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Perforin

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One of the talks at the Seattle meeting that I found particularly

interesting was by Dr. Maher, who is in Dr. Klimas's

immunology group in Miami. found that the natural killer cells

in PWCs are low in perforin. Perforin is the protein that natural

killer cells and cytotoxic (CD-8) T cells use to " perforate " the

membranes of infected cells in order to drive them into apoptosis

(controlled cell death). This is how viral and intracellular

infections are normally countered.

I think this might explain why PWCs have low NK cell function and poor

Th1 immune response, and why they are plagued with viral and

intracellular bacterial infections. is going to follow up on

this study and try to figure out why the perforin is low.

It is interesting to note that the perforin molecule has a

high-cysteine region. I have urged to investigate whether the

low perforin could be caused by low cysteine in the blood serum, which

is where the NK and T cells get their nutrients. If this turns out to

be the case, then there would appear to be a link between perforin

depletion and glutathione depletion, since the limiting substrate for

synthesis of glutathione is usually cysteine also. It would also

seem, then, that nondenatured whey protein might be the way to build

up the perforin and restore the NK and killer T cell activities.

Rich

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