48-mer synthetic peptide analogue of the hepatitis B virus a determinant induces an anti-HBs antibody response after a single injection

[Guest guest]

1: J Med Virol 2000 Oct;62(2):159-66

48-mer synthetic peptide analogue of the hepatitis B virus " a " determinant

induces an anti-HBs antibody response after a single injection.

Moynihan JS, D'Mello FI, CR

Department of Pathology and Infectious Diseases, Royal Veterinary College,

London, United Kingdom.

[Medline record in process]

An extended (48 amino acid) synthetic peptide analogue of the hepatitus B

virus (HBV) S protein (HBsAg) 'a' determinant has been produced by using

9-fluorenylmethoxycarbonyl (fmoc) chemistry and a low substitution

polystyrene resin as the solid phase support. This peptide (S121/48)

elicited a sustained anti-peptide antibody response in BALB/c (H-2(d)) mice

when immunised with Freund's complete adjuvant (FCA). Cross-reactive,

anti-HBs antibodies were induced, directed against a significant proportion

of the conformationally restrained epitope repertoire on the native HBsAg

particles. Similar responses were obtained by injection of guinea pigs, a

species known both to be exquisitely sensitive to HBsAg and to produce a

wide range of B cell responses to HBsAg antigens. Taken together, these data

show for the first time, that a synthetic peptide mimicking conformational

epitopes can be produced by chemical synthesis and can be used to induce

significant titres of anti-HBs antibodies after a single injection. This

immunogen has considerable potential for incorporation into novel delivery

systems, e.g., microspheres, thus offering the potential of a controlled

release, single dose hepatitis B vaccine. Copyright 2000 Wiley-Liss, Inc.

PMID: 11002244, UI: 20459469

[Guest guest]

1: J Med Virol 2000 Oct;62(2):159-66

48-mer synthetic peptide analogue of the hepatitis B virus " a " determinant

induces an anti-HBs antibody response after a single injection.

Moynihan JS, D'Mello FI, CR

Department of Pathology and Infectious Diseases, Royal Veterinary College,

London, United Kingdom.

[Medline record in process]

An extended (48 amino acid) synthetic peptide analogue of the hepatitus B

virus (HBV) S protein (HBsAg) 'a' determinant has been produced by using

9-fluorenylmethoxycarbonyl (fmoc) chemistry and a low substitution

polystyrene resin as the solid phase support. This peptide (S121/48)

elicited a sustained anti-peptide antibody response in BALB/c (H-2(d)) mice

when immunised with Freund's complete adjuvant (FCA). Cross-reactive,

anti-HBs antibodies were induced, directed against a significant proportion

of the conformationally restrained epitope repertoire on the native HBsAg

particles. Similar responses were obtained by injection of guinea pigs, a

species known both to be exquisitely sensitive to HBsAg and to produce a

wide range of B cell responses to HBsAg antigens. Taken together, these data

show for the first time, that a synthetic peptide mimicking conformational

epitopes can be produced by chemical synthesis and can be used to induce

significant titres of anti-HBs antibodies after a single injection. This

immunogen has considerable potential for incorporation into novel delivery

systems, e.g., microspheres, thus offering the potential of a controlled

release, single dose hepatitis B vaccine. Copyright 2000 Wiley-Liss, Inc.

PMID: 11002244, UI: 20459469

[Guest guest]

1: J Med Virol 2000 Oct;62(2):159-66

48-mer synthetic peptide analogue of the hepatitis B virus " a " determinant

induces an anti-HBs antibody response after a single injection.

Moynihan JS, D'Mello FI, CR

Department of Pathology and Infectious Diseases, Royal Veterinary College,

London, United Kingdom.

[Medline record in process]

An extended (48 amino acid) synthetic peptide analogue of the hepatitus B

virus (HBV) S protein (HBsAg) 'a' determinant has been produced by using

9-fluorenylmethoxycarbonyl (fmoc) chemistry and a low substitution

polystyrene resin as the solid phase support. This peptide (S121/48)

elicited a sustained anti-peptide antibody response in BALB/c (H-2(d)) mice

when immunised with Freund's complete adjuvant (FCA). Cross-reactive,

anti-HBs antibodies were induced, directed against a significant proportion

of the conformationally restrained epitope repertoire on the native HBsAg

particles. Similar responses were obtained by injection of guinea pigs, a

species known both to be exquisitely sensitive to HBsAg and to produce a

wide range of B cell responses to HBsAg antigens. Taken together, these data

show for the first time, that a synthetic peptide mimicking conformational

epitopes can be produced by chemical synthesis and can be used to induce

significant titres of anti-HBs antibodies after a single injection. This

immunogen has considerable potential for incorporation into novel delivery

systems, e.g., microspheres, thus offering the potential of a controlled

release, single dose hepatitis B vaccine. Copyright 2000 Wiley-Liss, Inc.

PMID: 11002244, UI: 20459469

[Guest guest]

1: J Med Virol 2000 Oct;62(2):159-66

48-mer synthetic peptide analogue of the hepatitis B virus " a " determinant

induces an anti-HBs antibody response after a single injection.

Moynihan JS, D'Mello FI, CR

Department of Pathology and Infectious Diseases, Royal Veterinary College,

London, United Kingdom.

[Medline record in process]

An extended (48 amino acid) synthetic peptide analogue of the hepatitus B

virus (HBV) S protein (HBsAg) 'a' determinant has been produced by using

9-fluorenylmethoxycarbonyl (fmoc) chemistry and a low substitution

polystyrene resin as the solid phase support. This peptide (S121/48)

elicited a sustained anti-peptide antibody response in BALB/c (H-2(d)) mice

when immunised with Freund's complete adjuvant (FCA). Cross-reactive,

anti-HBs antibodies were induced, directed against a significant proportion

of the conformationally restrained epitope repertoire on the native HBsAg

particles. Similar responses were obtained by injection of guinea pigs, a

species known both to be exquisitely sensitive to HBsAg and to produce a

wide range of B cell responses to HBsAg antigens. Taken together, these data

show for the first time, that a synthetic peptide mimicking conformational

epitopes can be produced by chemical synthesis and can be used to induce

significant titres of anti-HBs antibodies after a single injection. This

immunogen has considerable potential for incorporation into novel delivery

systems, e.g., microspheres, thus offering the potential of a controlled

release, single dose hepatitis B vaccine. Copyright 2000 Wiley-Liss, Inc.

PMID: 11002244, UI: 20459469