS: These data demonstrate that VEGF is a novel biomarker for> > chronic rhinosinusitis with hyperplastic sinonasal polyposis that> > functions in an autocrine feed-forward manner to promote nasal> > epithelial cell growth and to inhibit apoptosis. These findings> > implicate a previously unrecognized and novel role of VEGF functioning> > through neuropilin-1 on nonneoplastic primary human airway epithelial> > cells, to amplify cell growth, contributing to exuberant hyperplastic> > polyposis.> > > > And we identified, among others, grape seed extract as a potential VEGF> > inhibitor :> > > > Cancer Prev Res (Phila Pa).> > <http://www.ncbi.nlm.nih.gov/pubmed/19139005> 2008 Dec;1(7):554-61.> > Grape seed extract inhibits angiogenesis via suppression of the vascular> > endothelial growth factor receptor signaling pathway.> > Wen W> > <http://www.ncbi.nlm.nih.gov/pubmed?term=%22Wen%20W%22%5BAuthor%5D> , Lu> > J <http://www.ncbi.nlm.nih.gov/pubmed?term=%22Lu%20J%22%5BAuthor%5D> ,> > Zhang K> > <http://www.ncbi.nlm.nih.gov/pubmed?term=%22Zhang%20K%22%5BAuthor%5D> ,> > Chen S> > <http://www.ncbi.nlm.nih.gov/pubmed?term=%22Chen%20S%22%5BAuthor%5D> .> > > > Department of Molecular Medicine, Beckman Research Institute of the City> > of Hope, 1500 East Duarte Road, Duarte, CA 91010, USA. wwen@> > Abstract> > Blockade of angiogenesis is an important approach for cancer treatment> > and prevention. Vascular endothelial growth factor (VEGF) is one of the> > most critical factors that induce angiogenesis and has thus become an> > attractive target for antiangiogenesis treatment. However, most current> > anti-VEGF agents often cause some side effects when given chronically.> > Identification of naturally occurring VEGF inhibitors derived from diet> > would be one alternative approach with an advantage of known safety.> > Grape seed extract (GSE), a widely used dietary supplement, is known to> > have antitumor activity. In this study, we have explored the activity of> > GSE on VEGF receptor and angiogenesis. We found that GSE could directly> > inhibit the kinase activity of purified VEGF receptor 2, a novel> > activity of GSE that has not been characterized. GSE could also inhibit> > the VEGF receptor/mitogen-activated protein kinase-mediated signaling> > pathway in endothelial cells. As a result, GSE could inhibit> > VEGF-induced endothelial cell proliferation and migration as well as> > sprout formation from aorta ring. In vivo assay further showed that GSE> > could inhibit tumor growth and tumor angiogenesis of MDA-MB-231 breast> > cancer cells in mice. Consistent with the in vitro data, GSE treatment> > of tumor-bearing mice led to concomitant reduction of blood vessel> > density and phosphorylation of mitogen-activated protein kinase.> > Depletion of polyphenol with polyvinylpyrrolidone abolished the> > antiangiogenic activity of GSE, suggesting a water-soluble fraction of> > polyphenol in GSE is responsible for the antiangiogenic activity. Taken> > together, this study indicates that GSE is a well-tolerated and> > inexpensive natural VEGF inhibitor and could potentially be useful in> > cancer prevention or treatment.> > > > > > Well, a fellow sinusitis sufferer has reported interesting results on> > alt.sinusitis :> > Here's a brief history of my sinus problem: I've had nose polyps> > since around year 2000. I had surgery in 2003 to remove them and been> > using> > Nasonex (Aqueous Mometasone Furoate Monohydrate) to keep them> > from growing back. But they keep (or have grown) back.> > > > I now use NeilMed Sinus Rinse to help clear mucus. But it still> > wasn't enough as things became unbearable two weeks ago. So I recently> > asked a doctor if there are natural things I could take to keep> > polyps from growing or shrink them or at least lower the production of> > mucus.> > > > > > Beginning two weeks ago, I started taking pill per day of> > 50 mg Grape Seed Extract which it says contains Vitis Vinifera seed,> > 95% polyphenols and 80% proanthocyanidins. And after a few days,> > one nostril became unclogged and I can now breath through it.> > But I don't know if it has shrunk a few polyps or simply minimized> > mucus. All I know is I can now breath through one nostril.> > > > > > The pharmacist also suggested I try, Butter Bur or Thymus Extract or> > Tinospora Cordifolia or EPA. Does anyone know if these are better> > than the grape seed extract?> > > > > > Are there any natural remedies things to take to shrink them?> > > > > > > > > > Given that oral Grape Seed Extract yields interesting results, I would> > be willing to bet that topical GSE could be quite efficient against> > nasal polyps because of superior doses delivered locally.> > > > There is however a potential risk in the case of Samter's, because GSE> > is a COX-2 inhibitor (see abstract below). There is no mention of GSE a> > COX-1 inhibitor like aspirin, nor is there any mention of it being a> > leukotriene inhibitor. This means that there may be a negative reaction> > to GSE in the most sensitive of us (ie, those who are sensitive to COX-2> > inhibitors), but the rest, as well as all those who are desensed, can> > probably apply it topically in a dilute and short-term fashion without> > much risk. At any rate, a negative reaction would mean that one is> > sensitive to it and should stop.> > > > > > > > > > > > Pharm Res. <http://www.ncbi.nlm.nih.gov/pubmed/20143255> 2010> > Jun;27(6):1092-102. Epub 2010 Feb 9.> > Dietary grape seed proanthocyanidins inhibit UVB-induced> > cyclooxygenase-2 expression and other inflammatory mediators in> > UVB-exposed skin and skin tumors of SKH-1 hairless mice.> > Sharma SD> > <http://www.ncbi.nlm.nih.gov/pubmed?term=%22Sharma%20SD%22%5BAuthor%5D>> > , Katiyar SK> > <http://www.ncbi.nlm.nih.gov/pubmed?term=%22Katiyar%20SK%22%5BAuthor%5D>> > .> > > > Department of Dermatology, University of Alabama, 1670 University> > Boulevard, Volker Hall 557, Birmingham, Alabama 35294, USA.> > Abstract> > PURPOSE: The purpose of this study was to determine the chemopreventive> > mechanism of dietary grape seed proanthocyanidins (GSPs) against> > ultraviolet (UV) radiation-induced skin tumor development in mice.> > > > METHODS: Six-to-seven-week-old SKH-1 hairless mice were subjected to> > photocarcinogenesis protocol, and exposed to UVB radiation (180> > mJ/cm(2)) three times/week for 24 weeks. Mice were fed a standard AIN76A> > control diet with or without supplementation with grape seed> > proanthocyanidins (GSPs; 0.2% or 0.5%, w/w). At the termination of the> > experiment, mice were sacrificed, and skin and skin tumor samples were> > harvested and subjected to the analysis of biomarkers related to> > inflammation using immunostaining, western blot analysis, ELISA and> > real-time PCR.> > > > RESULTS: Dietary GSPs inhibited UVB-induced infiltration of> > proinflammatory leukocytes and the levels of myeloperoxidase,> > cyclooxygenase-2 (COX-2), prostaglandin (PG) E(2), cyclin D1 and> > proliferating cell nuclear antigen (PCNA) in the skin and skin tumors> > compared to non-GSPs-treated UVB irradiated mouse skin and skin tumors.> > GSPs also significantly inhibited the levels of proinflammatory> > cytokines, tumor necrosis factor-alpha (P < 0.01), IL-1beta (P < 0.001)> > and IL-6 (P < 0.001), in UVB-exposed skin and skin tumors.> > > > CONCLUSION: The results from this study clearly suggest that dietary> > GSPs inhibit photocarcinogenesis in mice through the inhibition of> > UVB-induced inflammation and mediators of inflammation in mouse skin.> >>

[Guest guest]

Has anyone else had success with using GSE to control their polyps?

If so, what dose are you using?

Kent

[Guest guest]

Has anyone else had success with this? (oral grape seed extract tablets)ThanksFrom: asfy <asfyso@...>Subject: Grape seed extract vs polypssamters Date: Tuesday, September 7, 2010, 4:16 AM

A while ago, I believe we discussed the potential interest of using VEGF inhibitors against polyps, notably in the view of articles such as the following one :Am J Respir Crit Care Med. 2009 Dec 1;180(11):1056-67. Epub 2009 Sep 17.Vascular endothelial growth factor drives autocrine epithelial cell proliferation and survival in chronic rhinosinusitis with nasal polyposis.Lee HS, Myers A, Kim J.s Hopkins Asthma and Allergy Center, 5501 Hopkins Bayview Circle, Room 3B65A, Baltimore, MD 21224, USA.AbstractRATIONALE: The pathogenesis of nasal polyps in chronic rhinosinusitis is poorly understood.OBJECTIVES: These studies seek to implicate a functional role for vascular endothelial growth factor (VEGF) in perpetuating primary nasal epithelial cell overgrowth, a key feature of hyperplastic polyps.METHODS: Comparison of VEGF and receptor expression was assessed by ELISA of nasal lavage, immunohistochemistry of sinus tissue, flow cytometry of nasal epithelial cells, and ELISA of supernatants. VEGF-dependent cell growth and apoptosis were assessed with blocking antibodies to VEGF, their

receptors, or small interfering RNA knockdown of neuropilin-1 by cell proliferation assays and flow cytometric binding of annexin V.MEASUREMENTS AND MAIN RESULTS: VEGF protein was sevenfold higher in nasal lavage from patients with polyposis compared with control subjects (P < 0.001). We also report elevated expression of VEGF (P < 0.012), receptors VEGFR2 and phospho-VEGFR2 (both P < 0.04), and identification of VEGF coreceptor neuropilin-1 in these tissues. Nasal epithelial cells from patients with polyps demonstrated faster growth rates (P < 0.005). Exposure of cells to blocking antibodies against VEGF resulted in inhibition of cell growth (P < 0.05). VEGF receptor blockade required blockade of neuropilin-1 (P < 0.05) and resulted in increased apoptosis (P < 0.001) and inhibition of autocrine epithelial VEGF

production (P < 0.05).CONCLUSIONS: These data demonstrate that VEGF is a novel biomarker for chronic rhinosinusitis with hyperplastic sinonasal polyposis that functions in an autocrine feed-forward manner to promote nasal epithelial cell growth and to inhibit apoptosis. These findings implicate a previously unrecognized and novel role of VEGF functioning through neuropilin-1 on nonneoplastic primary human airway epithelial cells, to amplify cell growth, contributing to exuberant hyperplastic polyposis.And we identified, among others, grape seed extract as a potential VEGF inhibitor :Cancer Prev Res (Phila Pa). 2008 Dec;1(7):554-61.Grape seed extract inhibits angiogenesis via suppression of the vascular endothelial growth factor receptor signaling pathway.Wen W, Lu J, Zhang K, Chen S.Department of Molecular Medicine, Beckman Research Institute of the City of Hope, 1500 East Duarte Road, Duarte, CA 91010, USA. wwen@...AbstractBlockade of angiogenesis is an important approach for cancer treatment and prevention. Vascular endothelial growth factor (VEGF) is one of the most

critical factors that induce angiogenesis and has thus become an attractive target for antiangiogenesis treatment. However, most current anti-VEGF agents often cause some side effects when given chronically. Identification of naturally occurring VEGF inhibitors derived from diet would be one alternative approach with an advantage of known safety. Grape seed extract (GSE), a widely used dietary supplement, is known to have antitumor activity. In this study, we have explored the activity of GSE on VEGF receptor and angiogenesis. We found that GSE could directly inhibit the kinase activity of purified VEGF receptor 2, a novel activity of GSE that has not been characterized. GSE could also inhibit the VEGF receptor/mitogen-activated protein kinase-mediated signaling pathway in endothelial cells. As a result, GSE could inhibit VEGF-induced endothelial cell proliferation and migration as well as sprout formation from aorta ring. In vivo assay further showed

that GSE could inhibit tumor growth and tumor angiogenesis of MDA-MB-231 breast cancer cells in mice. Consistent with the in vitro data, GSE treatment of tumor-bearing mice led to concomitant reduction of blood vessel density and phosphorylation of mitogen-activated protein kinase. Depletion of polyphenol with polyvinylpyrrolidone abolished the antiangiogenic activity of GSE, suggesting a water-soluble fraction of polyphenol in GSE is responsible for the antiangiogenic activity. Taken together, this study indicates that GSE is a well-tolerated and inexpensive natural VEGF inhibitor and could potentially be useful in cancer prevention or treatment.Well, a fellow sinusitis sufferer has reported interesting results on alt.sinusitis :Here's a brief history of my sinus problem: I've had nose polyps since around year 2000. I had surgery in 2003 to remove them and been using Nasonex (Aqueous Mometasone Furoate Monohydrate) to keep them from growing back. But they keep (or have grown) back. I now use NeilMed Sinus Rinse to help clear mucus. But it still wasn't enough as things became

unbearable two weeks ago. So I recently asked a doctor if there are natural things I could take to keep polyps from growing or shrink them or at least lower the production of mucus. Beginning two weeks ago, I started taking pill per day of 50 mg Grape Seed Extract which it says contains Vitis Vinifera seed, 95% polyphenols and 80% proanthocyanidins. And after a few days, one nostril became unclogged and I can now breath through it. But I don't know if it has shrunk a few polyps or simply minimized mucus. All I know is I can now breath through one nostril. The pharmacist also suggested I try, Butter Bur or Thymus Extract or Tinospora Cordifolia or EPA. Does anyone know if these are better than the grape seed extract? Are there any natural remedies things to take to shrink

them? Given that oral Grape Seed Extract yields interesting results, I would be willing to bet that topical GSE could be quite efficient against nasal polyps because of superior doses delivered locally.There is however a potential risk in the case of Samter's, because GSE is a COX-2 inhibitor (see abstract below). There is no mention of GSE a COX-1 inhibitor like aspirin, nor is there any mention of it being a leukotriene inhibitor. This means that there may be a negative reaction to GSE in the most sensitive of us

(ie, those who are sensitive to COX-2 inhibitors), but the rest, as well as all those who are desensed, can probably apply it topically in a dilute and short-term fashion without much risk. At any rate, a negative reaction would mean that one is sensitive to it and should stop.Pharm Res. 2010 Jun;27(6):1092-102. Epub 2010 Feb 9.Dietary grape seed proanthocyanidins inhibit UVB-induced cyclooxygenase-2 expression and other inflammatory mediators in UVB-exposed skin and skin tumors of SKH-1 hairless mice.Sharma SD, Katiyar SK.Department of Dermatology, University of Alabama, 1670 University Boulevard,

Volker Hall 557, Birmingham, Alabama 35294, USA.AbstractPURPOSE: The purpose of this study was to determine the chemopreventive mechanism of dietary grape seed proanthocyanidins (GSPs) against ultraviolet (UV) radiation-induced skin tumor development in mice.METHODS: Six-to-seven-week-old SKH-1 hairless mice were subjected to photocarcinogenesis protocol, and exposed to UVB radiation (180 mJ/cm(2)) three times/week for 24 weeks. Mice were fed a standard AIN76A control diet with or without supplementation with grape seed proanthocyanidins (GSPs; 0.2% or

0.5%, w/w). At the termination of the experiment, mice were sacrificed, and skin and skin tumor samples were harvested and subjected to the analysis of biomarkers related to inflammation using immunostaining, western blot analysis, ELISA and real-time PCR.RESULTS: Dietary GSPs inhibited UVB-induced infiltration of proinflammatory leukocytes and the levels of myeloperoxidase, cyclooxygenase-2 (COX-2), prostaglandin (PG) E(2), cyclin D1 and proliferating cell nuclear antigen (PCNA) in the skin and skin tumors compared to non-GSPs-treated UVB irradiated mouse skin and skin tumors. GSPs also significantly inhibited the levels of proinflammatory cytokines, tumor necrosis factor-alpha (P < 0.01), IL-1beta (P < 0.001) and IL-6 (P < 0.001), in UVB-exposed skin and skin tumors.CONCLUSION: The results from this study clearly suggest that dietary GSPs inhibit photocarcinogenesis in mice through the inhibition of UVB-induced inflammation and mediators of inflammation in mouse skin.

[Guest guest]

My dr. just recomended grape seed extract along with some other supplements. Not sure the mechanism of action.

From: samters [mailto:samters ] On Behalf Of Sent: Sunday, November 14, 2010 11:29 AMsamters Subject: Re: Grape seed extract vs polyps

Has anyone else had success with this? (oral grape seed extract tablets)Thanks

From: asfy <asfyso@...>Subject: Grape seed extract vs polypssamters Date: Tuesday, September 7, 2010, 4:16 AM

A while ago, I believe we discussed the potential interest of using VEGF inhibitors against polyps, notably in the view of articles such as the following one :

Am J Respir Crit Care Med. 2009 Dec 1;180(11):1056-67. Epub 2009 Sep 17.

Vascular endothelial growth factor drives autocrine epithelial cell proliferation and survival in chronic rhinosinusitis with nasal polyposis.

Lee HS, Myers A, Kim J.

s Hopkins Asthma and Allergy Center, 5501 Hopkins Bayview Circle, Room 3B65A, Baltimore, MD 21224, USA.

Abstract

RATIONALE: The pathogenesis of nasal polyps in chronic rhinosinusitis is poorly understood.

OBJECTIVES: These studies seek to implicate a functional role for vascular endothelial growth factor (VEGF) in perpetuating primary nasal epithelial cell overgrowth, a key feature of hyperplastic polyps.

METHODS: Comparison of VEGF and receptor expression was assessed by ELISA of nasal lavage, immunohistochemistry of sinus tissue, flow cytometry of nasal epithelial cells, and ELISA of supernatants. VEGF-dependent cell growth and apoptosis were assessed with blocking antibodies to VEGF, their receptors, or small interfering RNA knockdown of neuropilin-1 by cell proliferation assays and flow cytometric binding of annexin V.

MEASUREMENTS AND MAIN RESULTS: VEGF protein was sevenfold higher in nasal lavage from patients with polyposis compared with control subjects (P < 0.001). We also report elevated expression of VEGF (P < 0.012), receptors VEGFR2 and phospho-VEGFR2 (both P < 0.04), and identification of VEGF coreceptor neuropilin-1 in these tissues. Nasal epithelial cells from patients with polyps demonstrated faster growth rates (P < 0.005). Exposure of cells to blocking antibodies against VEGF resulted in inhibition of cell growth (P < 0.05). VEGF receptor blockade required blockade of neuropilin-1 (P < 0.05) and resulted in increased apoptosis (P < 0.001) and inhibition of autocrine epithelial VEGF production (P < 0.05).

CONCLUSIONS: These data demonstrate that VEGF is a novel biomarker for chronic rhinosinusitis with hyperplastic sinonasal polyposis that functions in an autocrine feed-forward manner to promote nasal epithelial cell growth and to inhibit apoptosis. These findings implicate a previously unrecognized and novel role of VEGF functioning through neuropilin-1 on nonneoplastic primary human airway epithelial cells, to amplify cell growth, contributing to exuberant hyperplastic polyposis.

And we identified, among others, grape seed extract as a potential VEGF inhibitor :

Cancer Prev Res (Phila Pa). 2008 Dec;1(7):554-61.

Grape seed extract inhibits angiogenesis via suppression of the vascular endothelial growth factor receptor signaling pathway.

Wen W, Lu J, Zhang K, Chen S.

Department of Molecular Medicine, Beckman Research Institute of the City of Hope, 1500 East Duarte Road, Duarte, CA 91010, USA. wwen@...

Abstract

Blockade of angiogenesis is an important approach for cancer treatment and prevention. Vascular endothelial growth factor (VEGF) is one of the most critical factors that induce angiogenesis and has thus become an attractive target for antiangiogenesis treatment. However, most current anti-VEGF agents often cause some side effects when given chronically. Identification of naturally occurring VEGF inhibitors derived from diet would be one alternative approach with an advantage of known safety. Grape seed extract (GSE), a widely used dietary supplement, is known to have antitumor activity. In this study, we have explored the activity of GSE on VEGF receptor and angiogenesis. We found that GSE could directly inhibit the kinase activity of purified VEGF receptor 2, a novel activity of GSE that has not been characterized. GSE could also inhibit the VEGF receptor/mitogen-activated protein kinase-mediated signaling pathway in endothelial cells. As a result, GSE could inhibit VEGF-induced endothelial cell proliferation and migration as well as sprout formation from aorta ring. In vivo assay further showed that GSE could inhibit tumor growth and tumor angiogenesis of MDA-MB-231 breast cancer cells in mice. Consistent with the in vitro data, GSE treatment of tumor-bearing mice led to concomitant reduction of blood vessel density and phosphorylation of mitogen-activated protein kinase. Depletion of polyphenol with polyvinylpyrrolidone abolished the antiangiogenic activity of GSE, suggesting a water-soluble fraction of polyphenol in GSE is responsible for the antiangiogenic activity. Taken together, this study indicates that GSE is a well-tolerated and inexpensive natural VEGF inhibitor and could potentially be useful in cancer prevention or treatment.

Well, a fellow sinusitis sufferer has reported interesting results on alt.sinusitis :

Here's a brief history of my sinus problem: I've had nose polyps since around year 2000. I had surgery in 2003 to remove them and been using Nasonex (Aqueous Mometasone Furoate Monohydrate) to keep them from growing back. But they keep (or have grown) back.

I now use NeilMed Sinus Rinse to help clear mucus. But it still wasn't enough as things became unbearable two weeks ago. So I recently asked a doctor if there are natural things I could take to keep polyps from growing or shrink them or at least lower the production of mucus.

Beginning two weeks ago, I started taking pill per day of 50 mg Grape Seed Extract which it says contains Vitis Vinifera seed, 95% polyphenols and 80% proanthocyanidins. And after a few days, one nostril became unclogged and I can now breath through it. But I don't know if it has shrunk a few polyps or simply minimized mucus. All I know is I can now breath through one nostril.

The pharmacist also suggested I try, Butter Bur or Thymus Extract or Tinospora Cordifolia or EPA. Does anyone know if these are better than the grape seed extract?

Are there any natural remedies things to take to shrink them?

Given that oral Grape Seed Extract yields interesting results, I would be willing to bet that topical GSE could be quite efficient against nasal polyps because of superior doses delivered locally.

There is however a potential risk in the case of Samter's, because GSE is a COX-2 inhibitor (see abstract below). There is no mention of GSE a COX-1 inhibitor like aspirin, nor is there any mention of it being a leukotriene inhibitor. This means that there may be a negative reaction to GSE in the most sensitive of us (ie, those who are sensitive to COX-2 inhibitors), but the rest, as well as all those who are desensed, can probably apply it topically in a dilute and short-term fashion without much risk. At any rate, a negative reaction would mean that one is sensitive to it and should stop.

Pharm Res. 2010 Jun;27(6):1092-102. Epub 2010 Feb 9.

Dietary grape seed proanthocyanidins inhibit UVB-induced cyclooxygenase-2 expression and other inflammatory mediators in UVB-exposed skin and skin tumors of SKH-1 hairless mice.

Sharma SD, Katiyar SK.

Department of Dermatology, University of Alabama, 1670 University Boulevard, Volker Hall 557, Birmingham, Alabama 35294, USA.

Abstract

PURPOSE: The purpose of this study was to determine the chemopreventive mechanism of dietary grape seed proanthocyanidins (GSPs) against ultraviolet (UV) radiation-induced skin tumor development in mice.

METHODS: Six-to-seven-week-old SKH-1 hairless mice were subjected to photocarcinogenesis protocol, and exposed to UVB radiation (180 mJ/cm(2)) three times/week for 24 weeks. Mice were fed a standard AIN76A control diet with or without supplementation with grape seed proanthocyanidins (GSPs; 0.2% or 0.5%, w/w). At the termination of the experiment, mice were sacrificed, and skin and skin tumor samples were harvested and subjected to the analysis of biomarkers related to inflammation using immunostaining, western blot analysis, ELISA and real-time PCR.

RESULTS: Dietary GSPs inhibited UVB-induced infiltration of proinflammatory leukocytes and the levels of myeloperoxidase, cyclooxygenase-2 (COX-2), prostaglandin (PG) E(2), cyclin D1 and proliferating cell nuclear antigen (PCNA) in the skin and skin tumors compared to non-GSPs-treated UVB irradiated mouse skin and skin tumors. GSPs also significantly inhibited the levels of proinflammatory cytokines, tumor necrosis factor-alpha (P < 0.01), IL-1beta (P < 0.001) and IL-6 (P < 0.001), in UVB-exposed skin and skin tumors.

CONCLUSION: The results from this study clearly suggest that dietary GSPs inhibit photocarcinogenesis in mice through the inhibition of UVB-induced inflammation and mediators of inflammation in mouse skin.

[Guest guest]

I purchased a bottle of grape seed extract tablets some time ago and I've been hesitant to try them in fear of some negative reaction. Like you I've been waiting to see if some one has previous experience with the product.

Grape seed extract vs polypssamters Date: Tuesday, September 7, 2010, 4:16 AM

A while ago, I believe we discussed the potential interest of using VEGF inhibitors against polyps, notably in the view of articles such as the following one :

Am J Respir Crit Care Med. 2009 Dec 1;180(11):1056-67. Epub 2009 Sep 17.

Vascular endothelial growth factor drives autocrine epithelial cell proliferation and survival in chronic rhinosinusitis with nasal polyposis.

Lee HS, Myers A, Kim J.

s Hopkins Asthma and Allergy Center, 5501 Hopkins Bayview Circle, Room 3B65A, Baltimore, MD 21224, USA.

Abstract

RATIONALE: The pathogenesis of nasal polyps in chronic rhinosinusitis is poorly understood.

OBJECTIVES: These studies seek to implicate a functional role for vascular endothelial growth factor (VEGF) in perpetuating primary nasal epithelial cell overgrowth, a key feature of hyperplastic polyps.

METHODS: Comparison of VEGF and receptor expression was assessed by ELISA of nasal lavage, immunohistochemistry of sinus tissue, flow cytometry of nasal epithelial cells, and ELISA of supernatants. VEGF-dependent cell growth and apoptosis were assessed with blocking antibodies to VEGF, their receptors, or small interfering RNA knockdown of neuropilin-1 by cell proliferation assays and flow cytometric binding of annexin V.

MEASUREMENTS AND MAIN RESULTS: VEGF protein was sevenfold higher in nasal lavage from patients with polyposis compared with control subjects (P < 0.001). We also report elevated expression of VEGF (P < 0.012), receptors VEGFR2 and phospho-VEGFR2 (both P < 0.04), and identification of VEGF coreceptor neuropilin-1 in these tissues. Nasal epithelial cells from patients with polyps demonstrated faster growth rates (P < 0.005). Exposure of cells to blocking antibodies against VEGF resulted in inhibition of cell growth (P < 0.05). VEGF receptor blockade required blockade of neuropilin-1 (P < 0.05) and resulted in increased apoptosis (P < 0.001) and inhibition of autocrine epithelial VEGF production (P < 0.05).

CONCLUSIONS: These data demonstrate that VEGF is a novel biomarker for chronic rhinosinusitis with hyperplastic sinonasal polyposis that functions in an autocrine feed-forward manner to promote nasal epithelial cell growth and to inhibit apoptosis. These findings implicate a previously unrecognized and novel role of VEGF functioning through neuropilin-1 on nonneoplastic primary human airway epithelial cells, to amplify cell growth, contributing to exuberant hyperplastic polyposis.

[Guest guest]

I've started taking it for the past month or so with no ill effects. I can't say

whether it has improved things yet or not. I would say it is too early to tell.

One thing that I have been doing that seems to have improved things is to take

600mg of ibuprofen in the evenings. I already take 650mg of aspirin in the

mornings. It seems to have reduced a lot of the inflammation in my sinuses. On

my last two visits to the ENT for my quarterly checkup, he has noticed a big

difference.

R