YMDD Mutations and Genotypes of Hepatitis B Virus in Northern China.

March 8, 2006

Jpn J Infect Dis. 2006 Feb;59(1):42-5.

Li D, Gu HX, Zhang SY, Zhong ZH, Zhuang M, Hattori T.

Department of Microbiology and Research Center of The Second Affiliated

Hospital, Harbin Medical University, Harbin, China.

hattori.t@....

The objective of this research was to determine the relationship between

YMDD mutations and the genotypes of hepatitis B virus (HBV) during

lamivudine treatment. HBV genotypes were determined by nested PCR with 6

pairs of HBV genotype-specific primers (A to F) in serum specimens from 142

hepatitis B patients receiving lamivudine antiviral therapy. YMDD mutations

were detected by fluorescent hybridization bioprobe PCR and melting curve

assay (FH-PCR-MC). Among 142 serum specimens, 13 samples were genotype B

(9.2%), 125 samples were genotype C (88%), 4 samples were genotype D (2.8%),

and 80 YMDD mutations were found. The YMDD mutation rates were 69.2 and

54.4% in genotype B and genotype C, respectively. There was no significant

difference in the YMDD mutation rate between genotypes B and C. Nine

genotype B sera with YMDD mutations were found, including 2 YIDD mutations

and 7 YVDD (M + V) mutations. Sixty-eight genotype C sera with YMDD

mutations were found, including 34 mutations I (M + I) and 17 mutations V (M

+ V). There was a significant difference in the YMDD mutation types between

genotypes B and C. Our results suggested that the YMDD mutation rate was

56.3% in patients treated with lamivudine for 2 - 4 years. YIDD was the main

mutation type. The YMDD mutation rate showed no significant difference

between HBV types B and C (P > 0.05), while the YMDD mutation types showed a

significant difference between HBV types B and C in Northern China

(chi-square test = 4.6, P < 0.05).

PMID: 16495633 [PubMed - in process]

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