[Analysis of immunophenotype, lymphocytic subsets and NK cells in patients with B cell chronic lymphoid leukemia.]

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[Analysis of immunophenotype, lymphocytic subsets and NK cells in patients with

B cell chronic lymphoid leukemia.]

L Zhang, L Zhu, HW Wang, and LH Yang

Zhongguo Shi Yan Xue Ye Xue Za Zhi, February 1, 2009; 17(1): 36-9.

Department of Hematology, The Second Clinical Hospital of Shanxi Medical

University, Taiyuan 030001, Shanxi Province, China.

The aim of this study was to evaluate the values of immunophenotype of leukemic

cells in bone marrow (BM) or peripheral blood (PB), lymphocytic subsets and NK

cells of PB in diagnosis, therapy and prognosis of patients with B cell chronic

lymphoid leukemia (B-CLL). The samples of PB and BM from 67 patients with B-CLL

were collected and detected by multi-parameter flow cytometry. The results

showed that the percentage of lymphocytes in PB of 67 patients with B-CLL

obviously increased, as compared with normal persons, while CD3, CD4, CD8 and NK

cells decreased significantely (p < 0.01), and no CD4/CD8 ratio had notable

change (p > 0.05). In their immunophenotype results, the positive expression

rate of CD19 was highest (91.04%), followed by CD5 (80.60%), CD20 (76.12),

cyCD79a (74.63%), CD38 (43.28%), CD11c (42.86%), CD7 (41.94%), ZAP-70 (39.29%),

CD25 (0.00%); co-expression rate of CD5 and CD19 was 72.73%; the expression of

ZAP-70 was correlated with CD38 (p < 0.05). In conclusion, detection of cell

immunophenotypes, lymphocytic subsets in PB and BM and NK cells in PB contribute

to diagnosis of patients with B-CLL.

PMID: 19236743

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