Guest guest Posted July 5, 2008 Report Share Posted July 5, 2008 I have printed all the file stuff but it's chinese to me for the most part and not sure which one staes what dr dan is saying if any of them Quote Link to comment Share on other sites More sharing options...
Guest guest Posted July 5, 2008 Report Share Posted July 5, 2008 what exactly are you looking for? a particular article or post? sorry, i got lost as to what you were trying to locate. > > I have printed all the file stuff but it's chinese to me for the most part and not sure which one staes what dr dan is saying if any of them > Quote Link to comment Share on other sites More sharing options...
Guest guest Posted July 5, 2008 Report Share Posted July 5, 2008 Jill print great study showing bacteria in bladder make EtG without drinking.All by themselves up to 70,000ng/mlNot only in bladder but Gut etc.Now all you need is a bacterium anywhere and and ethyl group from anywhereinstant false positive! This what Dr. Dan is saying...All the best,here if I can help...Clinical Chemistry 53: 1855-1857, 2007. First published August 23, 2007; 10.1373/clinchem.2007.089482 This Article Abstract Full Text (PDF) All Versions of this Article: clinchem.2007.089482v1 53/10/1855 most recent Submit an electronic Letter to the Editor about this paper Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via ISI Web of Science (1) Citing Articles via Google Scholar Google Scholar Articles by Helander, A. Articles by Dahl, H. Search for Related Content PubMed PubMed Citation Articles by Helander, A. Articles by Dahl, H. Related Collections General Clinical Chemistry Drug Monitoring and Toxicology Automation and Analytical Techniques (Clinical Chemistry. 2007;53:1855-1857.) © 2007 American Association for Clinical Chemistry, Inc. Technical Briefs Postcollection Synthesis of Ethyl Glucuronide by Bacteria in Urine May Cause False Identification of Alcohol Consumption Anders Helandera, Ingrid Olsson and Helen Dahl Department of Clinical Neuroscience, Karolinska Institute and Karolinska University Hospital, Stockholm, Sweden aaddress correspondence to this author at: Alcohol Laboratory, L7:03, Karolinska University Hospital Solna, SE-171 76 Stockholm, Sweden; fax 46-8-51771532, e-mail anders.helander@... Abstract Background: Ethyl glucuronide (EtG) is a minor ethanol metabolite used as a specific marker to document recent alcohol consumption; confirm abstinence in treatment programs, workplaces, and schools; and provide legal proof of drinking. This study examined if bacterial pathogens in urine may enable postsampling synthesis of EtG and ethyl sulfate (EtS) from ethanol, leading to clinical false-positive results. Methods: Urine specimens with confirmed growth of Escherichia coli, Klebsiella pneumoniae, or Enterobacter cloacae were stored at room temperature in the presence of ethanol. Ethanol was either added to the samples or generated by inoculation with the fermenting yeast species Candida albicans and glucose as substrate. EtG and EtS were measured by LC-MS. Results: High concentrations of EtG (24-h range 0.5–17.6 mg/L) were produced during storage in 35% of E. coli-infected urines containing ethanol. In some specimens that were initially EtG positive because of recent alcohol consumption, EtG was also sensitive to degradation by bacterial hydrolysis. In contrast, EtS was completely stable under these conditions. Conclusions: The presence of EtG in urine is not a unique indicator of recent drinking, but might originate from postcollection synthesis if specimens are infected with E. coli and contain ethanol. Given the associated risks for false identification of alcohol consumption and false-negative EtG results due to bacterial degradation, we recommend that measurement of EtG be combined with EtS, or in the future possibly replaced by EtS. Early recognition of problem drinking or relapse is important to ensure adequate alcohol treatment strategies (1). This goal has been hampered by a lack of sufficiently sensitive and specific diagnostic methods. The reliability of self-reporting is limited by denial and underreporting (2). The time frame for identifying alcohol use by ethanol testing is usually limited to <12 h, because of rapid metabolism and excretion (3). Research has therefore focused on developing alcohol biomarkers with a longer detection window (4). A new laboratory marker for detecting recent alcohol consumption is ethyl glucuronide (EtG) (5). EtG and ethyl sulfate (EtS) (6) are minor ethanol metabolites formed by uridine diphosphate–glucuronosyltransferase (UGT) and sulfotransferase (SULT), respectively, and excreted in urine for a longer time than ethanol (7)(8)(9)(10). Positive EtG and/or EtS test results thus provide a strong indication that the person has recently consumed alcohol, even when ethanol is no longer detectable (9). LC-MS methods are available for EtG and EtS detection (6)(10), as is an enzyme immunoassay for EtG (DRI® EtG, Microgenics). EtG has been recommended for forensic application (11)(12)(13) and is used for documentation of abstinence in treatment programs, for alcohol testing in the workplace and schools, and as legal proof of drinking (known as "the 80-h alcohol test"). However, the high diagnostic sensitivity of the EtG test has also produced adverse publicity (14), because unintentional ethanol intake from ethanol-based mouthwash (15) and hand sanitizers(16) may also generate positive results. The United States Substance Abuse and Mental Health Services Administration recently warned against using a positive EtG as primary or sole evidence of drinking for disciplinary and legal action (17). Bacterial contamination of urine may cause false-negative EtG test results (18). Many strains of Escherichia coli, the main source of urinary tract infections, contain the enzyme ß-glucuronidase, which hydrolyzes EtG. Given that UGT and SULT activity also occur with some bacteria (19)(20), we examined whether human pathogens may enable postcollection synthesis of EtG and EtS from ethanol in urine. From: JILL hall <jillypill@...>Subject: Re: etgEthylglucuronide Date: Saturday, July 5, 2008, 10:44 AM I have printed all the file stuff but it's chinese to me for the most part and not sure which one staes what dr dan is saying if any of them Quote Link to comment Share on other sites More sharing options...
Guest guest Posted July 5, 2008 Report Share Posted July 5, 2008 Bacterial and fungal infections are common in clinical practice, with E. coli being the primary pathogen responsible for urinary tract infections. Ethanol may be formed in unpreserved biological specimens because of microbial contamination and fermentation, and this risk is especially high in diabetic patients as a result of glycosuria. Accordingly, considering the potential serious disciplinary and legal consequences if an individual is falsely accused of alcohol consumption on the basis of an incorrect EtG result, caution is advised when interpreting EtG test results, and the risk for postcollection ethanol formation must be considered.(over 40% risk in samples tested)From: JILL hall <jillypill@...>Subject: Re: etgEthylglucuronide Date: Saturday, July 5, 2008, 10:44 AM I have printed all the file stuff but it's chinese to me for the most part and not sure which one staes what dr dan is saying if any of them Quote Link to comment Share on other sites More sharing options...
Guest guest Posted March 15, 2010 Report Share Posted March 15, 2010 Do all of the options at first lab test for etg? Quote Link to comment Share on other sites More sharing options...
Guest guest Posted March 16, 2010 Report Share Posted March 16, 2010 I pay $58 for option 1. It includes EtG and EtS. What state are you in?Sent from my iPhoneOn Mar 16, 2010, at 3:27, "carleton_lynch" <carleton_lynch@...> wrote: I'm paying $37.50 for option 1. I spoke with someone else in my group and he had option 1 every single time and it was abt that much (went up over 2 years). How do you know your is ETG? Did they tell you? We have 5 options and it goes from $31-81. Thanks > > > > What PRICE are you paying? Mine is $58 for Option 1 and it IS EtG. Ask others who are tested by your program. > > > etg > > Â > > > > > Do all of the options at first lab test for etg? > Quote Link to comment Share on other sites More sharing options...
Guest guest Posted March 16, 2010 Report Share Posted March 16, 2010 I was told in June when I got my 6th false positive etg to switch to first lab as they do both etg and ets. Sent from my iPhoneOn Mar 16, 2010, at 3:27, "carleton_lynch" <carleton_lynch@...> wrote: I'm paying $37.50 for option 1. I spoke with someone else in my group and he had option 1 every single time and it was abt that much (went up over 2 years). How do you know your is ETG? Did they tell you? We have 5 options and it goes from $31-81. Thanks > > > > What PRICE are you paying? Mine is $58 for Option 1 and it IS EtG. Ask others who are tested by your program. > > > etg > > Â > > > > > Do all of the options at first lab test for etg? > Quote Link to comment Share on other sites More sharing options...
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