Antioxidant enzymes activities and plasma levels of oxidative stress markers in B-chronic lymphocytic leukemia patients.

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BlankAntioxidant enzymes activities and plasma levels of oxidative stress

markers in B-chronic lymphocytic leukemia patients.

I Zelen, P Djurdjevic, S Popovic, M Stojanovic, V Jakovljevic, S Radivojevic, D

Baskic, and N Arsenijevic

J BUON, April 1, 2010; 15(2): 330-6.

Department of Biochemistry, Faculty of Medicine, University of Kragujevac, S.

Markovica 69, 34 000 Kragujevac, Serbia. izelen@...

PURPOSE: Overproduction of reactive oxygen species (ROS) intermediates above the

functional capability of cellular antioxidants may result in instability of

important macromolecules and represents the molecular basis of many diseases

including inflammation processes, cardiovascular alterations, cancer etc. The

purpose of this study was to determine plasma level of superoxide anion,

hydrogen-peroxide and malondialdehyde (MDA) as markers of oxidative stress and

activities of superoxide dismutase (SOD), catalase (CAT) and glutathione

peroxidase (GPx) as antioxidant enzymes in B-chronic lymphocytic leukemia

(B-CLL) patients. METHODS: The study included 29 untreated B-CLL patients in

stage A, and 21 in stages B and C, classified according to the Binet system; 31

healthy volunteers formed the control group. After centrifugation of heparinized

peripheral blood, plasma levels of all investigated parameters were determined

using spectrophotometric methods. RESULTS: Plasma CAT activity was increased in

B-CLL patients compared with control subjects; also, progression of disease was

related with significantly higher plasma activity of CAT. Also, B-CLL patients

showed significantly higher plasma concentration of MDA compared with controls.

No statistically significant differences of superoxide anion and hydrogen

peroxide as well as plasma activity of SOD and GPx between the tested groups

were noted. CONCLUSION: Increase of CAT activity in B-CLL patients indicates

that there is stimulation of the antioxidant enzyme system, while the increase

of MDA concentration shows increased lipid peroxidation level. According to

these results it could be concluded that an imbalance exists between oxidants

and antioxidants in the plasma of B-CLL patients.

PMID: 20658731