Molecular characterization of hepatitis B virus strains circulating in Belgian patients co-infected with HIV and HBV: Overt and occult infection

[Guest guest]

J Med Virol. 2011 Nov;83(11):1876-84. doi: 10.1002/jmv.22174.

Molecular characterization of hepatitis B virus strains circulating in Belgian

patients co-infected with HIV and HBV: Overt and occult infection.

Pourkarim MR, Lemey P, Amini-Bavil-Olyaee S, Houspie L, Verbeeck J, Rahman M,

Maes P, Vanwijngaerden E, Nevens F, Van Ranst M.

Source

Laboratory of Clinical Virology, Rega Institute for Medical Research, Katholieke

Universiteit, Leuven, Belgium; Blood Transfusion Research Center, High Institute

for Research and Education in Transfusion Medicine, Tehran, Iran.

Abstract

Hepatitis B virus (HBV) and human immunodeficiency virus (HIV) have similar

transmission routes, implying that patients infected with HIV are at particular

risk for HBV infection. Patients who are co-infected with HIV and HBV progress

more rapidly to end-stage liver disease and different HBV genotypes may have a

distinct impact on disease progression. One hundred ninety-one anti-HBc-positive

sera from Belgian patients co-infected with HIV and HBV were collected during

1998-2008. Full-length HBV genomes as well as large S or partial S genes were

amplified and their molecular evolutionary history was analyzed. Clinically, 30

(65.8%) patients were categorized as " overt infection " and 16 (34.7%) cases were

categorized as " occult infection. " Five distinct HBV genotypes comprising A

(69.6%), E (19.6%), followed by D, C, and G were detected. HBV genotype A was

observed in all clinical groups and in patients with varying ethnical

background. HBV genotype E could be detected in African patients who were mostly

infected by heterosexual contacts. Several clinically important mutations at the

HBs major hydrophilic region were detected in the new isolates but with no

significant difference between occult and overt infection. The high prevalence

of HBV genotype A in overt and occult cases, and in particular the detection of

certain HBV subgenotypes in patients co-infected with HIV and HBV that carry

diagnostic escape mutations, may provide useful information for national

guidelines for prophylaxis and treatment.

J. Med. Virol. 83:1876-1884, 2011. © 2011 Wiley-Liss, Inc.

Copyright © 2011 Wiley-Liss, Inc.

PMID: 21915860 [PubMed - in process]

[Guest guest]

J Med Virol. 2011 Nov;83(11):1876-84. doi: 10.1002/jmv.22174.

Molecular characterization of hepatitis B virus strains circulating in Belgian

patients co-infected with HIV and HBV: Overt and occult infection.

Pourkarim MR, Lemey P, Amini-Bavil-Olyaee S, Houspie L, Verbeeck J, Rahman M,

Maes P, Vanwijngaerden E, Nevens F, Van Ranst M.

Source

Laboratory of Clinical Virology, Rega Institute for Medical Research, Katholieke

Universiteit, Leuven, Belgium; Blood Transfusion Research Center, High Institute

for Research and Education in Transfusion Medicine, Tehran, Iran.

Abstract

Hepatitis B virus (HBV) and human immunodeficiency virus (HIV) have similar

transmission routes, implying that patients infected with HIV are at particular

risk for HBV infection. Patients who are co-infected with HIV and HBV progress

more rapidly to end-stage liver disease and different HBV genotypes may have a

distinct impact on disease progression. One hundred ninety-one anti-HBc-positive

sera from Belgian patients co-infected with HIV and HBV were collected during

1998-2008. Full-length HBV genomes as well as large S or partial S genes were

amplified and their molecular evolutionary history was analyzed. Clinically, 30

(65.8%) patients were categorized as " overt infection " and 16 (34.7%) cases were

categorized as " occult infection. " Five distinct HBV genotypes comprising A

(69.6%), E (19.6%), followed by D, C, and G were detected. HBV genotype A was

observed in all clinical groups and in patients with varying ethnical

background. HBV genotype E could be detected in African patients who were mostly

infected by heterosexual contacts. Several clinically important mutations at the

HBs major hydrophilic region were detected in the new isolates but with no

significant difference between occult and overt infection. The high prevalence

of HBV genotype A in overt and occult cases, and in particular the detection of

certain HBV subgenotypes in patients co-infected with HIV and HBV that carry

diagnostic escape mutations, may provide useful information for national

guidelines for prophylaxis and treatment.

J. Med. Virol. 83:1876-1884, 2011. © 2011 Wiley-Liss, Inc.

Copyright © 2011 Wiley-Liss, Inc.

PMID: 21915860 [PubMed - in process]