Guest guest Posted August 28, 2010 Report Share Posted August 28, 2010 The following study found 1 Hz to cause a peak of stem cell viability. Interestingly enough, 1 hertz is also the frequency at which electroporation/transfection is the most. see www.dragonfly75.com/eng/transfection.htmlAlternating current electric field effects on neural stem cell viability and differentiation.Matos MA, Cicerone MT.Polymers Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA. mmatos@...AbstractMethods utilizing stem cells hold tremendous promise for tissue engineering applications; however, many issues must be worked out before these therapies can be routinely applied. Utilization of external cues for preimplantation expansion and differentiation offers a potentially viable approach to the use of stem cells in tissue engineering. The studies reported here focus on the response of murine neural stem cells encapsulated in alginate hydrogel beads to alternating current electric fields. Cell viability and differentiation was studied as a function of electric field magnitude and frequency. We applied fields of frequency (0.1-10) Hz, and found a marked peak in neural stem cell viability under oscillatory electric fields with a frequency of 1 Hz. We also found an enhanced propensity for astrocyte differentiation over neuronal differentiation in the 1 Hz cultures, as compared to the other field frequencies we studied.http://docs.google.com/viewer?a=v & q=cache:Ycj3mu5E27UJ:www.bentham.org/crr/sample/CRR-5-1/D0006R.pdf+frequency+hz+%22stem+cell+%22+-528 & hl=en & gl=us & pid=bl & srcid=ADGEESifrR-tgZ-NBHkAZdYEXL9xnUP-7j6hAwiNINmbYuZCmv23qklHBcfJkFBKlPq1ndX9jmGu_p2bFYqv_fUjoe5AzVZ0HuMbyxpo5Qm7pTYEID0JufoGplEQ64_D3hhfKdu-PXrx & sig=AHIEtbQMM64uZuWPAkcY82JW0snk_U2gSA Quote Link to comment Share on other sites More sharing options...
Guest guest Posted August 30, 2010 Report Share Posted August 30, 2010 Interesting, I was just reading what you wrote from a Google search.My question is about 1 HZ ac generation - sine or square? It does make a difference. What is the cheapest or best Beck machine to produce this?Connect with foot pads, ear lobes or screen pads at selected locations? From: Forrest <a57ngel@...>Subject: 1 Hz Date: Saturday, August 28, 2010, 5:12 PM The following study found 1 Hz to cause a peak of stem cell viability. Interestingly enough, 1 hertz is also the frequency at which electroporation/transfection is the most. see www.dragonfly75.com/eng/transfection.htmlAlternating current electric field effects on neural stem cell viability and differentiation.Matos MA, Cicerone MT.Polymers Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA. mmatos@...AbstractMethods utilizing stem cells hold tremendous promise for tissue engineering applications; however, many issues must be worked out before these therapies can be routinely applied. Utilization of external cues for preimplantation expansion and differentiation offers a potentially viable approach to the use of stem cells in tissue engineering. The studies reported here focus on the response of murine neural stem cells encapsulated in alginate hydrogel beads to alternating current electric fields. Cell viability and differentiation was studied as a function of electric field magnitude and frequency. We applied fields of frequency (0.1-10) Hz, and found a marked peak in neural stem cell viability under oscillatory electric fields with a frequency of 1 Hz. We also found an enhanced propensity for astrocyte differentiation over neuronal differentiation in the 1 Hz cultures, as compared to the other field frequencies we studied.http://docs.google.com/viewer?a=v & q=cache:Ycj3mu5E27UJ:www.bentham.org/crr/sample/CRR-5-1/D0006R.pdf+frequency+hz+%22stem+cell+%22+-528 & hl=en & gl=us & pid=bl & srcid=ADGEESifrR-tgZ-NBHkAZdYEXL9xnUP-7j6hAwiNINmbYuZCmv23qklHBcfJkFBKlPq1ndX9jmGu_p2bFYqv_fUjoe5AzVZ0HuMbyxpo5Qm7pTYEID0JufoGplEQ64_D3hhfKdu-PXrx & sig=AHIEtbQMM64uZuWPAkcY82JW0snk_U2gSA Quote Link to comment Share on other sites More sharing options...
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