[OT] Researchers Detect Variations in DNA That Underlie Seven Common Diseases

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June 7, 2007

Researchers Detect Variations in DNA That Underlie Seven Common Diseases

By NICHOLAS WADE

Applying a new genomic technique to a large group of patients, researchers in

Britain have detected DNA variations that underlie seven common diseases,

discovering unexpected links between them.

The variations pinpoint biological pathways underlying each of the diseases, and

researchers hope that as the pathways are analyzed, new drugs and treatments

will emerge.

The seven common diseases are bipolar disorder, coronary artery disease, Crohn's

disease, hypertension, rheumatoid arthritis, and Type 1 and Type 2 diabetes.

Unveiling the complex genetics of common diseases was the promised payoff of the

$3 billion human genome project, completed in 2003, but progress was slow until

the recent development of devices that in a single operation can read the DNA

sequence at up to 500,000 points across an individual's genome. With the

devices, called chips, researchers can compare large numbers of patients with

healthy individuals, looking for points of differences in their genomes that may

be associated with disease.

The approach is known as whole genome association, and studies on Type 2

diabetes, heart disease and breast cancer have been reported within the last few

weeks. Those and the new study, which was financed by the Wellcome Trust of

London, demonstrate the power and reliability of the whole genome association

method, which stands in contrast to the many uncorroborated claims of disease

genes made previously.

" It's now absolutely clear that this is a new dawn in the genetics of common

human diseases, " said Donnelly, a statistical geneticist at Oxford

University who was chairman of the consortium of 50 institutions involved in the

Wellcome Trust study.

The consortium compared 2,000 patients with each disease from across Britain

with 3,000 healthy individuals as controls, half of whom were born in a single

week in 1958. The consortium's findings are published in today's Nature, along

with reports from two groups that largely confirm the consortium's genomic hits

in independent patient groups suffering from Crohn's disease and Type 1

diabetes.

The consortium discovered some 24 variants strongly linked to disease, about

half of which have been found already by other groups and half of which are new.

Among its most interesting findings is that genetic variants close to a gene

known as PTPN2 are associated with both Crohn's disease and Type 1 diabetes. The

link may be that both are autoimmune diseases and that the gene helps regulate

the immune system. Researchers hope that analysis of the gene's operations may

produce a treatment for the two diseases.

The consortium also found a genetic variant on chromosome 7 that carries a high

risk of rheumatoid arthritis for women, but none for men. Very few such variants

are known in diseases common to both sexes, Dr. Donnelly said.

Anne Bowcock, a geneticist at the Washington University School of Medicine in

St. Louis, said the Wellcome Trust study was a " tour de force " that established

how large-scale studies should be conducted.

Marie Nierras, an official of the Juvenile Diabetes Research Foundation, which

supported the companion study of Type 1 diabetes, said the research was " a

significant advance that identifies additional pathways that need to be looked

at. "

Dr. Kari Stefansson, chief executive of DeCode Genetics, an Icelandic company

that has dominated the search for common disease genes until the arrival of the

whole genome association method, said the Wellcome Trust study was " a large body

of work, done by very good people " but that it " hadn't come up with any big

discovery. "

Dr. Stefansson said the study had been delayed because of problems with the

Affymetrix chip it used, and would have had greater impact in the fast-moving

field if it had appeared several months earlier.

In the course of screening for any geographically related genetic bias, the

Wellcome Trust researchers discovered a southeast-to-northwest gradient across

Britain composed of 13 genes. The genes were probably under natural selection in

the people who became the island's first inhabitants.

One of the genes, which arose among Europe's first cattle herders 5,000 years

ago, enables people to drink milk in adulthood, an ability known as lactose

tolerance. The other genes, the researchers speculate, may confer resistance to

former scourges like pellagra, tuberculosis and leprosy.

Another possible source of statistical bias relates to race. The Wellcome Trust

consortium asked their 17,000 subjects to identify their race, then genetically

tested them and excluded 153 people who had non-European ancestry. The procedure

is necessary because whole genome association studies look for small differences

between patients and controls, which can be confounded by the genetic

differences between races.

The Wellcome Trust's findings apply to populations of European descent and need

to be verified in other races. Geneticists do not yet know what proportion of

the genetic variants associated with common disease will be found in all races,

but hope the overall biology will be much the same.

" The genetics gives us a whole new foothold into the biology of disease, " Dr.

Donnelly said. " If you find a variant in one population you might learn a lot

about that disease which is relevant to the disease in other populations. "

Because whole genome association studies work only in people of a single race,

researchers are concerned that follow-up studies should then be done in other

races. " I think everyone is committed to make sure that is the case, " said Mark

Daly, a geneticist at Massachusetts General Hospital.

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