A Rapid Real-time PCR Assay for Determination of HCV Genotypes 1, 2 and 3a

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A Rapid Real-time PCR Assay for Determination of HCV Genotypes 1, 2

and 3a

The genotypes of hepatitis C virus (HCV) in serum of patients have

been described as independent predictors of success of antiviral

therapy. Therefore, different antiviral regimens have been proposed

depending on the infecting HCV genotype.

HCV strain is usually determined by polymerase chain reaction (PCR)

amplification of genome followed by sequencing or by line-probe

assays.

In the current study, Norwegian researchers report a new one step

real-time PCR assay for genotyping of HCV strains that are prevalent

in patients in Norway.

HCV types 1, 2 and 3a were genotyped in 37 patient serum samples with

100% correlation to genotyping by nucleotide sequence analysis and

line-probe assays.

Genotyping was also confirmed with an HCV genotype panel from the

National Institute for Biological Standards and Control.

This assay does not require manipulation of amplified PCR products,

and it involves very little hands on and analysis time.

The study authors conclude, " This assay can be used for rapid

genotyping of HCV-RNA in infected patients to aid physicians decide

suitability of patients for treatment and subsequent length of

treatment. "

For more information, read the full text of the article

(See " Reference " below). You may also email the lead investigator of

the study, Amir Moghaddam, at amoghaddam@...

11/07/06