Association of polymorphisms of programmed cell death–1 gene with chronic hepatitis B virus infection

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Human Immunology

Volume 71, Issue 12, December 2010, Pages 1209-1213

Association of polymorphisms of programmed cell death–1 gene with chronic

hepatitis B virus infection

References and further reading may be available for this article. To view

references and further reading you must purchase this article.

Guoyu Zhanga, Zhengwen Liua, , , Shaoqiong Duana, Qunying Hana, Zhu Lia, Yi Lvb,

Jinghong Chenc, Sai Loua and Na Lia

a Department of Infectious Diseases, First Affiliated Hospital, School of

Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi Province, People's Republic

of China

b Department of Hepatobiliary Surgery, First Affiliated Hospital, School of

Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi Province, People's Republic

of China

c Institute of Endemic Diseases, School of Medicine, Xi'an Jiaotong University,

Key Laboratory of Environment and Genes related to Diseases, Ministry of

Education, Xi'an, Shaanxi, People's Republic of China

Received 4 June 2010; accepted 30 August 2010. Available online 15 September

2010. Available online 15 September 2010.

Abstract

Programmed cell death–1 (PD-1) plays a critical role in regulating T-cell

function during hepatitis B virus (HBV) infection. The present study

investigated the relationships between the polymorphisms of the PD-1 gene and

the susceptibility to chronic HBV infection. Single nucleotide polymorphisms

(SNPs) in PD-1 gene at positions −606G/A (PD-1.1) and +8669 G/A (PD-1.6) were

analyzed by bidirectional PCR amplification of specific alleles (Bi-PASA) in 198

chronic HBV patients and 280 controls. Although the genotype and allele

frequencies of PD-1.1 were not different between chronic HBV patients and

controls, the genotype and allele frequencies of PD-1.6 were significantly

different. PD-1.6 GG genotype and the combination of genotypes with G allele

were less frequent in HBV patients than in controls (p = 0.007 and p = 0.031,

respectively). The allele G was also less frequent in patients than in controls

(p = 0.006). Haplotype PD-1.1G/PD-1.6G was less frequent in patients than in

controls (p = 0.001). Cirrhosis patients had a lower frequency of PD-1.6 G

allele compared with controls (p = 0.007). Our findings, firstly reporting the

association between PD-1 polymorphism and HBV infection, suggest that PD-1 gene

may be one of the genes predisposing to chronic HBV infection and disease

progression.

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Human Immunology

Volume 71, Issue 12, December 2010, Pages 1209-1213

Association of polymorphisms of programmed cell death–1 gene with chronic

hepatitis B virus infection

References and further reading may be available for this article. To view

references and further reading you must purchase this article.

Guoyu Zhanga, Zhengwen Liua, , , Shaoqiong Duana, Qunying Hana, Zhu Lia, Yi Lvb,

Jinghong Chenc, Sai Loua and Na Lia

a Department of Infectious Diseases, First Affiliated Hospital, School of

Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi Province, People's Republic

of China

b Department of Hepatobiliary Surgery, First Affiliated Hospital, School of

Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi Province, People's Republic

of China

c Institute of Endemic Diseases, School of Medicine, Xi'an Jiaotong University,

Key Laboratory of Environment and Genes related to Diseases, Ministry of

Education, Xi'an, Shaanxi, People's Republic of China

Received 4 June 2010; accepted 30 August 2010. Available online 15 September

2010. Available online 15 September 2010.

Abstract

Programmed cell death–1 (PD-1) plays a critical role in regulating T-cell

function during hepatitis B virus (HBV) infection. The present study

investigated the relationships between the polymorphisms of the PD-1 gene and

the susceptibility to chronic HBV infection. Single nucleotide polymorphisms

(SNPs) in PD-1 gene at positions −606G/A (PD-1.1) and +8669 G/A (PD-1.6) were

analyzed by bidirectional PCR amplification of specific alleles (Bi-PASA) in 198

chronic HBV patients and 280 controls. Although the genotype and allele

frequencies of PD-1.1 were not different between chronic HBV patients and

controls, the genotype and allele frequencies of PD-1.6 were significantly

different. PD-1.6 GG genotype and the combination of genotypes with G allele

were less frequent in HBV patients than in controls (p = 0.007 and p = 0.031,

respectively). The allele G was also less frequent in patients than in controls

(p = 0.006). Haplotype PD-1.1G/PD-1.6G was less frequent in patients than in

controls (p = 0.001). Cirrhosis patients had a lower frequency of PD-1.6 G

allele compared with controls (p = 0.007). Our findings, firstly reporting the

association between PD-1 polymorphism and HBV infection, suggest that PD-1 gene

may be one of the genes predisposing to chronic HBV infection and disease

progression.