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Gene expression profile of Li23, a new human hepatoma cell line that enables robust hepatitis C virus replication: Comparison with HuH-7 and other hepatic cell lines

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http://onlinelibrary.wiley.com/doi/10.1111/j.1872-034X.2010.00730.x/abstract

Gene expression profile of Li23, a new human hepatoma cell line that enables

robust hepatitis C virus replication: Comparison with HuH-7 and other hepatic

cell lines

Kyoko Mori†, Masanori Ikeda, Yasuo Ariumi, Nobuyuki Kato†Article first

published online: 7 OCT 2010

DOI: 10.1111/j.1872-034X.2010.00730.x

© 2010 The Japan Society of Hepatology

Issue

Hepatology Research

Volume 40, Issue 12, pages 1248–1253, December 2010

Aim:  Human hepatoma cell line HuH-7-derived cells are currently the only cell

culture system used for robust hepatitis C virus (HCV) replication. We recently

found a new human hepatoma cell line, Li23, that enables robust HCV replication.

Although both cell lines had similar liver-specific expression profiles, the

overall profile of Li23 seemed to differ considerably from that of HuH-7. To

understand this difference, the expression profile of Li23 cells was further

characterized by a comparison with that of HuH-7 cells.

Methods:  cDNA microarray analysis using Li23 and HuH-7 cells was performed.

Li23-derived ORL8c cells and HuH-7-derived RSc cells, in which HCV could infect

and efficiently replicate, were also used for the microarray analysis. For the

comparative analysis by reverse transcription polymerase chain reaction

(RT–PCR), human hepatoma cell lines (HuH-6, HepG2, HLE, HLF and PLC/PRF/5) and

immortalized hepatocyte cell line (PH5CH8) were also used.

Results:  Microarray analysis of Li23 versus HuH-7 cells selected 80 probes to

represent highly expressed genes that have ratios of more than 30 (Li23/HuH-7)

or 20 (HuH-7/Li23). Among them, 17 known genes were picked up for further

analysis. The expression levels of most of these genes in Li23 and HuH-7 cells

were retained in ORL8c and RSc cells, respectively. Comparative analysis by

RT–PCR using several other hepatic cell lines resulted in the classification

of 17 genes into three types, and identified three genes showing Li23-specific

expression profiles.

Conclusion:  Li23 is a new hepatoma cell line whose expression profile is

distinct from those of frequently used hepatic cell lines.

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