Determining Mutation Status Using PCR

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Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2006 Dec;14(6):1101-4.

[Detection of IgVH Mutation Status in Patients with Chronic

Lymphocytic Leukemia by Multiplex PCR.][Article in Chinese]

Zheng WJ, Chen LJ, Wu YJ, Li L, Xu W, Li JY.

Department of Hematology, The First Affiliated Hospital of Nanjing

Medical University, Jiangsu Province People Hospital, Nanjing

210029, China.

IgVH mutation status is one of the most important independent

prognostic factor of chronic lymphocytic leukemia (CLL).

In order to evaluate IgVH mutation status in patients with CLL, IgVH

mutation was detected by multiplex PCR in 9 CLL patients and

purified PCR amplification products were directly sequenced, IgH

somatic hypermutation and mutation site were analysed by IMGT/V-

QUEST.

The results showed that 5 patients had mutated IgVH, and IgVHs were

IGHV3-11*03, IGHV3-9*01, IGHV3-23*01, IGHV4-59*01, IGHV4-34*02,

respectively; whereas 4 others had unmutated IgVH, these IgVHs were

IGHV3-53*01, IGHV3-23*03, IGHV3-33*05 and IGHV3-7*01.

It is concluded that multiplex PCR is a rapid and easy method to

detect IgVH mutation status, and it solves the limitations and

pitfalls of routine PCR, and it is worth being extensively used both

in clinic and scientific researches.

PMID: 17204172 [PubMed - in process]