Quantitative assessment of fibrosis in liver biopsies from patients with chronic hepatitis B

[Guest guest]

Liver International

Volume 24 Issue 6 Page 611 - December 2004

doi:10.1111/j.1478-3231.2004.0957.x

Clinical Studies

Liver International

Volume 24 Issue 6 Page 611 - December 2004

doi:10.1111/j.1478-3231.2004.0957.x

Clinical Studies

Quantitative assessment of fibrosis in liver biopsies from patients with

chronic hepatitis B

Yui Hui1, Choong-Tsek Liew2, Minnie Yin-Yin Go1, Angel Mei-Ling Chim1,

Henry Lik-Yuen Chan1, Wai-Yee Leung1 and ph Jao-Yiu Sung1

Abstract: Background/aim: Accurate histological assessment of liver fibrosis

is essential in the management of chronic hepatitis B (CHB). Although

semi-quantitative scoring systems describe well the pathological patterns of

hepatic structure, they produce fibrosis evaluation that is not very

precise. Image analysis or morphometry has the theoretical advantage of

providing truly quantitative data.

Patients and methods: The present study aimed at validating a new image

analysis system, Bioquant Nova Prime, in estimating collagen content in

liver biopsy samples from patients with CHB. The biopsies were stained with

picrosirius red and the areas of collagen were measured. The results were

correlated with laboratory parameters and Ishak modified histological

scores. Discriminative reliability of morphometry was determined using

receiver operating characteristics (ROC) analysis.

Results: There was excellent interobserver agreement (r=0.840.94, P<0.01) in

the morphometric analysis. Significant correlations between the quantitative

morphometric data and the semi-quantitative score (Spearman's r=0.680.78,

P<0.001) were also demonstrated. Excellent discriminative power of

morphometry in differentiating mild from advanced fibrosis and cirrhosis

from absence of cirrhosis was shown by the ROC analysis.

Conclusions: Our results validated the use of Bioquant Nova Prime in

estimating collagen content in liver biopsies. We showed that morphometry is

a sensitive method of liver fibrosis quantification in CHB and complements

semi-quantitative histological scoring system. This tool, with its reliable

intraassay variability, could be of special value in assessing histological

response to treatment after anti-viral or anti-fibrotic therapy.

Yui Hui1, Choong-Tsek Liew2, Minnie Yin-Yin Go1, Angel Mei-Ling Chim1,

Henry Lik-Yuen Chan1, Wai-Yee Leung1 and ph Jao-Yiu Sung1

Abstract: Background/aim: Accurate histological assessment of liver fibrosis

is essential in the management of chronic hepatitis B (CHB). Although

semi-quantitative scoring systems describe well the pathological patterns of

hepatic structure, they produce fibrosis evaluation that is not very

precise. Image analysis or morphometry has the theoretical advantage of

providing truly quantitative data.

Patients and methods: The present study aimed at validating a new image

analysis system, Bioquant Nova Prime, in estimating collagen content in

liver biopsy samples from patients with CHB. The biopsies were stained with

picrosirius red and the areas of collagen were measured. The results were

correlated with laboratory parameters and Ishak modified histological

scores. Discriminative reliability of morphometry was determined using

receiver operating characteristics (ROC) analysis.

Results: There was excellent interobserver agreement (r=0.840.94, P<0.01) in

the morphometric analysis. Significant correlations between the quantitative

morphometric data and the semi-quantitative score (Spearman's r=0.680.78,

P<0.001) were also demonstrated. Excellent discriminative power of

morphometry in differentiating mild from advanced fibrosis and cirrhosis

from absence of cirrhosis was shown by the ROC analysis.

Conclusions: Our results validated the use of Bioquant Nova Prime in

estimating collagen content in liver biopsies. We showed that morphometry is

a sensitive method of liver fibrosis quantification in CHB and complements

semi-quantitative histological scoring system. This tool, with its reliable

intraassay variability, could be of special value in assessing histological

response to treatment after anti-viral or anti-fibrotic therapy.

[Guest guest]

Liver International

Volume 24 Issue 6 Page 611 - December 2004

doi:10.1111/j.1478-3231.2004.0957.x

Clinical Studies

Liver International

Volume 24 Issue 6 Page 611 - December 2004

doi:10.1111/j.1478-3231.2004.0957.x

Clinical Studies

Quantitative assessment of fibrosis in liver biopsies from patients with

chronic hepatitis B

Yui Hui1, Choong-Tsek Liew2, Minnie Yin-Yin Go1, Angel Mei-Ling Chim1,

Henry Lik-Yuen Chan1, Wai-Yee Leung1 and ph Jao-Yiu Sung1

Abstract: Background/aim: Accurate histological assessment of liver fibrosis

is essential in the management of chronic hepatitis B (CHB). Although

semi-quantitative scoring systems describe well the pathological patterns of

hepatic structure, they produce fibrosis evaluation that is not very

precise. Image analysis or morphometry has the theoretical advantage of

providing truly quantitative data.

Patients and methods: The present study aimed at validating a new image

analysis system, Bioquant Nova Prime, in estimating collagen content in

liver biopsy samples from patients with CHB. The biopsies were stained with

picrosirius red and the areas of collagen were measured. The results were

correlated with laboratory parameters and Ishak modified histological

scores. Discriminative reliability of morphometry was determined using

receiver operating characteristics (ROC) analysis.

Results: There was excellent interobserver agreement (r=0.840.94, P<0.01) in

the morphometric analysis. Significant correlations between the quantitative

morphometric data and the semi-quantitative score (Spearman's r=0.680.78,

P<0.001) were also demonstrated. Excellent discriminative power of

morphometry in differentiating mild from advanced fibrosis and cirrhosis

from absence of cirrhosis was shown by the ROC analysis.

Conclusions: Our results validated the use of Bioquant Nova Prime in

estimating collagen content in liver biopsies. We showed that morphometry is

a sensitive method of liver fibrosis quantification in CHB and complements

semi-quantitative histological scoring system. This tool, with its reliable

intraassay variability, could be of special value in assessing histological

response to treatment after anti-viral or anti-fibrotic therapy.

Yui Hui1, Choong-Tsek Liew2, Minnie Yin-Yin Go1, Angel Mei-Ling Chim1,

Henry Lik-Yuen Chan1, Wai-Yee Leung1 and ph Jao-Yiu Sung1

Abstract: Background/aim: Accurate histological assessment of liver fibrosis

is essential in the management of chronic hepatitis B (CHB). Although

semi-quantitative scoring systems describe well the pathological patterns of

hepatic structure, they produce fibrosis evaluation that is not very

precise. Image analysis or morphometry has the theoretical advantage of

providing truly quantitative data.

Patients and methods: The present study aimed at validating a new image

analysis system, Bioquant Nova Prime, in estimating collagen content in

liver biopsy samples from patients with CHB. The biopsies were stained with

picrosirius red and the areas of collagen were measured. The results were

correlated with laboratory parameters and Ishak modified histological

scores. Discriminative reliability of morphometry was determined using

receiver operating characteristics (ROC) analysis.

Results: There was excellent interobserver agreement (r=0.840.94, P<0.01) in

the morphometric analysis. Significant correlations between the quantitative

morphometric data and the semi-quantitative score (Spearman's r=0.680.78,

P<0.001) were also demonstrated. Excellent discriminative power of

morphometry in differentiating mild from advanced fibrosis and cirrhosis

from absence of cirrhosis was shown by the ROC analysis.

Conclusions: Our results validated the use of Bioquant Nova Prime in

estimating collagen content in liver biopsies. We showed that morphometry is

a sensitive method of liver fibrosis quantification in CHB and complements

semi-quantitative histological scoring system. This tool, with its reliable

intraassay variability, could be of special value in assessing histological

response to treatment after anti-viral or anti-fibrotic therapy.

[Guest guest]

Liver International

Volume 24 Issue 6 Page 611 - December 2004

doi:10.1111/j.1478-3231.2004.0957.x

Clinical Studies

Liver International

Volume 24 Issue 6 Page 611 - December 2004

doi:10.1111/j.1478-3231.2004.0957.x

Clinical Studies

Quantitative assessment of fibrosis in liver biopsies from patients with

chronic hepatitis B

Yui Hui1, Choong-Tsek Liew2, Minnie Yin-Yin Go1, Angel Mei-Ling Chim1,

Henry Lik-Yuen Chan1, Wai-Yee Leung1 and ph Jao-Yiu Sung1

Abstract: Background/aim: Accurate histological assessment of liver fibrosis

is essential in the management of chronic hepatitis B (CHB). Although

semi-quantitative scoring systems describe well the pathological patterns of

hepatic structure, they produce fibrosis evaluation that is not very

precise. Image analysis or morphometry has the theoretical advantage of

providing truly quantitative data.

Patients and methods: The present study aimed at validating a new image

analysis system, Bioquant Nova Prime, in estimating collagen content in

liver biopsy samples from patients with CHB. The biopsies were stained with

picrosirius red and the areas of collagen were measured. The results were

correlated with laboratory parameters and Ishak modified histological

scores. Discriminative reliability of morphometry was determined using

receiver operating characteristics (ROC) analysis.

Results: There was excellent interobserver agreement (r=0.840.94, P<0.01) in

the morphometric analysis. Significant correlations between the quantitative

morphometric data and the semi-quantitative score (Spearman's r=0.680.78,

P<0.001) were also demonstrated. Excellent discriminative power of

morphometry in differentiating mild from advanced fibrosis and cirrhosis

from absence of cirrhosis was shown by the ROC analysis.

Conclusions: Our results validated the use of Bioquant Nova Prime in

estimating collagen content in liver biopsies. We showed that morphometry is

a sensitive method of liver fibrosis quantification in CHB and complements

semi-quantitative histological scoring system. This tool, with its reliable

intraassay variability, could be of special value in assessing histological

response to treatment after anti-viral or anti-fibrotic therapy.

Yui Hui1, Choong-Tsek Liew2, Minnie Yin-Yin Go1, Angel Mei-Ling Chim1,

Henry Lik-Yuen Chan1, Wai-Yee Leung1 and ph Jao-Yiu Sung1

Abstract: Background/aim: Accurate histological assessment of liver fibrosis

is essential in the management of chronic hepatitis B (CHB). Although

semi-quantitative scoring systems describe well the pathological patterns of

hepatic structure, they produce fibrosis evaluation that is not very

precise. Image analysis or morphometry has the theoretical advantage of

providing truly quantitative data.

Patients and methods: The present study aimed at validating a new image

analysis system, Bioquant Nova Prime, in estimating collagen content in

liver biopsy samples from patients with CHB. The biopsies were stained with

picrosirius red and the areas of collagen were measured. The results were

correlated with laboratory parameters and Ishak modified histological

scores. Discriminative reliability of morphometry was determined using

receiver operating characteristics (ROC) analysis.

Results: There was excellent interobserver agreement (r=0.840.94, P<0.01) in

the morphometric analysis. Significant correlations between the quantitative

morphometric data and the semi-quantitative score (Spearman's r=0.680.78,

P<0.001) were also demonstrated. Excellent discriminative power of

morphometry in differentiating mild from advanced fibrosis and cirrhosis

from absence of cirrhosis was shown by the ROC analysis.

Conclusions: Our results validated the use of Bioquant Nova Prime in

estimating collagen content in liver biopsies. We showed that morphometry is

a sensitive method of liver fibrosis quantification in CHB and complements

semi-quantitative histological scoring system. This tool, with its reliable

intraassay variability, could be of special value in assessing histological

response to treatment after anti-viral or anti-fibrotic therapy.

[Guest guest]

Liver International

Volume 24 Issue 6 Page 611 - December 2004

doi:10.1111/j.1478-3231.2004.0957.x

Clinical Studies

Liver International

Volume 24 Issue 6 Page 611 - December 2004

doi:10.1111/j.1478-3231.2004.0957.x

Clinical Studies

Quantitative assessment of fibrosis in liver biopsies from patients with

chronic hepatitis B

Yui Hui1, Choong-Tsek Liew2, Minnie Yin-Yin Go1, Angel Mei-Ling Chim1,

Henry Lik-Yuen Chan1, Wai-Yee Leung1 and ph Jao-Yiu Sung1

Abstract: Background/aim: Accurate histological assessment of liver fibrosis

is essential in the management of chronic hepatitis B (CHB). Although

semi-quantitative scoring systems describe well the pathological patterns of

hepatic structure, they produce fibrosis evaluation that is not very

precise. Image analysis or morphometry has the theoretical advantage of

providing truly quantitative data.

Patients and methods: The present study aimed at validating a new image

analysis system, Bioquant Nova Prime, in estimating collagen content in

liver biopsy samples from patients with CHB. The biopsies were stained with

picrosirius red and the areas of collagen were measured. The results were

correlated with laboratory parameters and Ishak modified histological

scores. Discriminative reliability of morphometry was determined using

receiver operating characteristics (ROC) analysis.

Results: There was excellent interobserver agreement (r=0.840.94, P<0.01) in

the morphometric analysis. Significant correlations between the quantitative

morphometric data and the semi-quantitative score (Spearman's r=0.680.78,

P<0.001) were also demonstrated. Excellent discriminative power of

morphometry in differentiating mild from advanced fibrosis and cirrhosis

from absence of cirrhosis was shown by the ROC analysis.

Conclusions: Our results validated the use of Bioquant Nova Prime in

estimating collagen content in liver biopsies. We showed that morphometry is

a sensitive method of liver fibrosis quantification in CHB and complements

semi-quantitative histological scoring system. This tool, with its reliable

intraassay variability, could be of special value in assessing histological

response to treatment after anti-viral or anti-fibrotic therapy.

Yui Hui1, Choong-Tsek Liew2, Minnie Yin-Yin Go1, Angel Mei-Ling Chim1,

Henry Lik-Yuen Chan1, Wai-Yee Leung1 and ph Jao-Yiu Sung1

Abstract: Background/aim: Accurate histological assessment of liver fibrosis

is essential in the management of chronic hepatitis B (CHB). Although

semi-quantitative scoring systems describe well the pathological patterns of

hepatic structure, they produce fibrosis evaluation that is not very

precise. Image analysis or morphometry has the theoretical advantage of

providing truly quantitative data.

Patients and methods: The present study aimed at validating a new image

analysis system, Bioquant Nova Prime, in estimating collagen content in

liver biopsy samples from patients with CHB. The biopsies were stained with

picrosirius red and the areas of collagen were measured. The results were

correlated with laboratory parameters and Ishak modified histological

scores. Discriminative reliability of morphometry was determined using

receiver operating characteristics (ROC) analysis.

Results: There was excellent interobserver agreement (r=0.840.94, P<0.01) in

the morphometric analysis. Significant correlations between the quantitative

morphometric data and the semi-quantitative score (Spearman's r=0.680.78,

P<0.001) were also demonstrated. Excellent discriminative power of

morphometry in differentiating mild from advanced fibrosis and cirrhosis

from absence of cirrhosis was shown by the ROC analysis.

Conclusions: Our results validated the use of Bioquant Nova Prime in

estimating collagen content in liver biopsies. We showed that morphometry is

a sensitive method of liver fibrosis quantification in CHB and complements

semi-quantitative histological scoring system. This tool, with its reliable

intraassay variability, could be of special value in assessing histological

response to treatment after anti-viral or anti-fibrotic therapy.