Amino acid substitutions in the S region of hepatitis B virus in sera from patie

July 6, 2007

Hepatology Research 37 (9), 731–739.

doi:10.1111/j.1872-034X.2007.00120.x

Abstract

Original Article

Amino acid substitutions in the S region of hepatitis B virus in sera from

patients with acute hepatitis

Junko Aono,1,3Departments of 1Infectious Diseases and 3Faculty of

Pharmaceutical Sciences, Teikyo Heisei University, Chiba, Hiroshi

Yotsuyanagi,1Departments of 1Infectious Diseases and Dr Hiroshi Yotsuyanagi,

Department of Infectious Diseases, Internal Medicine, University of Tokyo,

7-3-1 Hongo, Bunkyo, Tokyo 113-8655, Japan. Email: hyotsu-tky@...

Hideyuki Miyoshi,22Gastroenterology, Internal Medicine, University of Tokyo,

Takeya Tsutsumi,1Departments of 1Infectious Diseases and Hajime

Fujie,22Gastroenterology, Internal Medicine, University of Tokyo, Yoshizumi

Shintani,1Departments of 1Infectious Diseases and Kyoji Moriya,1Departments

of 1Infectious Diseases and Chiaki Okuse44Division of Gastroenterology and

Hepatology, Department of Internal Medicine, St. nna University School

of Medicine, Kawasaki, Japan , Michihiro Suzuki,44Division of

Gastroenterology and Hepatology, Department of Internal Medicine, St.

nna University School of Medicine, Kawasaki, Japan Kiyomi

Yasuda,55Center for Liver Diseases, Seizankai Kiyokawa Hospital, Tokyo,

Shiro Iino55Center for Liver Diseases, Seizankai Kiyokawa Hospital, Tokyo,

and Kazuhiko Koike1Departments of 1Infectious Diseases and Departments of

1Infectious Diseases and 2Gastroenterology, Internal Medicine, University of

Tokyo, 5Center for Liver Diseases, Seizankai Kiyokawa Hospital, Tokyo,

3Faculty of Pharmaceutical Sciences, Teikyo Heisei University, Chiba,

4Division of Gastroenterology and Hepatology, Department of Internal

Medicine, St. nna University School of Medicine, Kawasaki, Japan

Dr Hiroshi Yotsuyanagi, Department of Infectious Diseases, Internal

Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-8655, Japan.

Email: hyotsu-tky@...

Abstract

Background: An increase in the number of acute hepatitis patients with

hepatitis B virus (HBV) of non-indigenous genotypes may reduce the efficacy

of vaccination against HBV.

Methods: We have determined the amino acid (aa) sequences in the major

hydrophilic region (MHR) in the S region of HBV in patients with acute

hepatitis B and compared those with the ones from HBV strains used for the

production of HBV vaccines commercially available in Japan.

Results: Of 48 patients studied, 11 were infected with genotype A, 11 with

genotype B and 26 with genotype C HBV. The aa sequences of the nine genotype

A isolates were the same as the aa sequence of J02205 which is used for the

production of one of the commercially available recombinant vaccines. The aa

sequences of the 11 genotype B isolates differed from the aa sequence of

J02205 in two or three amino acids. Of the26 genotype C isolates, 22 had the

same aa sequence as X01587 which is used for the production of another

recombinant vaccine. The remaining genotype C isolates had aa substitutions

at aa131, which have a potential to alter the hydropathy and the

three-dimensional structure of the MHR. The differences among the three

current HBV vaccines in aa sequences in the MHR theoretically alter the

hydropathy and three-dimensional structure.

Conclusion: Our results suggest that the transmission of HBV isolates with

different genotypes or with aa substitutions in the MHR might reduce the

efficacy of currently available HBV vaccines in the protection of HBV

infections.

http://www.blackwell-synergy.com/doi/abs/10.1111/j.1872-034X.2007.00120.x

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July 6, 2007