Variations in the functional domain of basal core promoter of hepatitis B virus among Eastern Indian patients with prevalence of genotypes A, C, and D among the same ethnic population

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J Med Virol. 2011 Feb;83(2):253-60.

Variations in the functional domain of basal core promoter of hepatitis B virus

among Eastern Indian patients with prevalence of genotypes A, C, and D among the

same ethnic population.

Biswas A, Banerjee A, Chandra PK, Datta S, Panigrahi R, Dutta D, De BK, Pal M,

Guha SK, Chakrabarti S, Chakravarty R.

ICMR Virus Unit, Kolkata, West Bengal, India.

Abstract

Mutations in the basal core promoter (BCP) and precore (PC) regions are

associated with persistent and intermittently high hepatitis B virus (HBV)

replication in several patients. The variability in the functional domains of

BCP and PC region of HBV and their association with disease progression and

clinical outcome were assessed in Eastern India, an unique region where three

HBV genotypes, A, D, and C are prevalent among the same ethnic group. PCR

amplification and direct sequencing of BCP and PC region was done on sera

obtained from 130 HBsAg positive subjects with different clinical presentations.

Associations of the apparent risk factors with clinical advancement were

evaluated by statistical methods including multiple logistic regression analyses

(MLR). HBV genotype A was present in 33.08%, C in 25.38%, and D in 41.54% cases.

Genotypes A and C were associated with higher rate of T1762/A1764 mutations than

the most predominant genotype D. HBeAg negative state was associated with

considerably higher rate of C1753 mutation. T1762/A1764 along with C1753 was

common among cirrhosis and T1762/A1764 without C1753 was frequent among chronic

liver disease cases. No significant association was found between A1896 point

mutation and clinical status. Multivariate analysis revealed that T1762/A1764

double mutation, HBV/A, age ™25 years, C1753 and A1899 were critical factors for

clinical advancement while age ™25 years and C1753 as significant predictor for

cirrhosis in comparison with chronic liver disease. In conclusion, the analysis

of the BCP variability may help in monitoring the progression towards advanced

liver disease in Eastern Indian patients.

J. Med. Virol. 83:253-260, 2011. ¿ 2010 Wiley-Liss, Inc.

PMID: 21181919 [PubMed - in process]

[Guest guest]

J Med Virol. 2011 Feb;83(2):253-60.

Variations in the functional domain of basal core promoter of hepatitis B virus

among Eastern Indian patients with prevalence of genotypes A, C, and D among the

same ethnic population.

Biswas A, Banerjee A, Chandra PK, Datta S, Panigrahi R, Dutta D, De BK, Pal M,

Guha SK, Chakrabarti S, Chakravarty R.

ICMR Virus Unit, Kolkata, West Bengal, India.

Abstract

Mutations in the basal core promoter (BCP) and precore (PC) regions are

associated with persistent and intermittently high hepatitis B virus (HBV)

replication in several patients. The variability in the functional domains of

BCP and PC region of HBV and their association with disease progression and

clinical outcome were assessed in Eastern India, an unique region where three

HBV genotypes, A, D, and C are prevalent among the same ethnic group. PCR

amplification and direct sequencing of BCP and PC region was done on sera

obtained from 130 HBsAg positive subjects with different clinical presentations.

Associations of the apparent risk factors with clinical advancement were

evaluated by statistical methods including multiple logistic regression analyses

(MLR). HBV genotype A was present in 33.08%, C in 25.38%, and D in 41.54% cases.

Genotypes A and C were associated with higher rate of T1762/A1764 mutations than

the most predominant genotype D. HBeAg negative state was associated with

considerably higher rate of C1753 mutation. T1762/A1764 along with C1753 was

common among cirrhosis and T1762/A1764 without C1753 was frequent among chronic

liver disease cases. No significant association was found between A1896 point

mutation and clinical status. Multivariate analysis revealed that T1762/A1764

double mutation, HBV/A, age ™25 years, C1753 and A1899 were critical factors for

clinical advancement while age ™25 years and C1753 as significant predictor for

cirrhosis in comparison with chronic liver disease. In conclusion, the analysis

of the BCP variability may help in monitoring the progression towards advanced

liver disease in Eastern Indian patients.

J. Med. Virol. 83:253-260, 2011. ¿ 2010 Wiley-Liss, Inc.

PMID: 21181919 [PubMed - in process]