Dark Field Microscopy and Electrification

[Guest guest]

Hi All

I have had my third Dark Field Microscope (DFM) blood exam yesterday and here is

the scope of my progress since 22.Jun.2009.

I was on juicing, minerals, homeopathic antifungals, intestinal cleaning remedy

etc. in a somewhat erratic manner due to being new to this healing stuff since

22.Jun.2009 when I started digging for information to fix what the pros would

not even identify.

My first DFM exam in Aug 2009 showed:

- Red blood cells clumped into necklaces of " coin " stacks. Very few loose red

blood cells present.

- Fair portion of red blood cells torn by bacteria.

- Clumps of fungi all over.

- Bacteria actually tearing at the red cells.

- Lots of other small bits interpreted as proto-bacteria and whatever.

- Very pronounced fibrin forest in the back ground interpreted as caused by

blood acidity.

Since then, I have been on 19 day water fast, MMS, and homeopatics, minerals,

vitamins (not all concurrently, but some overlapping) between that exam and my

following one on 22.Dec.2009. I also had all my mercury fillings replaced with

white fillings during this period. This second DFM exam picture was as bleak as

the first one.

I took a holiday break with only topical magnesium and MMS topical maintenance

of toenail fungi and mouthwash.

I started a new regime on 9.Jan.2010. I used no supplements except for:

- Colloidal silver averaging 3 dcl a day, made by Sota Silver Pulsar.

- Magnesium oil applied topically 1/day every day over 1/3 of the body.

- Hydrogen peroxide starting on 5 drops/35%/2 per day, which I kept more or less

on 2x 10 drops a day through out, but which I jogged up over a few days to 25

drops 3x day by 2.Mar.2010.

- MMS topical maintenance of toenail fungi and mouthwash.

- Sota silver pulsar 70-80 min/ 2/day applied as recommended on the wrists.

- Sota magnetic pulsar 60 min 2/day two time split between locations, i.e. gall

bladder and lower abdomen 30 min each concurrently with the wrist pulsing.

I have abandoned the Silver pulsar on 26.Feb.2010 in favor of Godzilla. Godzilla

applied between the sole of my left leg and the same leg knee at 2.4V ~ 30 " ^2

pads, sole negative, no repolarization during the treatment.

Third dark field microscopy exam on 2.Mar.2010:

- Red blood cells all free, not two cells found stacked :-O

- No red blood cells torn :-O, but some misshapen :-(

- Only residual small clumps of fungi :-)

- No developed bacteria found :-O

- Still some small bits of whatever interpreted as proto-bacteria and whatever

:-(

- Fibrin forest barely noticeable interpreted as balanced PH :-O

My naturopath was very impressed and so was his colleague who looked through it

as well. I have ended up giving them a " lecture " on blood electrification. I was

further asked to be kind enough and send them whatever I may further get on the

theme :-)

A wart on my middle right hand finger, which I have been using as a visible

barometer of fungal infestation in my body has initially shrunk a bit on Silver

pulsar, but this had stopped around beginning of February. The wart has started

shrinking again two days after I've switched between Silver pulsar and Godzilla

and I have not run Godzilla anywhere near this wart, or put anything on it. The

wart is almost gone now 3.Mar.2010.

My gall bladder gives me less and less difficulties, which I assign to daily 1/2

hour Sota magnetic pulsar probably in combination with electrifying.

My abdominal cavity pains and discomfort appear to be diminishing, but I am not

quite certain. It has always been an on and off problem

I have jogged Godzilla voltage on 2.Mar.2010 knee to sole from 2.4V to 4.5V,

still with some 30 " ^2 pads with no polarity changes. It temporarily stiffens my

calf muscles a bit after treatment.

Somewhere along this route, I have got ridden of a lot of miscellaneous problems

and find it not only beneficial, but very interesting.

With kind regards, Slavek.

[Guest guest]

i'm curious. did your toenail fungus go away with the MMS? how much did

you put on and for how long?

thanks

monique

[Guest guest]

Hi ,

The MMS works like a charm. It took 2 weeks to begin to see healthy

toenail coming out. The slight problem is, that it has to be maintained

till all the bad nail is grown off, otherwise it gets reinfected in a

few weeks.

I used 6 drops MMS1 with 30 drops of citric acid, let react for ~ 3

minutes as per Jim Humble. Then add 72 drops of DMSO. Apply with an eye

dropper and spread with hands.

Now, this is a fair amount and I used it on the toenails both feet,

healthy as well as the bad ones. I have also spread it over my feet and

lower legs. I did have a " athlete's foot, if not worse. I applied it 2x

a day, but once a day is probably sufficient.

I had some dry blisters appear on my soles and sides of my feet, but

they healed within two weeks from the beginning of applications without

a trace and my feet are good ever since. Mind you, I apply the same dose

about once a week now for maintenance, because I found out the hard way

that the dead nails must first grow off completely, before one can

stop. Most people take over 1/2 year for the nails to fully grow off,

but the MMS does not hurt or anything, on intact skin at least.

It is a great stuff.

With kind regards, Slavek.

Sauve wrote:

>

>

>

> i'm curious. did your toenail fungus go away with the MMS? how much

> did

> you put on and for how long?

>

> thanks

> monique

>

>

[Guest guest]

Hello Salvek,

Wonderful report!!!

Where did you get access to a Darkfield microscope? Are there any in the U.S.?

I've read about them during the past few years. I'm sure that the 'medical

establishment' isn't using them.

MMS is truly a major 21st Century find!

I see the fasting and the rest of your tectics. I believe strongly in electronic

healing. But, as you said, how does one treat the whole body?

1)I think that several different types of electronic treatments do different

things. There are many cross-overs.

2) As with your use of MMS, there are chemical products that also do a job. CS

is certainly an important find. Both peroxide and ozone are very helpful. Many

cross-over points here too.

3) Then there is nutrition! Have you addressed this issue yet? I've read about

people reversing most problems and diseases with a juice fast.

4) Where Beck started was with his Protocol. The synergy of what you are doing

and what it accomplishes should be categorized.

My personal perspective is that the body is too complicated to do " everything "

with one type of treatment, or one diet, or even one protocol. Any number of

tactics are necessary.

Dick

>

> Hi ,

>

> The MMS works like a charm. It took 2 weeks to begin to see healthy

> toenail coming out. The slight problem is, that it has to be maintained

> till all the bad nail is grown off, otherwise it gets reinfected in a

> few weeks.

>

> I used 6 drops MMS1 with 30 drops of citric acid, let react for ~ 3

> minutes as per Jim Humble. Then add 72 drops of DMSO. Apply with an eye

> dropper and spread with hands.

>

> Now, this is a fair amount and I used it on the toenails both feet,

> healthy as well as the bad ones. I have also spread it over my feet and

> lower legs. I did have a " athlete's foot, if not worse. I applied it 2x

> a day, but once a day is probably sufficient.

>

> I had some dry blisters appear on my soles and sides of my feet, but

> they healed within two weeks from the beginning of applications without

> a trace and my feet are good ever since. Mind you, I apply the same dose

> about once a week now for maintenance, because I found out the hard way

> that the dead nails must first grow off completely, before one can

> stop. Most people take over 1/2 year for the nails to fully grow off,

> but the MMS does not hurt or anything, on intact skin at least.

>

> It is a great stuff.

>

> With kind regards, Slavek.

[Guest guest]

I use 1% clotrimazole plus dmso about 50-50 apply every few days with artist's

brush. it works. No idea if the dmso is actually required, but using it anyway.

bG

> >

> >

> >

> > i'm curious. did your toenail fungus go away with the MMS? how much

> > did

> > you put on and for how long?

> >

> > thanks

> > monique

> >

> >

>

[Guest guest]

Using Darkfield Microscopy To Enhance Contrast:

An Easy and Inexpensive Method

Charlotte K. Omoto*

*Department of Genetics and Cell Biology

Joan Folwell#

#Department of Zoology

Washington State University

P. O. Box 644234

Pullman, WA 99164-4234

Voice: (509) 335-5591

Fax: (509) 335-1907

omoto@...

--------------------------------------------------------------------------------

INTRODUCTION

Light microscopy is an important investigative tool for biology that is used

regularly in high schools and colleges. The structure of many biological

specimens are of low contrast that cannot be revealed by the brightfield

compound microscopes which are provided in many classrooms. Microscopes that

improve the contrast of these specimens through special optics are

prohibitively expensive for most teaching budgets. This article describes a

simple, inexpensive modification that changes a brightfield microscope into

a darkfield microscope allowing low contrast samples to be examined. This

article explains the basic theory of darkfield microscopy. Photographs

comparing brightfield and darkfield application are presented. This

technique allows expanded use of the brightfield microscope at all levels of

teaching with very little manipulation or expense.

Theory of darkfield microscopy

Microscopes are used to magnify objects. Through magnification, an image is

made to appear larger than the original object. The magnification of an

object can be calculated roughly by multiplying the magnification of the

objective lens times the magnification of the ocular lens. Objects are

magnified to be able to see small details. There is no limit to the

magnification that can be achieved; however, there is a magnification beyond

which detail does not become clearer. The result is called empty

magnification when objects are made bigger but their details do not become

clearer. Therefore, not only magnification but resolution is important to

the quality of the information in an image.

The resolving power of the microscope is defined as the ability to

distinguish two points apart from each other. The resolution of a microscope

is dependent on a number of factors in its construction. There is also an

inherent theoretical limit to resolution imposed by the wavelength of

visible light (400-600nm). The theoretical limit of resolution (the smallest

distance able to be seen between two points) is calculated as:

Resolution = 0.61 l/N.A.

where l represents the wavelength of light used and N.A.is the numerical

aperture. The student-grade microscopes generally have much lower resolution

than the theoretical limit because of lower quality lenses and illumination

systems.

Standard brightfield microscopy relies upon light from the lamp source being

gathered by the substage condenser and shaped into a cone whose apex is

focused at the plane of the specimen. Specimens are seen because of their

ability to change the speed and the path of the light passing through them.

This ability is dependent upon the refractive index and the opacity of the

specimen. To see a specimen in a brightfield microscope, the light rays

passing through it must be changed sufficiently to be able to interfere with

each other which produces contrast (differences in light intensities) and,

thereby, build an image. If the specimen has a refractive index too similar

to the surrounding medium between the microscope stage and the objective

lens, it will not be seen. To visualize biological materials well, the

materials must have this inherent contrast caused by the proper refractive

indices or be artificially stained. These limitations require instructors to

find naturally high contrast materials or to enhance contrast by staining

them which often requires killing them. Adequately visualizing transparent

living materials or thin unstained specimens is not possible with a

brightfield microscope.

Darkfield microscopy relies on a different illumination system. Rather than

illuminating the sample with a filled cone of light, the condenser is

designed to form a hollow cone of light. The light at the apex of the cone

is focused at the plane of the specimen; as this light moves past the

specimen plane it spreads again into a hollow cone. The objective lens sits

in the dark hollow of this cone; although the light travels around and past

the objective lens, no rays enter it (Fig. 1). The entire field appears dark

when there is no sample on the microscope stage; thus the name darkfield

microscopy. When a sample is on the stage, the light at the apex of the cone

strikes it. The image is made only by those rays scattered by the sample and

captured in the objective lens (note the rays scattered by the specimen in

Figure 1). The image appears bright against the dark background. This

situation can be compared to the glittery appearance of dust particles in a

dark room illuminated by strong shafts of light coming in through a side

window. The dust particles are very small, but are easily seen when they

scatter the light rays. This is the working principle of darkfield

microscopy and explains how the image of low contrast material is created:

an object will be seen against a dark background if it scatters light which

is captured with the proper device such as an objective lens.

The highest quality darkfield microscopes are equipped with specialized

costly condensers constructed only for darkfield application. This darkfield

effect can be achieved in a brightfield microscope, however, by the addition

of a simple " stop " . The stop is a piece of opaque material placed below the

substage condenser; it blocks out the center of the beam of light coming

from the base of the microscope and forms the hollow cone of light needed

for darkfield illumination.

Procedures

One example of a darkfield stop is shown in Figure 1; this is a " spider "

stop available for an Olympus CH series brightfield student-grade

microscope. The Olympus system has a color filter holder below the substage

condenser which can be removed; the color filter is replaced with the

darkfield stop and the holder is placed back into position. Other

manufacturers have slightly different designs. Some manufacturers include

this simple stop with the purchase of the microscope.

--------------------------------------------------------------------------------

LIST OF MICROSCOPE MANUFACTURERS

1.. Carl Zeiss USA

Microscopy Division

1-800-233-2343

(ask for name and number of local representative)

2.. Leica Microsystems Incorporated

111 Deer Lake Road

Deerfield, IL 60015

1-800-248-0123

(includes American Optical, Bausch and Lomb, Leitz, Reichert, and Wild

products)

3.. Meiji Techno America

2186 Bering Drive

San , CA 95131-2013

1-408-428-9654

4.. Nikon, Incorporated

Instrument Group

1300 Walt Whitman Road

Melville, NY 11747-3064

1-516-547-8500

5.. Olympus America Incorporated

Precision Instruments Division

4 Nevada Drive

Lake Success, NY 11402-1179

1-800-455-8236

or contact known area representatives of these companies.

--------------------------------------------------------------------------------

If a manufactured darkfield stop is not available for your microscopes,

there are some alternatives. If there is a filter holder below the

condenser, a dark field stop from another company may fit or be made to fit.

A coin or a circle of other opaque material can be mounted in the center of

a clear disk, e.g., glass, and inserted. A stop can also be fashioned by

punching out a circle of black construction paper; the circle is attached to

the bottom of the condenser with double-stick tape. This alternative can be

a bit tricky because the material needs to be placed in the center of the

condenser and the condenser needs to be cleaned when the tape is removed.

Technically, to properly block the beam, the stop should vary in diameter

from 8mm to 20mm depending on the magnification and numerical aperture of

the objective lens.

Darkfield microscopy reduces the amount of light entering the lens system of

a microscope in two ways. First, the stop blocks the center of the beam of

light that would otherwise fill the objective lens. Second, only the light

which is scattered by the specimen and enters the objective lens is seen.

Therefore, the best viewing result requires increasing the light intensity

as much as possible: by setting the light intensity adjustment at maximum,

by opening the field diaphragm, by opening the condenser aperture, and by

removing any color or other filters. The correct microscope slides also

should be used; they should be 1mm thick.

The illumination needs to be aligned and adjusted to achieve the best image.

Before making the darkfield modification, align the light beam in the center

of the field of view according to the manufacturer¹s instructions. To

facilitate focusing the substage condenser and the objective lens, use a

slide filled with samples that are easy to find; instructions for making a

cheek cell slide follow. Focus the sample slide at low magnification (10X)

in the brightfield mode. Insert the darkfield stop without changing the

focus. Make sure that the maximum amount of light is available. Rack up the

condenser to its highest position with the condenser focus knob. Look at the

sample and slowly lower the condenser until the sample is visible against a

dark background and in sharpest contrast. Finally, adjust the view of the

image with the fine focus knob.

Limitation of darkfield microscopy

The advantage of darkfield microscopy also becomes its disadvantage: not

only the specimen, but dust and other particles scatter the light and are

easily observed. For example, not only the cheek cells but the bacteria in

saliva are evident in Figure 2D. More care in sample preparation needs to be

exercised in darkfield application. Glass slides need to be thoroughly

cleaned of extraneous dust and dirt. It may be necessary to filter sample

media (agar, water, saline) to exclude confusing contaminants. Sample

materials need to be spread thinly; too much material on the slide creates

many overlapping layers and edges making it difficult to interpret

structures.

To create the image, this technique relies on scattered light from

specimens. Color is lacking or minimal; this can be disappointing to the

viewer. The actual size of specimens is also impacted; the width of objects

becomes exaggerated.

Examples of darkfield images.

Figure 2 compares the images of living Chlamydomonas, a biflagellate algae

in brightfield and in darkfield modes. Although the cells are seen with

brightfield, the flagella are not discernible (Fig. 2B). Some contrast can

be achieved by closing down the condenser aperture diaphragm which then

allows the flagella to be seen (Fig. 2A). However, closing down the

condenser aperture decreases the numerical aperture of objective lens

effectively reducing the resolution. Darkfield allows flagella and details

inside the to be seen distinctly (Fig. 2C). The use of darkfield microscopy

thus achieves both high contrast and high resolution.

Cheek cells make a quick study. Buccal cells are obtained by gently scraping

the inside of the mouth with a toothpick and thinly spreading the cells on a

slide; a cover slip is placed over the preparation which is not allowed to

dry. These cells have no inherent contrast and are difficult to see with a

brightfield. Dramatic contrast is achieved in a darkfield microscope; the

nucleus and other intracellular inclusions as well as bacteria in the

surrounding medium can be easily located and identified (Fig. 2D).

--------------------------------------------------------------------------------

[Guest guest]

Hi Dick,

I would say that most naturopathic clinics would have one. It is nothing

special. It is a high magnification optical microscope, I believe 6

000x, but the difference is, that the light is not directed orthogonally

through the sample as per orthodox method, but under an angle I believe

something like 30 degrees. This creates shading, which makes it much

easier to see the texture of the sample. My ND showed me the same

sample in both illuminations and the difference is comparable to the

photo of a person taken against the sun and with the sun. They should

have a camera on the microscope showing the image on the computer

screen. It is so obvious.

I would contact your American Association of Naturopathic Medicine,

whatever they call themselves, and they are likely to give you list of

clinics in your area, which have the equipment.

How does one treat the whole body is more a question of the equipment

size, then anything else. It is doable, but there is only one best way

to skin the cat, that is the simplest. I am still pondering and asking

questions. It will come to me one of these days.

From all the other things I did so far, the second best was the long

water fast. I went through my last one [4 days] a few days before this

DFM test, but I do not recommend it together with the peroxide and the

electrification. It really gets to you. You may call it Hexhimer, I

would just call it too many good things at once. It brought me to my

knees when it comes to being tired.

I have no soft spot for juicing. It sure helps, but I find it

impractical. Too much hassle and expense to get to real veggies and too

much hassle doing the juicing itself. It does not shut down your

digestive system and still keeps feeding the intestinal flora, whatever

it may be.

Straight long water fast [21 days] with enemas is a much more efficient

detox. Shutting down the stomach is a sure way to get rid of stomach

ulcers and whatever damage to the intestinal walls. It allows all of

this to heal.

With kind regards, Slavek.

luthierret wrote:

>

>

>

> Hello Salvek,

>

> Wonderful report!!!

> Where did you get access to a Darkfield microscope? Are there any in

> the U.S.? I've read about them during the past few years. I'm sure

> that the 'medical establishment' isn't using them.

>

> MMS is truly a major 21st Century find!

>

> I see the fasting and the rest of your tectics. I believe strongly in

> electronic healing. But, as you said, how does one treat the whole

> body?

>

> 1)I think that several different types of electronic treatments do

> different things. There are many cross-overs.

> 2) As with your use of MMS, there are chemical products that also do a

> job. CS is certainly an important find. Both peroxide and ozone are

> very helpful. Many cross-over points here too.

> 3) Then there is nutrition! Have you addressed this issue yet? I've

> read about people reversing most problems and diseases with a juice

> fast.

> 4) Where Beck started was with his Protocol. The synergy of what you

> are doing and what it accomplishes should be categorized.

>

> My personal perspective is that the body is too complicated to do

> " everything " with one type of treatment, or one diet, or even one

> protocol. Any number of tactics are necessary.

>

> Dick

>

>

> >

> > Hi ,

> >

> > The MMS works like a charm. It took 2 weeks to begin to see healthy

> > toenail coming out. The slight problem is, that it has to be

> maintained

> > till all the bad nail is grown off, otherwise it gets reinfected in

> a

> > few weeks.

> >

> > I used 6 drops MMS1 with 30 drops of citric acid, let react for ~ 3

> > minutes as per Jim Humble. Then add 72 drops of DMSO. Apply with an

> eye

> > dropper and spread with hands.

> >

> > Now, this is a fair amount and I used it on the toenails both feet,

> > healthy as well as the bad ones. I have also spread it over my feet

> and

> > lower legs. I did have a " athlete's foot, if not worse. I applied it

> 2x

> > a day, but once a day is probably sufficient.

> >

> > I had some dry blisters appear on my soles and sides of my feet, but

> > they healed within two weeks from the beginning of applications

> without

> > a trace and my feet are good ever since. Mind you, I apply the same

> dose

> > about once a week now for maintenance, because I found out the hard

> way

> > that the dead nails must first grow off completely, before one can

> > stop. Most people take over 1/2 year for the nails to fully grow

> off,

> > but the MMS does not hurt or anything, on intact skin at least.

> >

> > It is a great stuff.

> >

> > With kind regards, Slavek.

>

>

[Guest guest]

Just a word about the DarkField microscope;

Off the subject of Beck (again), another important researcher, Royal Rife,

started his process by developing a microscope that could see viruses in motion.

The concept differs from " modern " technology, the electron microscope in that

regard because the electron microscope fries or cooks everything that it is

observing.

, who has a German web site on Rife, was one of the people involved

in developing this instrument.It should allow researchers to fine tune all sorts

of healing, including frequency healing.

I was just surprised to see it on the market. Another researcher, Stan Truman,

who also has a web site on Rife, has developed another microscope along those

lines. I've read about a third effort using photons. It was only one blurb. The

author stated that he had concluded that photons were what Rife had originally

used with his microscope.

>

> Hi Dick,

>

> I would say that most naturopathic clinics would have one. It is nothing

> special. It is a high magnification optical microscope, I believe 6

> 000x, but the difference is, that the light is not directed orthogonally

> through the sample as per orthodox method, but under an angle I believe

> something like 30 degrees. This creates shading, which makes it much

> easier to see the texture of the sample. My ND showed me the same

> sample in both illuminations and the difference is comparable to the

> photo of a person taken against the sun and with the sun. They should

> have a camera on the microscope showing the image on the computer

> screen. It is so obvious.

>

> I would contact your American Association of Naturopathic Medicine,

> whatever they call themselves, and they are likely to give you list of

> clinics in your area, which have the equipment.

>

> How does one treat the whole body is more a question of the equipment

> size, then anything else. It is doable, but there is only one best way

> to skin the cat, that is the simplest. I am still pondering and asking

> questions. It will come to me one of these days.

>

> From all the other things I did so far, the second best was the long

> water fast. I went through my last one [4 days] a few days before this

> DFM test, but I do not recommend it together with the peroxide and the

> electrification. It really gets to you. You may call it Hexhimer, I

> would just call it too many good things at once. It brought me to my

> knees when it comes to being tired.

>

> I have no soft spot for juicing. It sure helps, but I find it

> impractical. Too much hassle and expense to get to real veggies and too

> much hassle doing the juicing itself. It does not shut down your

> digestive system and still keeps feeding the intestinal flora, whatever

> it may be.

>

> Straight long water fast [21 days] with enemas is a much more efficient

> detox. Shutting down the stomach is a sure way to get rid of stomach

> ulcers and whatever damage to the intestinal walls. It allows all of

> this to heal.

>

> With kind regards, Slavek.