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Re: Re: ETG raised????/redwood labs officially up to 1000ng/ml

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Why Redwood Toxicology Laboratory? Redwood Toxicology Laboratory (RTL) is one of the largest, full-service, independent drug and alcohol testing laboratories in the United States. RTL utilizes state of the art screening and confirmation methodologies for urine and oral fluid drug testing and is trusted by over 7,000 clients. RTL offers numerous advantages to your agency, including the following: Extensive experience in performing forensic toxicology analysis for alcohol and drugs of abuse, Highly qualified scientific staff, State of the art instrumentation for the detection of alcohol and drugs of abuse, Excellent client services, and; Extensive quality assurance and quality control procedures that help to ensure accurate results. What is Ethyl Glucuronide? Ethyl glucuronide (EtG) is a direct metabolite of beverage alcohol (ethanol). Its presence in urine may be used to detect recent alcohol consumption, even after ethanol is no longer measurable. The presence of EtG in urine is a definitive indicator that alcohol was ingested. How will my agency benefit by choosing RTL's EtG test? Key benefits of using RTL's EtG test include: Detects recent usage more accurately and for a longer period of time than standard testing Ideal for zero tolerance and abstinence situations Strong indicator of alcohol ingestion

within the previous 3 to 4 days EtG is only evident when alcohol is consumed and is not produced as a result of fermentation Allows monitoring in alcohol treatment programs Acts as an early warning system to detect trends towards relapse Tests are performed by LC/MS/MS on state of the art equipment for accuracy and reliability Thirty-six hour turnaround time from receipt of specimen EtG may be run on urine specimens in conjunction with other RTL drug testing panels Results available via internet, fax and U.S. mail Collection and shipping supplies provided at no additional cost Toll-free customer support and training services Expert witness and affidavit services available How long can EtG be detected in urine? Traditional laboratory methods detect the actual alcohol in the body, which reflects current use within the past few hours (depending on how much is

consumed). The presence of EtG in urine indicates that ethanol was ingested within the previous 3 to 4 days, or approximately 80 hours after ethanol is eliminated from the body. Therefore, EtG is a more accurate indicator of the recent ingestion of alcohol than measuring for the presence of ethanol itself. How accurate and reliable is the EtG test? EtG is a direct metabolite of alcohol (ethanol), and its detection in urine is highly specific, similar to testing for other drugs. Add to this, RTL utilizes the most sophisticated, sensitive, and specific equipment and technology available, LC/MS/MS, to screen, confirm, and quantitate EtG. This methodology provides highly accurate results. As is the case with any laboratory test, it is also very important to obtain clinical correlation. How much does RTL's EtG test cost? RTL's EtG test is $24.95 per specimen. Tested on state of the art equipment,

this affordable test allows for accurate and reliable results. How do I sign up for EtG testing? There are two easy ways to request EtG testing. Contact RTL. If you are not already a client, contact RTL to set up your account. Let us know that you are interested in EtG and indicate any other drugs you would like to test. Call us toll-free at (800) 255-2159. Choose "EtG" on your specimen labels. If you are an RTL client, simply choose "647 ETG" on your specimen collection / chain of custody labels. This code is pre-printed for all clients. Can residual EtG be detected in the urine of long-term alcoholics who abstain? Studies indicate that alcoholics in abstinence have no detectable levels of EtG in their urine after approximately 80 hours of detoxification. What about urine alcohol produced by fermentation? EtG is only detected in urine when alcohol is consumed. This is important since it is possible to have alcohol in urine without drinking. Alcohol in urine without drinking is due to the production of ethanol in vitro. Ethanol in vitro is spontaneously produced in the bladder or the specimen container itself, due to fermentation of urine samples containing sugars (diabetes) and yeast or bacteria. Since the ethanol produced is not metabolized by the liver, EtG will not be produced and will therefore not be detected in a urine containing alcohol as a result of fermentation. How stable is EtG in urine? Studies show that EtG is stable in urine for more than 4 days at room temperature. Recent experiments indicate that heating urine to 100?C actually increased the stability of EtG. Therefore, heat does not cause the breakdown of EtG, it actually increases stability. In addition, no artificial formation of EtG was found to occur

following the prolonged storage of urine at room temperature fortified with 1% ethanol. In general, what methods are used to detect EtG? What method does RTL use? Methods to detect EtG include immunoassay (EIA or ELISA), gas chromatography/mass spectrometry (GC/MS), liquid chromatography/mass spectrometry (LC/MS), and liquid chromatography/mass spectrometry/mass spectrometry (LC/MS/MS). Immunoassay methods are of limited specificity and sensitivity with a limit of detection of approximately 1000 ng/mL. GC/MS and LC/MS methods are much more specific than immunoassay, and offer detection limits of approximately 500 ng/mL. LC/MS/MS methods utilize the most technologically advanced instrumentation currently available in forensic and clinical toxicology and provide the highest sensitivity. Redwood utilizes an LC/MS/MS instrument for the confirmation of EtG, which has a limit of detection below


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