[Detection of molecular cytogenetic abnormalities in 30 patients with chronic lymphocytic leukemia by fluorescence in situ hybridization.]

March 3, 2009

[Detection of molecular cytogenetic abnormalities in 30 patients with chronic

lymphocytic leukemia by fluorescence in situ hybridization.]

D Dai, XQ Zhang, XZ Zhang, AL Su, L Zhang, SB Cao, and YL Xu

Zhongguo Shi Yan Xue Ye Xue Za Zhi, February 1, 2009; 17(1): 31-5.

Department of Hematology, The First Affilated Hospital, Nanjing Medical

University, Nanjing 210006, Jiangsu Province, China.

This study was aimed to investigate the significance of interphase fluorescence

in situ hybridization (FISH) in detecting +12, del (13q14), p53 and atm gene

deletion in chronic lymphocytic leukemia (CLL). FISH and a panel of probes (CEP

12, LSI D13S319, LSI p53, LSI atm) were used to detect molecular cytogenetic

abnormalities in 30 patients with CLL. Cytogenetic aberrations and their

relation with some other prognostic factors (peripheral lymphocyte count, Binet

stage, LDH level, ZAP-70 and so on) were analyzed. The results indicated that

out of the 30 CLL patients, molecular cytogenetic aberrations were found in 19

(63.3%) cases and 7 (23.3%) patients showed more than two kinds of

abnormalities. The most frequent abnormality detected was del (13q14) (43.3%),

followed by trisomy of chromosome 12 (23.3%), del (atm) (13.3%) and del (p53)

(10.0%). There were no significant differences between molecular cytogenetic

aberrations and sex, age, Binet stage, peripheral lymphocyte count, or the serum

levels of lactate dehydrogenase (LDH), beta(2)-microglobulin (beta(2)-MG), or

ZAP-70. The incidence of atm gene deletion was higher in the group of CD38 high

expression than that in the group of low expression (p = 0.035). It is concluded

that FISH is a rapid and sensitive technique in analysing molecular cytogenetic

abnormalities, but its prognostic significance in CLL needs to further

investigate.

PMID: 19236742

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