Where FISH and Cytogenetics Vary - 13q14

[Guest guest]

Cancer Genet Cytogenet. 2006 Apr;166(2):152-6.

Metaphase cells with normal G-bands have cryptic interstitial

deletions in 13q14 detectable by fluorescence in situ hybridization

in B-cell chronic lymphocytic leukemia.

Stockero KJ, Fink SR, Smoley SA, Paternoster SF, Shanafelt TD, Call

TG, Zent CS, Van Dyke DL, Kay NE, Dewald GW.

Cytogenetics Laboratory.

Interphase fluorescence in situ hybridization (FISH) studies with

D13S319 show that deletions of 13q14 are common in B-cell chronic

lymphocytic leukemia (B-CLL). In contrast, conventional cytogenetic

studies in B-CLL seldom reveal abnormalities of chromosome 13. We

hypothesized that chromosome 13 anomalies might not be detected

because they are caused by cryptic deletions rather than by the

absence of dividing B-CLL cells.

To investigate this possibility, we used FISH with D13S319 to study

metaphases from 12 patients known to have 13q- by interphase FISH.

These same patients had normal chromosomes by conventional

cytogenetic studies. As a result of this study, we report evidence

that B-CLL metaphases with 13q- are not detected because these

deletions are often cryptic and not visible by standard G-banding.

PMID: 16631472 [PubMed - in process]