Activation-induced cytidine deaminase mRNA levels in chronic lymphocytic leukemia

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BlankActivation-induced cytidine deaminase mRNA levels in chronic lymphocytic

leukemia.

VS Hancer, M Kose, R Diz-Kucukkaya, AS Yavuz, and M Aktan

Leuk Lymphoma, December 6, 2010; .

Department of Medical Biology and Genetics, Faculty of Medicine, Istanbul Bilim

University, Istanbul, Turkey.

The Rai and Binet staging systems, which are used as standard methods for

evaluating the prognosis of chronic lymphocytic leukemia (CLL), have some

restrictions in identifying patients with early-stage CLL who will progress

rapidly. To solve this defect, other prognostic parameters have become important

in recent years. Intracellular up-regulation of the AID gene in the leukemic

lymphocytes of patients with CLL may be an important parameter for predicting

the progression of CLL. In this study, AID mRNA expression levels were evaluated

in 50 patients with CLL and 50 healthy controls. AID mRNA expression was

significantly higher in patients than in controls. We then evaluated AID mRNA

levels according to the stages of CLL. Regarding AID mRNA levels, patients with

Rai stages 0, I, and II were compared with patients with stages III and IV,

whereas patients with Binet stage A were compared with patients with Binet

stages B and C. In patients with higher-risk Rai stages III and IV and Binet

stages B and C, activation-induced cytidine deaminase (AID) mRNA levels were

also significantly higher. Additionally, we found that the mRNA levels of

patients with AID in CLL were eight-fold higher than those in control patients,

suggesting that AID overexpression promotes chromosomal abnormalities and is

associated with CLL progression and survival. For this reason, and because of

the simplicity of quantitative real-time PCR analysis, AID might be a useful

clinical parameter after its importance is confirmed in larger and multivariate

studies.

PMID: 21133730