Thermal stability and inactivation of hepatitis C virus grown in cell culture

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http://7thspace.com/headlines/335485/thermal_stability_and_inactivation_of_hepat\

itis_c_virus_grown_in_cell_culture.html Thermal stability and inactivation of

hepatitis C virus grown in cell culture Hepatitis C virus (HCV) is a blood-borne

flavivirus that infects many millions of people worldwide. Relatively little is

known, however, concerning the stability of HCV and reliable procedures for

inactivating this virus.Methods: In the current study, the thermostability of

cell culture-derived HCV (HCVcc, JFH-1 strain) under different environmental

temperatures (37degreesC, room temperature, and 4degreesC) and the ability of

heat, UVC light irradiation, and aldehyde and detergent treatments to inactivate

HCVcc were evaluated.The infectious titers of treated viral samples were

determined by focus-forming unit (FFU) assay using an indirect

immunofluorescence assay for HCV NS3 in hepatoma Huh7-25-CD81 cells highly

permissive for HCVcc infection. MTT cytotoxicity assay was performed to

determine the concentrations of aldehydes or detergents at which they were no

longer cytotoxic.Results: HCVcc in culture medium was found to survive

37degreesC and room temperature (RT, 25 +/- 2degreesC) for 2 and 16 days,

respectively, while the virus was relatively stable at 4degreesC without drastic

loss of infectivity for at least 6 weeks.HCVcc in culture medium was sensitive

to heat and could be inactivated in 8 and 4 min when incubated at 60degreesC and

65degreesC, respectively. However, at 56degreesC, 40 min were required to

eliminate HCVcc infectivity.Addition of normal human serum to HCVcc did not

significantly alter viral stability at RT or its susceptibility to heat. UVC

light irradiation (wavelength = 253.7 nm) with an intensity of 450 muW/cm2

efficiently inactivated HCVcc within 2 min.Exposures to formaldehyde,

glutaraldehyde, ionic or nonionic detergents all destroyed HCVcc infectivity

effectively, regardless of whether the treatments were conducted in the presence

of cell culture medium or human serum.Conclusions: The results provide

quantitative evidence for the potential use of a variety of approaches for

inactivating HCV. The ability of HCVcc to survive ambient temperatures warrants

precautions in handling and disposing of objects and materials that may have

been contaminated with HCV.Author: Hongshuo SongJin LiShuang ShiLing YanHui

ZhuangKui LiCredits/Source: Virology Journal 2010, 7:40

[Guest guest]

http://7thspace.com/headlines/335485/thermal_stability_and_inactivation_of_hepat\

itis_c_virus_grown_in_cell_culture.html Thermal stability and inactivation of

hepatitis C virus grown in cell culture Hepatitis C virus (HCV) is a blood-borne

flavivirus that infects many millions of people worldwide. Relatively little is

known, however, concerning the stability of HCV and reliable procedures for

inactivating this virus.Methods: In the current study, the thermostability of

cell culture-derived HCV (HCVcc, JFH-1 strain) under different environmental

temperatures (37degreesC, room temperature, and 4degreesC) and the ability of

heat, UVC light irradiation, and aldehyde and detergent treatments to inactivate

HCVcc were evaluated.The infectious titers of treated viral samples were

determined by focus-forming unit (FFU) assay using an indirect

immunofluorescence assay for HCV NS3 in hepatoma Huh7-25-CD81 cells highly

permissive for HCVcc infection. MTT cytotoxicity assay was performed to

determine the concentrations of aldehydes or detergents at which they were no

longer cytotoxic.Results: HCVcc in culture medium was found to survive

37degreesC and room temperature (RT, 25 +/- 2degreesC) for 2 and 16 days,

respectively, while the virus was relatively stable at 4degreesC without drastic

loss of infectivity for at least 6 weeks.HCVcc in culture medium was sensitive

to heat and could be inactivated in 8 and 4 min when incubated at 60degreesC and

65degreesC, respectively. However, at 56degreesC, 40 min were required to

eliminate HCVcc infectivity.Addition of normal human serum to HCVcc did not

significantly alter viral stability at RT or its susceptibility to heat. UVC

light irradiation (wavelength = 253.7 nm) with an intensity of 450 muW/cm2

efficiently inactivated HCVcc within 2 min.Exposures to formaldehyde,

glutaraldehyde, ionic or nonionic detergents all destroyed HCVcc infectivity

effectively, regardless of whether the treatments were conducted in the presence

of cell culture medium or human serum.Conclusions: The results provide

quantitative evidence for the potential use of a variety of approaches for

inactivating HCV. The ability of HCVcc to survive ambient temperatures warrants

precautions in handling and disposing of objects and materials that may have

been contaminated with HCV.Author: Hongshuo SongJin LiShuang ShiLing YanHui

ZhuangKui LiCredits/Source: Virology Journal 2010, 7:40