Donor origin of de novo hepatocellular carcinoma in hepatic allografts1

[Guest guest]

Transplantation 2003; 76(11):1625-1627

Donor origin of de novo hepatocellular carcinoma in hepatic allografts1

Peer Flemming 2 5; Hans L. Tillmann 3; Hannelore Barg-Hock 4; Wolfram

Kleeberger 2; P. Manns 3; Juergen Klempnauer 4; Hans H. Kreipe 2

Background.

Hepatocellular carcinomas (HCC) that originate de novo in liver transplants

without preceding HCC in the explanted organ have only rarely been reported.

Because recent data demonstrated a mixed hepatocellular chimerism caused by

the integration of host-derived stem cells, a study was conducted on the

origin of tumor cells in de novo HCC.

Methods.

From two cases of de novo HCC arising in liver transplants after hepatitis B

reinfection, tumor cells and nonneoplastic liver cells from the patient's

own liver and donor liver were isolated by laser microdissection and highly

polymorphic short tandem DNA repeats (STR) were investigated.

Results.

Isolated tumor cells revealed donor-specific STR genotypes that could

clearly be discriminated from the genotype of the host.

Conclusions.

Hepatitis B virus-associated de novo HCC in liver transplants is of donor

but not host origin. The new technique described here can also discriminate

between true recurrence of the original tumor and new recipient tumors.

1 Supported by Deutsche Forschungsgemeinschaft grant SFB 265 C11.

2 Institut für Pathologie, Medizinische Hochschule Hannover, Hannover,

Germany.

3 Abteilung Gastroenterologie, Hepatologie und Endokrinologie, Medizinische

Hochschule Hannover, Hannover, Germany.

4 Klinik fuer Viszeral-und Transplantationschirurgie, Medizinische

Hochschule Hannover, Hannover, Germany.

5 Address correspondence to: Peer Flemming, M.D., Institut für Pathologie,

Medizinische Hochschule Hannover, Carl-Neuberg-Strasse 1, D-30625 Hannover,

Germany. E-mail: flemming.peer@ mh-hannover.de.

Received 29 January 2003.

Accepted 19 February 2003

[Guest guest]

Transplantation 2003; 76(11):1625-1627

Donor origin of de novo hepatocellular carcinoma in hepatic allografts1

Peer Flemming 2 5; Hans L. Tillmann 3; Hannelore Barg-Hock 4; Wolfram

Kleeberger 2; P. Manns 3; Juergen Klempnauer 4; Hans H. Kreipe 2

Background.

Hepatocellular carcinomas (HCC) that originate de novo in liver transplants

without preceding HCC in the explanted organ have only rarely been reported.

Because recent data demonstrated a mixed hepatocellular chimerism caused by

the integration of host-derived stem cells, a study was conducted on the

origin of tumor cells in de novo HCC.

Methods.

From two cases of de novo HCC arising in liver transplants after hepatitis B

reinfection, tumor cells and nonneoplastic liver cells from the patient's

own liver and donor liver were isolated by laser microdissection and highly

polymorphic short tandem DNA repeats (STR) were investigated.

Results.

Isolated tumor cells revealed donor-specific STR genotypes that could

clearly be discriminated from the genotype of the host.

Conclusions.

Hepatitis B virus-associated de novo HCC in liver transplants is of donor

but not host origin. The new technique described here can also discriminate

between true recurrence of the original tumor and new recipient tumors.

1 Supported by Deutsche Forschungsgemeinschaft grant SFB 265 C11.

2 Institut für Pathologie, Medizinische Hochschule Hannover, Hannover,

Germany.

3 Abteilung Gastroenterologie, Hepatologie und Endokrinologie, Medizinische

Hochschule Hannover, Hannover, Germany.

4 Klinik fuer Viszeral-und Transplantationschirurgie, Medizinische

Hochschule Hannover, Hannover, Germany.

5 Address correspondence to: Peer Flemming, M.D., Institut für Pathologie,

Medizinische Hochschule Hannover, Carl-Neuberg-Strasse 1, D-30625 Hannover,

Germany. E-mail: flemming.peer@ mh-hannover.de.

Received 29 January 2003.

Accepted 19 February 2003