[Bone marrow immunophenotypes of 112 cases of lymphoid system malignant diseases.]

April 19, 2007

[bone marrow immunophenotypes of 112 cases of lymphoid system malignant

diseases.]

JY Ling, XF Sun, SL Yan, LR He, ZJ Zhen, and Y Xia

Ai Zheng, April 1, 2007; 26(4): 418-22.

State Key Laboratory of Oncology in South China, Guangzhou, Guangdong, 510060,

P. R. China. gzsunxf@....

BACKGROUND & OBJECTIVE: Diagnosis of lymphocytic leukemia and non-Hodgkin's

lymphoma (NHL) is based on bone marrow morphology. Immunophenotyping will make

diagnosis more precise through analyzing the origin and differentiation status

of tumor, which is necessary for treatment and prognosis prediction. This study

was to analyze the immunophenotypic characteristics of lymphocytic leukemia and

NHL with bone marrow involvement using flow cytometry (FCM). METHODS: Bone

marrow specimens from 112 patients with lymphocytic leukemia or NHL with bone

marrow involvement were detected by FCM using antibodies of T, B and myeloid

cell series. Using CD45/SSC gating strategy, the samples were analyzed with 5

parameters (FSC, SSC, McAb1-FITC, McAb2-PE, CD45-cytochrome). RESULTS: In 45

cases of precursor B lymphoblastic leukemia/lymphoma (B-ALL/LBL), the antigens

were mainly CD19, CD10, TdT, CD34, HLA-DR, and CD20. In 32 cases of precursor T

lymphoblastic leukemia/lymphoma (T-ALL/LBL), the antigens were mainly CD7, CD5,

cytoplasmic (Cy)CD3, TdT, CD34, surface CD3 (sCD3), and HLA-DR. Of the 77 cases

of precursor ALL/LBL, 28(36.4%) expressed myeloid-associated antigens, such as

CD13 and CD33û 9 (20.0%) cases of B-ALL/LBL coexpressed CD20 and CD34û 28(87.5%)

cases of T-ALL/LBL coexpressed cyCD3 and TdT. Among the 35 cases of mature

B-cell malignancies, 17 cases of chronic lymphocytic leukemia/small lymphocytic

lymphoma (CLL/SLL) mainly expressed CD19, CD20, CD5, HLA-DR, with coexpression

of CD19 and CD5û 4 cases of diffuse large B-cell lymphoma (DLBCL) mainly

expressed CD19, CD20, CD10, and HLA-DRû 3 cases of Burkittos lymphoma (BL)

mainly expressed CD19, CD10, CD20, and sIgMû 1 case of mantle cell lymphoma

(MCL) expressed CD5, CD19, CD20, and HLA-DR. Among the 10 mature T-cell

malignancies, 5 cases of unspecialied peripheral T-cell lymphoma (PTCL) mainly

expressed sCD3, CD5 and CD7, CD4 or CD8û 1 case of anaplastic large cell

lymphoma (ALCL) expressed sCD3 and HLA-DRû 4 cases of NK/T-cell malignancies

expressed CD56 and HLA-DR, CD4 or CD8 or CD7. Mature lymphoid system

malignancies didnot express early antigens, such as CD34 and TdT, but expressed

myeloid-associated antigens, especially CD13 and CD33. CONCLUSION:

Multiparameter FCM can not only provide data of cell lineage and differentiation

status but also detect phenotypic aberrancies, which is helpful for minimal

residual disease detecting.

PMID: 17430665

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