Jump to content
RemedySpot.com

ZAP-70 Strong Adverse Prognostic Factor

Rate this topic


Guest guest

Recommended Posts

Original Article

Leukemia advance online publication 2 November 2006;

ZAP-70 expression, as detected by immunohistochemistry on bone

marrow biopsies from early-phase CLL patients, is a strong adverse

prognostic factor

R Zanotti1, A Ambrosetti1, M Lestani2, P Ghia3, C Pattaro4, A Remo2,

F Zanetti1, S Stella5, O Perbellini1, G Prato5, G Guida5, F

Caligaris-Cappio3,5, F Menestrina2, G Pizzolo1 and M Chilosi2

1Department of Clinical and Experimental Medicine, University of

Verona, Verona, Italy

2Department of Pathology, University of Verona, Verona, Italy

3Department of Oncology, Università Vita-Salute San Raffaele and

Istituto Scientifico San Raffaele, Milano, Italy

4Department of Medicine and Public Health, University of Verona,

Verona, Italy

5Institute for Cancer Research and Treatment, IRCC, Candiolo e

Ospedale Mauriziano 'Umberto I', Torino, Italy

Correspondence: Professor A Ambrosetti, Dipartimento di Medicina

Clinica e Sperimentale, Sezione di Ematologia, Università di Verona,

Policlinico G.B. Rossi., Piazzale L.A. Scuro 10, 37134 Verona,

Italy.

Received 20 April 2006; Revised 19 September 2006; Accepted 26

September 2006; Published online 2 November 2006.

Abstract

Zeta-associated protein-70 (ZAP-70), mostly assessed by flow-

cytometry (FC), recently emerged as reliable prognostic factor in

chronic lymphocytic leukaemia (CLL) at presentation.

We evaluated ZAP-70 expression in 156 CLL patients by

immunohistochemistry (IHC) on formalin-fixed bone marrow (BM)

biopsies at diagnosis. At presentation, 117 patients (75%) were with

Binet stage A, 27 (17%) stage B and 12 (8%) stage C. Median follow-

up was 61 months (range 6–242).

ZAP-70 was expressed in neoplastic lymphocytes of 69 patients (44%).

Concordance between ZAP-70 by IHC and ZAP-70 by FC, immunoglobulin

heavy chain variable genes (IGHV) mutational status and CD38

expression was found in 41/46 (89%), 41/49 (80%) and in 60/88 (68%)

tested cases, respectively.

ZAP-70 expression significantly correlated with advanced Binet stage

(B–C), diffuse BM infiltration, increased lactate dehydrogenase

(LDH) and 2-microglobulin serum levels and lymphocyte doubling time

<12 months.

ZAP-70 positivity was significantly related to poorer time to

progression (median 16 months vs 158 of ZAP-70-negative cases)

(P<0.0001) and overall survival (median 106 months vs not reached)

(P=0.0002); this correlation was confirmed at multivariate analysis.

ZAP-70 expression correlated with poorer outcome also when evaluated

only in the 117 stage A patients.

In conclusion, immunohistological detection of ZAP-70 on formalin-

fixed BM biopsies at diagnosis appears a useful methodological

approach to identify patients with poor prognosis in CLL.

Link to comment
Share on other sites

Join the conversation

You are posting as a guest. If you have an account, sign in now to post with your account.
Note: Your post will require moderator approval before it will be visible.

Guest
Reply to this topic...

×   Pasted as rich text.   Paste as plain text instead

  Only 75 emoji are allowed.

×   Your link has been automatically embedded.   Display as a link instead

×   Your previous content has been restored.   Clear editor

×   You cannot paste images directly. Upload or insert images from URL.

Loading...
×
×
  • Create New...