[Immunoglobulin variable heavy chain region genetic constitution and mutation status in Chinese patients with chronic lymphocytic leukemia.]

April 14, 2009

[immunoglobulin variable heavy chain region genetic constitution and mutation

status in Chinese patients with chronic lymphocytic leukemia.]

Y Zhang, L Chen, W Xu, C Qiao, S Qian, H Qiu, K Miao, H Liu, and J Li

Zhonghua Yi Xue Yi Chuan Xue Za Zhi, April 1, 2009; 26(2): 196-9.

Department of Hematology, the Affiliated Hospital, Nantong University, Nantong,

Jiangsu, 226001 P. R. China. lijianyonglm@....

OBJECTIVE: To evaluate the genetic constitution and mutation status of the

immunoglobulin variable heavy chain region (IGVH) gene expression in Chinese

patients with chronic lymphocytic leukemia (CLL). METHODS: The IGVH mutation was

detected by multiplex PCR and direct sequencing of the purified PCR products

from 64 CLL patients. The segments of VH, DH and JH family and mutations were

analyzed by IMGT/V-QUEST and IGBlast. RESULTS: In the 64 patients, the most

common usage was VH3 (31/64, 48%), followed by VH4 (26/64, 41%), VH1 (4/64, 6%),

VH2 (2/64, 3%) and VH7 (1/64, 2%). The results also showed that 44 patients

(69%) had mutated VH, 6 cases (9%) had identical germline sequences. Among the

64 sequences of DH segments, DH3 gene family was used most frequently (25/64,

39%), among which 11 cases had unmutated VH. The most frequent usage of the JH

segments was JH6. CONCLUSION: There is significant difference in the frequency

of the IGVH gene family in Chinese CLL patients compared to Western patients,

suggesting the involvement of antigen selection in different ethnic and/or

environmental factors in CLL disease initiation, and its prognostic significance

needs further investigation.

PMID: 19350515

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