YMDD motif variants in inactive hepatitis B carriers detected by Inno-Lipa HBV D

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Journal of Gastroenterology and Hepatology

Volume 21 Page 1783 - December 2006

doi:10.1111/j.1440-1746.2006.04567.x

Volume 21 Issue 12

HEPATOLOGY

YMDD motif variants in inactive hepatitis B carriers detected by Inno-Lipa

HBV DR assay

Mesut Akarsu,* Aylin Sengonul, Ethem Tankurt,* Ayca Arzu Sayiner, Omer

Topalak,* Hale Akpinar* and Yusuf Hakan Abacioglu

Abstract

Introduction: Mutations of hepatitis B virus (HBV) polymerase, especially

occurring at the highly conserved YMDD region, are related to resistance to

lamivudine. Although these mutations are frequently secondary to lamivudine

use, they can also occur naturally. The aim of the present study was to

determine the prevalence of YMDD variants that exist naturally in patients

who are inactive HBV carriers.

Methods: Seventy-one adult inactive HBV carriers were studied. All of the

patients were confirmed to have maintained normal alanine aminotransferase

(ALT) values for one or more years by monitoring serum ALT levels at

3-monthly intervals. None of the patients received interferon or antiviral

agents. YMDD variants were analyzed by the HBV Drug Resistance Line Probe

assay (Inno-Lipa HBV-DR).

Results: YMDD variants were detected in 13 (18.3%) of the 71 anti-HBe

positive inactive HBV carriers. Of the 13 patients, 10 (76.9%) also had

accompanying L180M mutation. The combination of wild type and YMDD variant

HBV was present in 11 of 13 patients. In two patients, only YIDD and/or YVDD

variants plus L180M were detected without the presence of wild YMDD motif.

Conclusion: Naturally occurring YMDD motif variants were detected at a high

rate in a group of lamivudine-untreated inactive HBV carriers.

http://www.blackwell-synergy.com/doi/abs/10.1111/j.1440-1746.2006.04567.x

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[Guest guest]

Journal of Gastroenterology and Hepatology

Volume 21 Page 1783 - December 2006

doi:10.1111/j.1440-1746.2006.04567.x

Volume 21 Issue 12

HEPATOLOGY

YMDD motif variants in inactive hepatitis B carriers detected by Inno-Lipa

HBV DR assay

Mesut Akarsu,* Aylin Sengonul, Ethem Tankurt,* Ayca Arzu Sayiner, Omer

Topalak,* Hale Akpinar* and Yusuf Hakan Abacioglu

Abstract

Introduction: Mutations of hepatitis B virus (HBV) polymerase, especially

occurring at the highly conserved YMDD region, are related to resistance to

lamivudine. Although these mutations are frequently secondary to lamivudine

use, they can also occur naturally. The aim of the present study was to

determine the prevalence of YMDD variants that exist naturally in patients

who are inactive HBV carriers.

Methods: Seventy-one adult inactive HBV carriers were studied. All of the

patients were confirmed to have maintained normal alanine aminotransferase

(ALT) values for one or more years by monitoring serum ALT levels at

3-monthly intervals. None of the patients received interferon or antiviral

agents. YMDD variants were analyzed by the HBV Drug Resistance Line Probe

assay (Inno-Lipa HBV-DR).

Results: YMDD variants were detected in 13 (18.3%) of the 71 anti-HBe

positive inactive HBV carriers. Of the 13 patients, 10 (76.9%) also had

accompanying L180M mutation. The combination of wild type and YMDD variant

HBV was present in 11 of 13 patients. In two patients, only YIDD and/or YVDD

variants plus L180M were detected without the presence of wild YMDD motif.

Conclusion: Naturally occurring YMDD motif variants were detected at a high

rate in a group of lamivudine-untreated inactive HBV carriers.

http://www.blackwell-synergy.com/doi/abs/10.1111/j.1440-1746.2006.04567.x

_________________________________________________________________

Get FREE company branded e-mail accounts and business Web site from

Microsoft Office Live

http://clk.atdmt.com/MRT/go/mcrssaub0050001411mrt/direct/01/