A Second Look at RNA Interference

[Guest guest]

Review

Nature Reviews Genetics 7, 373-384 (May 2006) | doi:10.1038/nrg1836

High-throughput RNAi screening in cultured cells: a user's guide

Christophe J. Echeverri1 and Norbert Perrimon2 About the authors

Abstract

RNA interference has re-energized the field of functional genomics by

enabling genome-scale loss-of-function screens in cultured cells.

Looking back on the lessons that have been learned from the first

wave of technology developments and applications in this exciting

field, we provide both a user's guide for newcomers to the field and

a detailed examination of some more complex issues, particularly

concerning optimization and quality control, for more advanced users.

From a discussion of cell lines, screening paradigms, reagent types

and read-out methodologies, we explore in particular the complexities

of designing optimal controls and normalization strategies for these

challenging but extremely powerful studies.

Read the rest at:

http://www.nature.com/nrg/journal/v7/n5/full/nrg1836.html